Reviewed, UniProtKB/Swiss-Prot B4RSL8 (SYT_ALTMD)

Names and origin

Protein names

Recommended name:
    Threonyl-tRNA synthetase
    EC=6.1.1.3
Alternative name(s):
    Threonine--tRNA ligase
      Short name=ThrRS

Gene names

Name:	thrS
Ordered Locus Names:	MADE_02485

Organism

Alteromonas macleodii (strain DSM 17117 / Deep ecotype) [Complete proteome] [HAMAP]

Taxonomic identifier

314275 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Alteromonadaceae › Alteromonas

Protein attributes

Sequence length	638 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP MF_00184
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00184
Subunit structure	Homodimer By similarity. HAMAP MF_00184
Subcellular location	Cytoplasm By similarity HAMAP MF_00184.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP threonine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 638	638	Threonyl-tRNA synthetase HAMAP MF_00184		PRO_1000098539
Regions
	Region	243 – 534	292	Catalytic HAMAP MF_00184
Sites
	Metal binding	334	1	Zinc; catalytic By similarity
	Metal binding	385	1	Zinc; catalytic By similarity
	Metal binding	511	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	B4RSL8-1 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: AF396540A105F9C2	FASTA	638	72,936
	10 20 30 40 50 60 MPVITLPDGS QRAFDNPVSV LDVANDIGPG LAKATIAGKV NGELVDAVDM IDADAQLQII 70 80 90 100 110 120 TAKDEEGLEI LRHSCAHLLG HAIKQLWPNT KMAIGPVIDN GFYYDVDMEE SLTQEDLAKL 130 140 150 160 170 180 EKRMLELAKT NYNVVKNKVS WQEARDAFEA RGESYKMEIL DENISKDDRP ALYQHEEYTD 190 200 210 220 230 240 MCRGPHVPNM KFCHHFKLMK VAGAYWRGDS DNKMLQRIYG TAWADKKQLK SYLQRLEEAE 250 260 270 280 290 300 KRDHRKIGKA LNLFHWQEEA PGMVFWHNDG WSIYTELESF VRKKLREYGY DEVKGPLMMD 310 320 330 340 350 360 RSLWEKSGHW DKYAENMFTT ESEKREYAVK PMNCPGHVQI FNQGLKSYRD LPLRMAEFGC 370 380 390 400 410 420 CHRNEPSGAL HGLMRVRGFT QDDAHIFCTE EQILEEVSGC IKMVYETYAA FGFENIKVKL 430 440 450 460 470 480 STRPEKRVGA DEIWDKSEAA LAEALKANDI EFDYQPGEGA FYGPKIEFTL HDCLDRAWQC 490 500 510 520 530 540 GTVQLDFSMP GRLGSTYVAE DGERKVPVMI HRAILGSLER FIGILTEEFA GFFPLWLAPQ 550 560 570 580 590 600 QVVVMNITDK QADYASDCVK KLQDLGFRAK SDLRNEKIGF KIREHTLKRI PYMLVVGDKE 610 620 630 MEAGEVAVRS RRGDDLGKMG LEDFIAMAQQ EVVEKTIK

References

[1]

"Comparative genomics of two ecotypes of the marine planktonic copiotroph Alteromonas macleodii suggests alternative lifestyles associated with different kinds of particulate organic matter." Ivars-Martinez E., Martin-Cuadrado A.-B., D'Auria G., Mira A., Ferriera S., Johnson J., Friedman R., Rodriguez-Valera F. ISME J. 2:1194-1212(2008) [PubMed: 18670397] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001103 Genomic DNA. Translation: ACG66781.1.
RefSeq	YP_002126775.1.
3D structure databases
SMR	B4RSL8. Positions 2-638.
ModBase	Search...
Genome annotation databases
GeneID	6778716.
GenomeReviews	Gene locus MADE_02485 in contig CP001103_GR.
KEGG	amc:MADE_02485.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG352811.
OMA	LEYEIQE.
Family and domain databases
HAMAP	MF_00184_B. Thr_tRNA_synth_B. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II_cons-dom. IPR004154. Anticodon_bd. IPR012675. b-grasp_ferredoxin-like. IPR004095. TGS. IPR012676. TGS-like. IPR002320. Thr-tRNA-synth_IIa. IPR018158. Thr-tRNA-synth_IIa_cons-reg. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF02824. TGS. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00418. thrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYT_ALTMD

Accession

Primary (citable) accession number: B4RSL8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 23, 2008
Last modified:	February 9, 2010
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)