Dihydroorotase - Alteromonas macleodii (strain DSM 17117 / Deep ecotype)

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Reviewed, UniProtKB/Swiss-Prot B4RRN5 (PYRC_ALTMD)

Last modified February 9, 2010. Version 17. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names

Recommended name:
    Dihydroorotase
      Short name=DHOase
    EC=3.5.2.3

Gene names

Name:	pyrC
Ordered Locus Names:	MADE_00682

Organism

Alteromonas macleodii (strain DSM 17117 / Deep ecotype) [Complete proteome] [HAMAP]

Taxonomic identifier

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Alteromonadaceae › Alteromonas

Protein attributes

Sequence length	348 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + H₂O = N-carbamoyl-L-aspartate. HAMAP MF_00219
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_00219
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3. HAMAP MF_00219
Subunit structure	Homodimer By similarity. HAMAP MF_00219
Sequence similarities	Belongs to the DHOase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

348

Dihydroorotase HAMAP MF_00219

PRO_1000100037

Sites

Metal binding

1

Zinc 1 By similarity

Metal binding

1

Zinc 1 By similarity

Metal binding

1

Zinc 1; via carbamate group By similarity

Metal binding

1

Zinc 2; via carbamate group By similarity

Metal binding

1

Zinc 2 By similarity

Metal binding

1

Zinc 2 By similarity

Metal binding

1

Zinc 1 By similarity

Amino acid modifications

Modified residue

1

N6-carboxylysine By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

B4RRN5-1 [UniParc].

Last modified September 23, 2008. Version 1.
Checksum: FB306F64913C0E6E
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348

38,312

        10         20         30         40         50         60 
MQTLTITTPD DWHLHFRDNE MLAETVPATA RCFQRAIVMP NLVPPVVNAE MAMAYKSRIE 

        70         80         90        100        110        120 
AARPNGSSFE PLMTLFLTNQ TTPQDIIDAK QAGVTACKLY PAGATTNSDA AVKGIQALYP 

       130        140        150        160        170        180 
VFQAMQEQGL LLLIHGEVTE HHIDIFDREK VFIDTHLAPI VEAFPKLKVV FEHITTADAA 

       190        200        210        220        230        240 
SFVAGASEYV GATITPQHLL LNRNDLLVGG VRPHNYCLPV LKRNTHQQAL RDMVASGNKK 

       250        260        270        280        290        300 
FFLGTDSAPH AKHKKENACG CAGCYSAWSA IELYAEVFEQ LGAIDKLEGF ASHYGADFYG 

       310        320        330        340 
LARNTTTMTL VKESWTVPEQ VTLADGTDMV PFFAGQTLQW KLEKAFQA

References

[1]

"Comparative genomics of two ecotypes of the marine planktonic copiotroph Alteromonas macleodii suggests alternative lifestyles associated with different kinds of particulate organic matter."
Ivars-Martinez E., Martin-Cuadrado A.-B., D'Auria G., Mira A., Ferriera S., Johnson J., Friedman R., Rodriguez-Valera F.
ISME J. 2:1194-1212(2008) [PubMed: 18670397] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001103 Genomic DNA. Translation: ACG65000.1.
RefSeq	YP_002124994.1.
3D structure databases
SMR	B4RRN5. Positions 2-343.
ModBase	Search...
Genome annotation databases
GeneID	6777135.
GenomeReviews	Gene locus MADE_00682 in contig CP001103_GR.
KEGG	amc:MADE_00682.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG628648.
OMA	CLPVAKR.
Family and domain databases
HAMAP	MF_00219. PyrC_type1. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR004721. DHOdimr. IPR002195. Dihydroorotase_CS. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
PIRSF	PIRSF001237. DHOdimr. 1 hit.
TIGRFAMs	TIGR00856. pyrC_dimer. 1 hit.
PROSITE	PS00482. DIHYDROOROTASE_1. 1 hit. PS00483. DIHYDROOROTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRC_ALTMD

Accession

Primary (citable) accession number: B4RRN5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 23, 2008
Last modified:	February 9, 2010
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents