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B4RPY6

- SYI_NEIG2

UniProt

B4RPY6 - SYI_NEIG2

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Neisseria gonorrhoeae (strain NCCP11945)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei563 – 5631Aminoacyl-adenylateUniRule annotation
Binding sitei608 – 6081ATPUniRule annotation
Metal bindingi892 – 8921ZincUniRule annotation
Metal bindingi895 – 8951ZincUniRule annotation
Metal bindingi912 – 9121ZincUniRule annotation
Metal bindingi915 – 9151ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciNGON521006:GJ73-102-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:NGK_0100
OrganismiNeisseria gonorrhoeae (strain NCCP11945)
Taxonomic identifieri521006 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria
ProteomesiUP000002564: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 929929Isoleucine--tRNA ligasePRO_1000189184Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi521006.NGK_0100.

Structurei

3D structure databases

ProteinModelPortaliB4RPY6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi605 – 6095"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiLGHRVHY.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B4RPY6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTDYSKTVNL LESPFPMRGN LAKCEPAWLK SWYEQKRYQK LREIAKGRPK
60 70 80 90 100
FILHDGPPYA NGDIHIGHAV NKILKDIIIR SKTQAGFDAP YVPGWDCHGL
110 120 130 140 150
PIEVMVEKLH GKDMPKARFR ELCREYAAEQ IARQKKDFIR LGVLGDWDNP
160 170 180 190 200
YLTMDFKTEA DTVRMLGEIY KSGYLYRGAK PVQFCLDCGS SLAEAEVEYK
210 220 230 240 250
DKVSPAIDVA YPFKDTVALA AAFGLAGIEG KAFAVIWTTT PWTLPASQAV
260 270 280 290 300
SAGADVVYQL IDTPKGKLVL AKDLAEGALK RYGFSDGIAI LAETTGDKLE
310 320 330 340 350
NLHMNHPFLE RDIPMLNGEH VTTDAGTGLV HTAPAHGLED YAVCNKYGIE
360 370 380 390 400
LYNPVNAEGK YISETPRVAG MSVWEANPVI LQWPEETGNL LASSKIEHSY
410 420 430 440 450
AHCWRHKTPL IYRATGQWFV GMDKAGSDGK TLRDKAIKAV DDTEFFPPWG
460 470 480 490 500
RARLESMIEG RPDWVVSRQR YWGTPMTFFV HKETGELHPN SAELLEKVAQ
510 520 530 540 550
RIEEKGIEAW FSLDKSELLS AEDCEHYDKL PDTMDVWFDS GSTHYSVVKQ
560 570 580 590 600
REELEWPADL YLEGSDQHRG WFQSSMLTGC ASSMGRAPYK QLLTHGFVVD
610 620 630 640 650
QNGRKMSKSI GNVVAPQEVY NEFGADILRL WAASTDYSGE LAISKEILKR
660 670 680 690 700
VTESYRRIRN TLSFLFANLS DFNPIEDAVP QAEMVEIDRY ALVLARRLQE
710 720 730 740 750
RLAGGYYPRY AFHFAVKDIV SFCSEDLGAF YLDILKDRLY TTKADSRARR
760 770 780 790 800
SAQTALYHIT RSLVLLIAPI LCFTGEEAWD IIGGGEEDSV LFHTWHEFPA
810 820 830 840 850
INEKAEAELV KKWTAIREAR EAVTAAIEPL RADKTVGSSL QAEAEITAPE
860 870 880 890 900
EMAGYLNALG EELRFALLVS KAEVKVGDEL AVAAKASDGE KCERCWHYTR
910 920
DVGAVAGYET VCKRCAENVG GEGETRHYA
Length:929
Mass (Da):104,155
Last modified:September 23, 2008 - v1
Checksum:iAB0495DF16BFFBB9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001050 Genomic DNA. Translation: ACF28799.1.
RefSeqiWP_012503353.1. NC_011035.1.
YP_002000725.1. NC_011035.1.

Genome annotation databases

EnsemblBacteriaiACF28799; ACF28799; NGK_0100.
GeneIDi6449599.
KEGGingk:NGK_0100.
PATRICi20338450. VBINeiGon87511_0110.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001050 Genomic DNA. Translation: ACF28799.1 .
RefSeqi WP_012503353.1. NC_011035.1.
YP_002000725.1. NC_011035.1.

3D structure databases

ProteinModelPortali B4RPY6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 521006.NGK_0100.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACF28799 ; ACF28799 ; NGK_0100 .
GeneIDi 6449599.
KEGGi ngk:NGK_0100.
PATRICi 20338450. VBINeiGon87511_0110.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi LGHRVHY.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci NGON521006:GJ73-102-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of Neisseria gonorrhoeae NCCP11945."
    Chung G.T., Yoo J.S., Oh H.B., Lee Y.S., Cha S.H., Kim S.J., Yoo C.K.
    J. Bacteriol. 190:6035-6036(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NCCP11945.

Entry informationi

Entry nameiSYI_NEIG2
AccessioniPrimary (citable) accession number: B4RPY6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: September 23, 2008
Last modified: October 29, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3