Reviewed, UniProtKB/Swiss-Prot B4RVP1 (LSPA_ALTMD)

Names and origin

Protein names

Recommended name:
    Lipoprotein signal peptidase
    EC=3.4.23.36
Alternative name(s):
    Prolipoprotein signal peptidase
    Signal peptidase II
      Short name=SPase II

Gene names

Name:	lspA
Ordered Locus Names:	MADE_03029

Organism

Alteromonas macleodii (strain DSM 17117 / Deep ecotype) [Complete proteome] [HAMAP]

Taxonomic identifier

314275 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Alteromonadaceae › Alteromonas

Protein attributes

Sequence length	182 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This protein specifically catalyzes the removal of signal peptides from prolipoproteins By similarity.
Catalytic activity	Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains. HAMAP MF_00161
Pathway	Protein modification; lipoprotein biosynthesis (signal peptide cleavage). HAMAP MF_00161
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the peptidase A8 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Molecular function	Aspartyl protease Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 182	182	Lipoprotein signal peptidase HAMAP MF_00161		PRO_1000097227
Regions
	Transmembrane	12 – 32	21	Potential
	Transmembrane	40 – 60	21	Potential
	Transmembrane	70 – 90	21	Potential
	Transmembrane	97 – 117	21	Potential
	Transmembrane	136 – 156	21	Potential
Sites
	Active site	114	1	By similarity
	Active site	141	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B4RVP1-1 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 446721D2161343EC	FASTA	182	20,693
	10 20 30 40 50 60 MLKLFRETGL RFLWISALAF ILDQWSKYTV IDTMSLYQSI QVLPFFNFTY VHNYGAAFSF 70 80 90 100 110 120 LENAGGWQRW FFTAIAVVVS VVILWWLKQS PRSQKMLPVA FAFILGGALG NVYDRLVHGY 130 140 150 160 170 180 VIDFLDFYVN NYHWPAFNIA DSAIFIGAAL LIIDMFKNGD KKSEENGAES KAGSANSSET IK

References

[1]

"Comparative genomics of two ecotypes of the marine planktonic copiotroph Alteromonas macleodii suggests alternative lifestyles associated with different kinds of particulate organic matter." Ivars-Martinez E., Martin-Cuadrado A.-B., D'Auria G., Mira A., Ferriera S., Johnson J., Friedman R., Rodriguez-Valera F. ISME J. 2:1194-1212(2008) [PubMed: 18670397] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001103 Genomic DNA. Translation: ACG67323.1.
RefSeq	YP_002127317.1.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	A08.001.
Genome annotation databases
GeneID	6777597.
GenomeReviews	Gene locus MADE_03029 in contig CP001103_GR.
KEGG	amc:MADE_03029.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	ADSSGWQ.
Family and domain databases
HAMAP	MF_00161. [Tree]
InterPro	IPR001872. Peptidase_A8. [Graphical view]
Pfam	PF01252. Peptidase_A8. 1 hit. [Graphical view]
PRINTS	PR00781. LIPOSIGPTASE.
TIGRFAMs	TIGR00077. lspA. 1 hit.
PROSITE	PS00855. SPASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LSPA_ALTMD

Accession

Primary (citable) accession number: B4RVP1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 23, 2008
Last modified:	November 3, 2009
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)