B4RV80 (GUAA_ALTMD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GMP synthase [glutamine-hydrolyzing]
EC=6.3.5.2

Alternative name(s):
GMP synthetase
Glutamine amidotransferase

Gene names

Name:	guaA
Ordered Locus Names:	MADE_1005260

Organism

Alteromonas macleodii (strain DSM 17117 / Deep ecotype) [Complete proteome] [HAMAP]

Taxonomic identifier

314275 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Alteromonadaceae › Alteromonas

Protein attributes

Sequence length	525 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_00344
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_00344
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_00344
Subunit structure	Homodimer By similarity. HAMAP MF_00344
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain. Contains 1 GMPS ATP-PPase (ATP pyrophosphatase) domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 525	525	GMP synthase [glutamine-hydrolyzing] HAMAP MF_00344		PRO_1000120208
Regions
	Domain	9 – 207	199	Glutamine amidotransferase type-1
	Domain	208 – 400	193	GMPS ATP-PPase
	Nucleotide binding	235 – 241	7	ATP By similarity
Sites
	Active site	86	1	Nucleophile By similarity
	Active site	181	1	By similarity
	Active site	183	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B4RV80 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: BECFF25F6820C715	FASTA	525	58,584
	10 20 30 40 50 60 MTTNIHDQRI LILDFGSQYT QLIARRVREI GVYCELWAWD VTEEQIREFN PQGIILSGGP 70 80 90 100 110 120 ESVTEEGSPR APDYVFEAGV PVLGVCYGMQ TMAHQLGGGV LGSDKREFGY AQVEVIKPSP 130 140 150 160 170 180 LLDKIEDAIG DNGAALLDVW MSHGDKVAAI PEGFETIAQT ASCPHAAMYN EEKQFYGVQF 190 200 210 220 230 240 HPEVTHTRQG MRLLTQFVMD ICKCEKLWTA GAIIEDAIER IKEKVGDERV ILGLSGGVDS 250 260 270 280 290 300 SVTAMLLHRA IGKNLTCVFV DNGLLRLNEA DQVMEMFGDH FGLNIIRVDA EGRFLDRLKG 310 320 330 340 350 360 IEDPELKRKA IGNEFVRVFD EEASKLENAK WLAQGTIYPD VIESAGSATG KAHVIKSHHN 370 380 390 400 410 420 VGGLPEDMEL GLVEPLRELF KDEVRKIGLE LGLPYDMLYR HPFPGPGLGV RVLGEIKKEY 430 440 450 460 470 480 CDLLRRADAI FIEELYAADL YQKVSQAFTV FLPVRSVGVM GDARKYDWVV SLRAVETIDF 490 500 510 520 MTAHWAHLPY DFLGKVSNRI INEIDGISRV VYDISGKPPA TIEWE

References

[1]

"Comparative genomics of two ecotypes of the marine planktonic copiotroph Alteromonas macleodii suggests alternative lifestyles associated with different kinds of particulate organic matter." Ivars-Martinez E., Martin-Cuadrado A.-B., D'Auria G., Mira A., Ferriera S., Johnson J., Friedman R., Rodriguez-Valera F. ISME J. 2:1194-1212(2008) [PubMed: 18670397] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 17117 / Deep ecotype.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001103 Genomic DNA. Translation: AEA97198.1.
RefSeq	YP_004426196.1. NC_011138.2.
3D structure databases
ProteinModelPortal	B4RV80.
SMR	B4RV80. Positions 4-525.
ModBase	Search...
Protein-protein interaction databases
STRING	B4RV80.
Protein family/group databases
MEROPS	C26.A17.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	10556393.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG391775.
OMA	DGRTYEY.
ProtClustDB	PRK00074.
Family and domain databases
HAMAP	MF_00344. GMP_synthase. [Tree]
InterPro	IPR017926. GATASE_1. IPR001674. GMP_synth_C. IPR004739. GMP_synth_N. IPR022955. GMP_synthase. IPR022310. NAD/GMP_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF00117. GATase. 1 hit. PF00958. GMP_synt_C. 1 hit. PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00884. GuaA_Cterm. 1 hit. TIGR00888. GuaA_Nterm. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. PS51553. GMPS_ATP_PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAA_ALTMD

Accession

Primary (citable) accession number: B4RV80
Secondary accession number(s): F2G264

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 23, 2008
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program