Reviewed, UniProtKB/Swiss-Prot B4RU34 (SYR_ALTMD)

Names and origin

Protein names

Recommended name:
    Arginyl-tRNA synthetase
    EC=6.1.1.19
Alternative name(s):
    Arginine--tRNA ligase
      Short name=ArgRS

Gene names

Name:	argS
Ordered Locus Names:	MADE_02786

Organism

Alteromonas macleodii (strain DSM 17117 / Deep ecotype) [Complete proteome] [HAMAP]

Taxonomic identifier

314275 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Alteromonadaceae › Alteromonas

Protein attributes

Sequence length	576 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg). HAMAP MF_00123
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP arginine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 576	576	Arginyl-tRNA synthetase HAMAP MF_00123		PRO_1000095331
Regions
	Motif	121 – 131	11	"HIGH" region HAMAP MF_00123

Sequences

	Sequence		Length	Mass (Da)
	B4RU34-1 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: ED517638A3E21E08	FASTA	576	64,273
	10 20 30 40 50 60 MNIHALLVNR FTEALQEMGV ENAPVPVSRS ARPEFGEYQF NGAMALAKQL KQKPRDIAEK 70 80 90 100 110 120 IVETVKLDDI ASKLEVAGPG FINVHLNDAW LANQCELSLT DPRLGIAKSP EQNIVVDYSS 130 140 150 160 170 180 PNLAKEMHVG HLRTTIIGDA VVKVLEFLGH NVIRQNHMGD WGTQFGMLLA HLSDKLQEEV 190 200 210 220 230 240 AETALSDLED FYREAKVRFD EEEGFADRAR EYVVKLQGGD AQCLALWEKF IDVSITHSEE 250 260 270 280 290 300 VYDKLNVSLT RKDIMGESAY NDDLANVISD LKTKGLAVED QGAQVVFIPE LADKEGNPAV 310 320 330 340 350 360 YIVQKSGGGY LYATTDLAAM RYRSGKLNAD RTLILTDARQ ALHFKQTEIV GRKAGFMKEE 370 380 390 400 410 420 QTYEHCPFGM MLGSDGKPFK TRTGGTVKLV ELLDEAVERA GKLIAERDND LSEEELKEVA 430 440 450 460 470 480 RKVGIGAVKY ADLSKNRTTD YMFNWDSMLS FEGNTAPYLQ YAYTRVKSLF RKAGVDMATM 490 500 510 520 530 540 PVDIKLVEKQ EHALAVLLMQ FEEVIGMVSR EATPHVLCTY LYDVASAFMT FYEACPMLKE 550 560 570 GIEPQVRDSR LALSALVAKT LEKGLTLLGI ETLEKM

References

[1]

"Comparative genomics of two ecotypes of the marine planktonic copiotroph Alteromonas macleodii suggests alternative lifestyles associated with different kinds of particulate organic matter." Ivars-Martinez E., Martin-Cuadrado A.-B., D'Auria G., Mira A., Ferriera S., Johnson J., Friedman R., Rodriguez-Valera F. ISME J. 2:1194-1212(2008) [PubMed: 18670397] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001103 Genomic DNA. Translation: ACG67082.1.
RefSeq	YP_002127076.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6776031.
GenomeReviews	Gene locus MADE_02786 in contig CP001103_GR.
KEGG	amc:MADE_02786.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	HMGFGTM.
Family and domain databases
HAMAP	MF_00123. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR001278. Arg-tRNA-synth_Ic. IPR015945. Arg-tRNA-synth_Ic_core. IPR005148. Arg-tRNA-synth_Ic_N. IPR008909. DALR_anticod_bd. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.30.1360.70. Arg-tRNA-synth_Ic_N. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11956. Arg_tRNA-synt_1c. 1 hit.
Pfam	PF03485. Arg_tRNA_synt_N. 1 hit. PF05746. DALR_1. 1 hit. PF00750. tRNA-synt_1d. 1 hit. [Graphical view]
PRINTS	PR01038. TRNASYNTHARG.
TIGRFAMs	TIGR00456. argS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYR_ALTMD

Accession

Primary (citable) accession number: B4RU34

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 23, 2008
Last modified:	November 3, 2009
This is version 10 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)