Reviewed, UniProtKB/Swiss-Prot B4RVY8 (DXS_ALTMD)

Names and origin

Protein names

Recommended name:
    1-deoxy-D-xylulose-5-phosphate synthase
    EC=2.2.1.7
Alternative name(s):
    1-deoxyxylulose-5-phosphate synthase
      Short name=DXP synthase
      Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	MADE_01425

Organism

Alteromonas macleodii (strain DSM 17117 / Deep ecotype) [Complete proteome] [HAMAP]

Taxonomic identifier

314275 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Alteromonadaceae › Alteromonas

Protein attributes

Sequence length	625 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity.
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP thiamin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 625	625	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_1000115718

Sequences

	Sequence		Length	Mass (Da)
	B4RVY8-1 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: A1D039E0381AD915	FASTA	625	67,725
	10 20 30 40 50 60 MSLDLQHYPT LALAQTPDKL RQLPQDKLRE LADELREYLL NSVSQTSGHF ASGLGTVELT 70 80 90 100 110 120 VALHYVYNTP FDRLLWDVGH QAYPHKILTG RAERMSTIRQ KNGLHPFPWP PESEYDTFAV 130 140 150 160 170 180 GHSSTSISAA LGMAVAAEKE GKDRKVVSVI GDGAMTAGMA FEALNHAGDI KKDMVVVLND 190 200 210 220 230 240 NEMSISENVG ALNSHLARLL TGNFFNSIRD GGKKLLSNVP PIKEFASRAE EHLKGMVVPG 250 260 270 280 290 300 TIFEELGFNY IGPIDGHDVN AVVDTLRNMR NFDGPQLLHV VTKKGKGYAV AEEDPIKFHA 310 320 330 340 350 360 VPKFNPADNA LPKSKPSAPT YSAIFGQWLC DMAAQDPKLM AVTPAMREGS GMVEFSQRFP 370 380 390 400 410 420 EQYFDVAIAE QHAVTFGAGL AKDGMNAVVA IYSSFLQRAY DQLIHDVAIQ DLPVLFAIDR 430 440 450 460 470 480 AGIVGADGPT HQGAFDIAFL RCIPNMVVMA PSDENECRQM LYTGHKLQKP AAVRYPRGAG 490 500 510 520 530 540 MGVTPDEAMT ALEIGKSRTC RETAKDKSES VAILNFGCLL PYALEAAVAI DATVIDMRFI 550 560 570 580 590 600 KPLDGDAVLK AANEHSALIT LEDGCIMGGA GSAVLEHLQQ NGVLKAVKML GLPDSFILQG 610 620 TQQEMYKEHG LDAEGIIAAA KSLIG

References

[1]

"Comparative genomics of two ecotypes of the marine planktonic copiotroph Alteromonas macleodii suggests alternative lifestyles associated with different kinds of particulate organic matter." Ivars-Martinez E., Martin-Cuadrado A.-B., D'Auria G., Mira A., Ferriera S., Johnson J., Friedman R., Rodriguez-Valera F. ISME J. 2:1194-1212(2008) [PubMed: 18670397] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001103 Genomic DNA. Translation: ACG65736.1.
RefSeq	YP_002125730.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6779221.
GenomeReviews	Gene locus MADE_01425 in contig CP001103_GR.
KEGG	amc:MADE_01425.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	QRFPDRY.
Family and domain databases
HAMAP	MF_00315. [Tree]
InterPro	IPR005477. Dxylulose-5-P_synthase. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
Pfam	PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00204. dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_ALTMD

Accession

Primary (citable) accession number: B4RVY8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 23, 2008
Last modified:	November 3, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)