B4RUH2 (T23O_ALTMD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan 2,3-dioxygenase
Short name=TDO
EC=1.13.11.11

Alternative name(s):
Tryptamin 2,3-dioxygenase
Tryptophan oxygenase
Short name=TO
Short name=TRPO
Tryptophan pyrrolase
Tryptophanase

Gene names

Name:	kynA
Ordered Locus Names:	MADE_1006210

Organism

Alteromonas macleodii (strain DSM 17117 / Deep ecotype) [Complete proteome] [HAMAP]

Taxonomic identifier

314275 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Alteromonadaceae › Alteromonas

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring By similarity.
Catalytic activity	L-tryptophan + O2 = N-formyl-L-kynurenine.
Cofactor	Binds 2 heme groups per tetramer By similarity.
Pathway	Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the tryptophan 2,3-dioxygenase family.

Ontologies

Keywords
Biological process	Tryptophan catabolism
Ligand	Heme Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein homotetramerization Inferred from sequence or structural similarity. Source: UniProtKB tryptophan catabolic process to kynurenine Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	heme binding Inferred from sequence or structural similarity. Source: UniProtKB tryptophan 2,3-dioxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 362	362	Tryptophan 2,3-dioxygenase		PRO_0000360079
Regions
	Region	10 – 14	5	Substrate binding By similarity
	Region	40 – 44	5	Substrate binding By similarity
Sites
	Metal binding	297	1	Iron (heme axial ligand) By similarity
	Binding site	111	1	Substrate By similarity
	Binding site	118	1	Heme By similarity
	Binding site	311	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B4RUH2 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 955EDB34A2D407A2	FASTA	362	42,791
	10 20 30 40 50 60 MKKNIEPCYY GDYLQLDKIL GAQDLQSEKY GDGAHEEMLF IIVHQVYELW FKQVLHELNA 70 80 90 100 110 120 VIDTFNQEAV KDQQLTQVVH RLQRIIQIQK LMNDQIAIME TMTPQQFLSF RDYLVPASGF 130 140 150 160 170 180 QSIQFKRLEI SLGLKREFRI DFDKQSFYNR LTDKDRALLE NLENKPSLFE LVDKWLSRMP 190 200 210 220 230 240 LLKTEDFDFW QYYKDAADEM LKDDHHTVST SDMLSDTEKR QEIKDLQATM ENFDALFNET 250 260 270 280 290 300 QFEKLRGEGK FRLSHNALLS ALFIKQYSEE PIFNLPFQLI TALTEIDEQL TIWRYRHAMM 310 320 330 340 350 360 VQRMLGTKIG TGGSSGHHYL KKTTESNRIY LDFFNMATFL LPKSALPDLP ESVRRRLGFY LQ

References

[1]

"Comparative genomics of two ecotypes of the marine planktonic copiotroph Alteromonas macleodii suggests alternative lifestyles associated with different kinds of particulate organic matter." Ivars-Martinez E., Martin-Cuadrado A.-B., D'Auria G., Mira A., Ferriera S., Johnson J., Friedman R., Rodriguez-Valera F. ISME J. 2:1194-1212(2008) [PubMed: 18670397] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 17117 / Deep ecotype.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001103 Genomic DNA. Translation: AEA97384.1.
RefSeq	YP_004426382.1. NC_011138.2.
3D structure databases
ProteinModelPortal	B4RUH2.
ModBase	Search...
Protein-protein interaction databases
STRING	B4RUH2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	10556583.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG647485.
OMA	VPASGFQ.
ProtClustDB	CLSK2307017.
Family and domain databases
InterPro	IPR004981. Trp_2_3_dOase. [Graphical view]
PANTHER	PTHR10138. Trp_2_3_dOase. 1 hit.
Pfam	PF03301. Trp_dioxygenase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T23O_ALTMD

Accession

Primary (citable) accession number: B4RUH2
Secondary accession number(s): F2G4T0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	September 23, 2008
Last modified:	December 14, 2011
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program