Reviewed, UniProtKB/Swiss-Prot B4RW28 (KATG_ALTMD)

Names and origin

Protein names

Recommended name:
    Catalase-peroxidase
      Short name=CP
    EC=1.11.1.6
    EC=1.11.1.7
Alternative name(s):
    Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	MADE_01465

Organism

Alteromonas macleodii (strain DSM 17117 / Deep ecotype) [Complete proteome] [HAMAP]

Taxonomic identifier

314275 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Alteromonadaceae › Alteromonas

Protein attributes

Sequence length	781 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	2 H2O2 = O2 + 2 H2O. HAMAP MF_01961 Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity. HAMAP MF_01961
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Domain	Signal
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: HAMAP heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 41	41	Potential
	Chain	42 – 781	740	Catalase-peroxidase HAMAP MF_01961		PRO_0000354720
Sites
	Active site	138	1	Proton acceptor By similarity
	Metal binding	300	1	Iron (heme axial ligand) By similarity
	Site	134	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	137 ↔ 259		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-285) By similarity
	Cross-link	259 ↔ 285		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-137) By similarity

Sequences

	Sequence		Length	Mass (Da)
	B4RW28-1 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 57D74AE0AD4CC23B	FASTA	781	86,346
	10 20 30 40 50 60 MPQFDNENKL RREKTMNMNR FRKTTLSALV ALSLGATGAI ANDQISKPAG AKGTGMTKAF 70 80 90 100 110 120 QPKSNQFWWP DQLDLSPLRD HDNRSNPLGE DFDYAKAFSK LDLDQVKSDI NELLTTSQDW 130 140 150 160 170 180 WPADFGNYGP FFIRLSWHSA GTYRTLDGRG GGDGGQMRFD PLNSWPDNGN LDKARRLLWP 190 200 210 220 230 240 IKQKYGESLS WGDLMILAGT VGMENMGFDT YGFAGGRTDD WEPDMVYWGP EVEMLASDRE 250 260 270 280 290 300 EREGKLQRPL GATHMGLIYV NPEGPKGVPD PMGSAKNIRT AFARMAMNDE ETVALIAGGH 310 320 330 340 350 360 TFGKMHGAHK PADCLEAEPG GAGLEEQGLG WKNNCGKGNA EDTVTSGLEG AWTQLPTKWT 370 380 390 400 410 420 SLYLQNLLGF EWKQTRSPAG AIQWVPTDES LHTSVPDAHV EGKKNPPVMT TADLALKYDP 430 440 450 460 470 480 EYRKIAERFL ADPKEYQTAF AKAWFKLTHR DMGPKERYLG NDIPRENFIW QDPVPKADYK 490 500 510 520 530 540 TISDKDVKKL KADILDSGLT VQQLVKTAWA GASSFRASDL RGGANGARIA LEPQMSWEAN 550 560 570 580 590 600 EPETVKAVVA KLKELQEDYN SRMFSKKKVS LADLIVIGGA AAIEKAAADA GVEVSVPVVP 610 620 630 640 650 660 GRTDATQEQT DVNSFSLLEP TADAFRNYYN AEASYRSPTD MLVDKADQLN LTVPEMTVLL 670 680 690 700 710 720 GGLRSLGANT GGTNHGVFTD NVGTLNNDFF VTLLDMGVKW RKTDDASVYE GVNRSTGEVM 730 740 750 760 770 780 YTGTPVDLVF GSNSELRAVA EVYAYDNAKT RFVEDFVDAW TKVMTLDRFD LRHDLNAKLA K

References

[1]

"Comparative genomics of two ecotypes of the marine planktonic copiotroph Alteromonas macleodii suggests alternative lifestyles associated with different kinds of particulate organic matter." Ivars-Martinez E., Martin-Cuadrado A.-B., D'Auria G., Mira A., Ferriera S., Johnson J., Friedman R., Rodriguez-Valera F. ISME J. 2:1194-1212(2008) [PubMed: 18670397] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001103 Genomic DNA. Translation: ACG65776.1.
RefSeq	YP_002125770.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6775579.
GenomeReviews	Gene locus MADE_01465 in contig CP001103_GR.
KEGG	amc:MADE_01465.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG285610.
OMA	KNKCGKG.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
TIGRFAMs	TIGR00198. cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_ALTMD

Accession

Primary (citable) accession number: B4RW28

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	September 23, 2008
Last modified:	February 9, 2010
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)