Reviewed, UniProtKB/Swiss-Prot B4RUH2 (T23O_ALTMD)

Names and origin

Protein names

Recommended name:
    Tryptophan 2,3-dioxygenase
      Short name=TDO
    EC=1.13.11.11
Alternative name(s):
    Tryptophan pyrrolase
      Short name=Tryptophanase
    Tryptophan oxygenase
      Short name=TRPO
      Short name=TO
    Tryptamin 2,3-dioxygenase

Gene names

Name:	kynA
Ordered Locus Names:	MADE_02822

Organism

Alteromonas macleodii (strain DSM 17117 / Deep ecotype) [Complete proteome] [HAMAP]

Taxonomic identifier

314275 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Alteromonadaceae › Alteromonas

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring By similarity.
Catalytic activity	L-tryptophan + O2 = N-formyl-L-kynurenine.
Cofactor	Binds 2 heme groups per tetramer By similarity.
Pathway	Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the tryptophan 2,3-dioxygenase family.

Ontologies

Keywords
Biological process	Tryptophan catabolism
Ligand	Heme Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW protein homotetramerization Inferred from sequence or structural similarity. Source: UniProtKB tryptophan catabolic process to kynurenine Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	heme binding Inferred from sequence or structural similarity. Source: UniProtKB tryptophan 2,3-dioxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 362	362	Tryptophan 2,3-dioxygenase		PRO_0000360079
Regions
	Region	10 – 14	5	Substrate binding By similarity
	Region	40 – 44	5	Substrate binding By similarity
Sites
	Metal binding	297	1	Iron (heme axial ligand) By similarity
	Binding site	111	1	Substrate By similarity
	Binding site	118	1	Heme By similarity
	Binding site	311	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B4RUH2-1 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 955EDB34A2D407A2	FASTA	362	42,791
	10 20 30 40 50 60 MKKNIEPCYY GDYLQLDKIL GAQDLQSEKY GDGAHEEMLF IIVHQVYELW FKQVLHELNA 70 80 90 100 110 120 VIDTFNQEAV KDQQLTQVVH RLQRIIQIQK LMNDQIAIME TMTPQQFLSF RDYLVPASGF 130 140 150 160 170 180 QSIQFKRLEI SLGLKREFRI DFDKQSFYNR LTDKDRALLE NLENKPSLFE LVDKWLSRMP 190 200 210 220 230 240 LLKTEDFDFW QYYKDAADEM LKDDHHTVST SDMLSDTEKR QEIKDLQATM ENFDALFNET 250 260 270 280 290 300 QFEKLRGEGK FRLSHNALLS ALFIKQYSEE PIFNLPFQLI TALTEIDEQL TIWRYRHAMM 310 320 330 340 350 360 VQRMLGTKIG TGGSSGHHYL KKTTESNRIY LDFFNMATFL LPKSALPDLP ESVRRRLGFY LQ

References

[1]

"Comparative genomics of two ecotypes of the marine planktonic copiotroph Alteromonas macleodii suggests alternative lifestyles associated with different kinds of particulate organic matter." Ivars-Martinez E., Martin-Cuadrado A.-B., D'Auria G., Mira A., Ferriera S., Johnson J., Friedman R., Rodriguez-Valera F. ISME J. 2:1194-1212(2008) [PubMed: 18670397] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001103 Genomic DNA. Translation: ACG67118.1.
RefSeq	YP_002127112.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6776077.
GenomeReviews	Gene locus MADE_02822 in contig CP001103_GR.
KEGG	amc:MADE_02822.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG647485.
OMA	QANIEEG.
Family and domain databases
InterPro	IPR004981. Trp_2_3_dOase. [Graphical view]
PANTHER	PTHR10138. Trp_2_3_dOase. 1 hit.
Pfam	PF03301. Trp_dioxygenase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T23O_ALTMD

Accession

Primary (citable) accession number: B4RUH2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	September 23, 2008
Last modified:	December 15, 2009
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)