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Protein

Imidazole glycerol phosphate synthase subunit HisF

Gene

hisF

Organism
Alteromonas mediterranea (strain DSM 17117 / CIP 110805 / LMG 28347 / Deep ecotype)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the HisH subunit.UniRule annotation

Catalytic activityi

5-[(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino]-1-(5-phosphoribosyl)imidazole-4-carboxamide + L-glutamine = imidazole-glycerol phosphate + 5-aminoimidazol-4-carboxamide ribonucleotide + L-glutamate + H2O.UniRule annotation

Pathwayi: L-histidine biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation
Proteins known to be involved in the 9 steps of the subpathway in this organism are:
  1. ATP phosphoribosyltransferase (hisG)
  2. Histidine biosynthesis bifunctional protein HisIE (hisI)
  3. Histidine biosynthesis bifunctional protein HisIE (hisI), Phosphoribosyl-AMP cyclohydrolase (hisI)
  4. 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA)
  5. Imidazole glycerol phosphate synthase subunit HisF (hisF), Imidazole glycerol phosphate synthase subunit HisH (hisH)
  6. Histidine biosynthesis bifunctional protein HisB (hisB)
  7. Histidinol-phosphate aminotransferase (hisC)
  8. Histidine biosynthesis bifunctional protein HisB (hisB)
  9. Histidinol dehydrogenase (hisD)
This subpathway is part of the pathway L-histidine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway L-histidine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei11UniRule annotation1
Active sitei130UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processAmino-acid biosynthesis, Histidine biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00031; UER00010.

Names & Taxonomyi

Protein namesi
Recommended name:
Imidazole glycerol phosphate synthase subunit HisFUniRule annotation (EC:4.1.3.-UniRule annotation)
Alternative name(s):
IGP synthase cyclase subunitUniRule annotation
IGP synthase subunit HisFUniRule annotation
ImGP synthase subunit HisFUniRule annotation
Short name:
IGPS subunit HisFUniRule annotation
Gene namesi
Name:hisFUniRule annotation
Ordered Locus Names:MADE_000001022225Imported
ORF Names:MADE_1012325
OrganismiAlteromonas mediterranea (strain DSM 17117 / CIP 110805 / LMG 28347 / Deep ecotype)
Taxonomic identifieri1774373 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesAlteromonadaceaeAlteromonas
Proteomesi
  • UP000001870 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001349611 – 270Imidazole glycerol phosphate synthase subunit HisFAdd BLAST270

Interactioni

Subunit structurei

Heterodimer of HisH and HisF.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB4RU62.
SMRiB4RU62.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HisA/HisF family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0S. Bacteria.
COG0107. LUCA.
KOiK02500.

Family and domain databases

CDDicd04731. HisF. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01013. HisF. 1 hit.
InterProiView protein in InterPro
IPR013785. Aldolase_TIM.
IPR006062. His_biosynth.
IPR004651. HisF.
IPR011060. RibuloseP-bd_barrel.
PfamiView protein in Pfam
PF00977. His_biosynth. 1 hit.
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR00735. hisF. 1 hit.

Sequencei

Sequence statusi: Complete.

B4RU62-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLAKRIIPCL DVRDGKVVKG VQFRNHEIIG DIVPLAEQYA KAGADELVFY
60 70 80 90 100
DITASSDARV VDKSWVSRIA QVIDIPFCVA GGIKSIEDAG RILEMGADKI
110 120 130 140 150
SINSPALNNP ELINQLHDVY GQQCVVIGID SFYNEATDQY EVYKFTGDEA
160 170 180 190 200
RTQKSQWQTI DWIKEVQSRG AGEIVLNCMN QDGVRKGYDV KQLKAVRDVC
210 220 230 240 250
EVPLIASGGA GEIAHFTDVF QQADVDGALA ASVFHKGIIN IDELKAELTS
260 270
NGVAMRKRHG LTAQAEGVNP
Length:270
Mass (Da):29,505
Last modified:September 23, 2008 - v1
Checksum:iEB52E464431C0411
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001103 Genomic DNA. Translation: AGV53799.1.
RefSeqiWP_012518919.1. NC_011138.3.

Genome annotation databases

EnsemblBacteriaiAGV53799; AGV53799; MADE_000001022225.
KEGGiamc:MADE_000001022225.

Similar proteinsi

Entry informationi

Entry nameiHIS6_ALTMD
AccessioniPrimary (citable) accession number: B4RU62
Secondary accession number(s): F2G6W7, T2DLM5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: September 23, 2008
Last modified: July 5, 2017
This is version 63 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families