Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

B4RNT1 (SYL_NEIG2) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:NGK_0009
OrganismNeisseria gonorrhoeae (strain NCCP11945) [Complete proteome] [HAMAP]
Taxonomic identifier521006 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria

Protein attributes

Sequence length876 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 876876Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000091338

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif634 – 6385"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6371ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B4RNT1 [UniParc].

Last modified September 23, 2008. Version 1.
Checksum: 91B54A2EC262794B

FASTA87698,035
        10         20         30         40         50         60 
MQEHYQPAAI EPAAQKKWDD ARISNVSEDA SKPKYYCLSM FPYPSGKLHM GHVRNYTIGD 

        70         80         90        100        110        120 
VLSRFKLLNG FNVMQPMGWD AFGMPAENAA MKNNVAPAAW TYDNIEYMKT QLKSLGFAVD 

       130        140        150        160        170        180 
WEREVATCKP EYYRWEQWLF TKLFEKGIVY RKNGTVNWDP VDQTVLANEQ VIDGRGWRSG 

       190        200        210        220        230        240 
ALIEKREIPM YYFKITDYAE ELLNDLDKLE HWPEQVKTMQ RNWIGKSRGM TVRFAVSDDS 

       250        260        270        280        290        300 
KQGLEGDYAK FLQVYTTRPD TLMGATYVAV AAEHPLATAA AADKPELQAF IAECKAGSVA 

       310        320        330        340        350        360 
EADMATMEKK GVPTGRYVVN PLNGDKLEVW IANYVLWGYG DGAVMAVPAH DERDFEFAAK 

       370        380        390        400        410        420 
YNLPKKQVIA VGDNAFDANR WQEWYGDKEN GVLVNSGDLD GLDFQTAFDA VAAKLQSQGA 

       430        440        450        460        470        480 
GEPKTQYRLR DWGISRQRYW GCPIPIVHCE KCGDVPVPAD QLPVVLPENV VPDGMGSPLA 

       490        500        510        520        530        540 
KMPEFYETSC PCCGGAAKRE TDTMDTFMES SWYFFRYMSP KFSDGMVSAE SAKYWGAVDQ 

       550        560        570        580        590        600 
YIGGIEHAIL HLLYARFFTK LMRDEGLVNV DEPFERLLTQ GMVVCETYYR ENDKGGKDWI 

       610        620        630        640        650        660 
NPADVELTFD DKGRPVSAVL KADGLPVVIS GTEKMSKSKN NGVDPQELIN AYGADTARLF 

       670        680        690        700        710        720 
MMFAAPPEQS LEWSDSGVEG AHRFLRRLWR TVYEYLKQGG AVKAFAGNQD GLSKELKDLR 

       730        740        750        760        770        780 
HKLHSTTAKV SDDYGRRQQF NTAIAAVMEL LNQYDKTDTG SEQGRAVAQE VLEAAVRLLW 

       790        800        810        820        830        840 
PIVPHICETL WSELNGAKLW EAGWPTVDEA ALVKSEIEVM VQVNGKLRGK ITVAADASKA 

       850        860        870 
DLEAAALANE GAVKFMEGKP AKKIIVVPGR LVNIVV 

« Hide

References

[1]"Complete genome sequence of Neisseria gonorrhoeae NCCP11945."
Chung G.T., Yoo J.S., Oh H.B., Lee Y.S., Cha S.H., Kim S.J., Yoo C.K.
J. Bacteriol. 190:6035-6036(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NCCP11945.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001050 Genomic DNA. Translation: ACF28712.1.
RefSeqYP_002000634.1. NC_011035.1.

3D structure databases

ProteinModelPortalB4RNT1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING521006.NGK_0009.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACF28712; ACF28712; NGK_0009.
GeneID6447065.
KEGGngk:NGK_0009.
PATRIC20338262. VBINeiGon87511_0017.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAPVIHCPK.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycNGON521006:GJ73-9-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_NEIG2
AccessionPrimary (citable) accession number: B4RNT1
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 23, 2008
Last modified: May 14, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries