B4RW28 (KATG_ALTMD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	MADE_1016605

Organism

Alteromonas macleodii (strain DSM 17117 / Deep ecotype) [Complete proteome] [HAMAP]

Taxonomic identifier

314275 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Alteromonadaceae › Alteromonas

Protein attributes

Sequence length	766 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Domain	Signal
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 766	740	Catalase-peroxidase HAMAP MF_01961		PRO_0000354720
Sites
	Active site	123	1	Proton acceptor By similarity
	Metal binding	285	1	Iron (heme axial ligand) By similarity
	Site	119	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	122 ↔ 244		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-270) By similarity
	Cross-link	244 ↔ 270		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-122) By similarity

Sequences

	Sequence		Length	Mass (Da)
	B4RW28 [UniParc]. Last modified October 19, 2011. Version 2. Checksum: DB68CFA186110ADF	FASTA	766	84,458
	10 20 30 40 50 60 MNMNRFRKTT LSALVALSLG ATGAIANDQI SKPAGAKGTG MTKAFQPKSN QFWWPDQLDL 70 80 90 100 110 120 SPLRDHDNRS NPLGEDFDYA KAFSKLDLDQ VKSDINELLT TSQDWWPADF GNYGPFFIRL 130 140 150 160 170 180 SWHSAGTYRT LDGRGGGDGG QMRFDPLNSW PDNGNLDKAR RLLWPIKQKY GESLSWGDLM 190 200 210 220 230 240 ILAGTVGMEN MGFDTYGFAG GRTDDWEPDM VYWGPEVEML ASDREEREGK LQRPLGATHM 250 260 270 280 290 300 GLIYVNPEGP KGVPDPMGSA KNIRTAFARM AMNDEETVAL IAGGHTFGKM HGAHKPADCL 310 320 330 340 350 360 EAEPGGAGLE EQGLGWKNNC GKGNAEDTVT SGLEGAWTQL PTKWTSLYLQ NLLGFEWKQT 370 380 390 400 410 420 RSPAGAIQWV PTDESLHTSV PDAHVEGKKN PPVMTTADLA LKYDPEYRKI AERFLADPKE 430 440 450 460 470 480 YQTAFAKAWF KLTHRDMGPK ERYLGNDIPR ENFIWQDPVP KADYKTISDK DVKKLKADIL 490 500 510 520 530 540 DSGLTVQQLV KTAWAGASSF RASDLRGGAN GARIALEPQM SWEANEPETV KAVVAKLKEL 550 560 570 580 590 600 QEDYNSRMFS KKKVSLADLI VIGGAAAIEK AAADAGVEVS VPVVPGRTDA TQEQTDVNSF 610 620 630 640 650 660 SLLEPTADAF RNYYNAEASY RSPTDMLVDK ADQLNLTVPE MTVLLGGLRS LGANTGGTNH 670 680 690 700 710 720 GVFTDNVGTL NNDFFVTLLD MGVKWRKTDD ASVYEGVNRS TGEVMYTGTP VDLVFGSNSE 730 740 750 760 LRAVAEVYAY DNAKTRFVED FVDAWTKVMT LDRFDLRHDL NAKLAK

References

[1]

"Comparative genomics of two ecotypes of the marine planktonic copiotroph Alteromonas macleodii suggests alternative lifestyles associated with different kinds of particulate organic matter." Ivars-Martinez E., Martin-Cuadrado A.-B., D'Auria G., Mira A., Ferriera S., Johnson J., Friedman R., Rodriguez-Valera F. ISME J. 2:1194-1212(2008) [PubMed: 18670397] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 17117 / Deep ecotype.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001103 Genomic DNA. Translation: AEA99445.1.
RefSeq	YP_004428443.1. NC_011138.2.
3D structure databases
ProteinModelPortal	B4RW28.
ModBase	Search...
Protein-protein interaction databases
STRING	B4RW28.
Protein family/group databases
PeroxiBase	7308. AmacCP01.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	10558662.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG285610.
OMA	KNKCGKG.
ProtClustDB	PRK15061.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_ALTMD

Accession

Primary (citable) accession number: B4RW28
Secondary accession number(s): F2G4D5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	October 19, 2011
Last modified:	January 25, 2012
This is version 28 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program