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B4RMJ8

- B4RMJ8_NEIG2

UniProt

B4RMJ8 - B4RMJ8_NEIG2

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Protein
Proline--tRNA ligase
Gene
proS, NGK_1358
Organism
Neisseria gonorrhoeae (strain NCCP11945)
Status
Unreviewed - Annotation score: 2 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS By similarity.UniRule annotationSAAS annotations

Catalytic activityi

ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro).UniRule annotationSAAS annotations

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. proline-tRNA ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. prolyl-tRNA aminoacylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetaseUniRule annotationSAAS annotationsImported, Ligase

Keywords - Biological processi

Protein biosynthesisUniRule annotationSAAS annotations

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotations, Nucleotide-binding

Enzyme and pathway databases

BioCyciNGON521006:GJ73-1382-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Proline--tRNA ligaseUniRule annotation (EC:6.1.1.15UniRule annotation)
Alternative name(s):
Prolyl-tRNA synthetase
Gene namesi
Name:proSUniRule annotation
Ordered Locus Names:NGK_1358Imported
OrganismiNeisseria gonorrhoeae (strain NCCP11945)Imported
Taxonomic identifieri521006 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria
ProteomesiUP000002564: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotationSAAS annotations

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotations

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotationSAAS annotations

Protein-protein interaction databases

STRINGi521006.NGK_1358.

Family & Domainsi

Domaini

Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0442.
HOGENOMiHOG000076893.
KOiK01881.
OMAiHEEIFTA.
OrthoDBiEOG6TTVMR.

Family and domain databases

Gene3Di3.40.50.800. 1 hit.
3.90.960.10. 1 hit.
HAMAPiMF_01569. Pro_tRNA_synth_type1.
InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002316. Pro-tRNA-ligase_IIa.
IPR004500. Pro-tRNA-synth_IIa_bac-type.
IPR023717. Pro-tRNA-Synthase_IIa_type1.
IPR007214. YbaK/aa-tRNA-synth-assoc-dom.
[Graphical view]
PANTHERiPTHR11451:SF3. PTHR11451:SF3. 1 hit.
PfamiPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF04073. tRNA_edit. 1 hit.
[Graphical view]
PRINTSiPR01046. TRNASYNTHPRO.
SUPFAMiSSF52954. SSF52954. 1 hit.
SSF55826. SSF55826. 1 hit.
TIGRFAMsiTIGR00409. proS_fam_II. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B4RMJ8-1 [UniParc]FASTAAdd to Basket

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MEVGAKPAFF ALQSAPDLLL NLPVWKNTFM KASQFFISTL KEAPAEAAFA    50
SHKLMIRAGL IKANASGLYT WMPMGLRVLR KVENVVREEM ARAGSVELLM 100
PVVQPAELWQ ESGRWEFYGK ELLRLKDRHE RDFCMGPTCE EVIADIVRKE 150
INSYKQLPKN FYHIQTKFRD EVRPRFGVMR AREFVMKDAY SFHADYASLQ 200
ATYDAMYDAY CRIFTRLGLA FRPVAADTGS IGGTGSHEFQ VLAESGEDVI 250
AYSDTSDYAA NIELAPTLPL KGERAAAQAV LTKVHTPNVK TIESLVEFLN 300
IPVEQTLKSI VVEGENEGEL VLLLLRGDHE FNDIKAEKLA GVKSPLTMAS 350
PAAIVEQFGA NGGSLGPVGF TGKVYADFAT EKGADWVIGA NEDDYHYTGF 400
NFGRDAAEPE FVDLRNVVEG DESPDGQGRL KLARGIEVGH VFQLRGKYTQ 450
AMNVSFLDNN GKSQIMEMGC YGIGITRVVA AAIEQNNDEK GIIWTKAMAP 500
FEVVIVPMNY KKSDTVREAA DRIYAELLAA GADVLLDDRD ERAGVLLNDS 550
ELLGIPHRIV IGDRALKEGN VEYAERRGNE AQAVAIGEIV ARVTASLNA 599
Length:599
Mass (Da):66,094
Last modified:September 23, 2008 - v1
Checksum:iD47545836798FB16
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001050 Genomic DNA. Translation: ACF30032.1.
RefSeqiYP_002001983.1. NC_011035.1.

Genome annotation databases

EnsemblBacteriaiACF30032; ACF30032; NGK_1358.
GeneIDi6447227.
KEGGingk:NGK_1358.
PATRICi20341183. VBINeiGon87511_1457.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001050 Genomic DNA. Translation: ACF30032.1 .
RefSeqi YP_002001983.1. NC_011035.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 521006.NGK_1358.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACF30032 ; ACF30032 ; NGK_1358 .
GeneIDi 6447227.
KEGGi ngk:NGK_1358.
PATRICi 20341183. VBINeiGon87511_1457.

Phylogenomic databases

eggNOGi COG0442.
HOGENOMi HOG000076893.
KOi K01881.
OMAi HEEIFTA.
OrthoDBi EOG6TTVMR.

Enzyme and pathway databases

BioCyci NGON521006:GJ73-1382-MONOMER.

Family and domain databases

Gene3Di 3.40.50.800. 1 hit.
3.90.960.10. 1 hit.
HAMAPi MF_01569. Pro_tRNA_synth_type1.
InterProi IPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002316. Pro-tRNA-ligase_IIa.
IPR004500. Pro-tRNA-synth_IIa_bac-type.
IPR023717. Pro-tRNA-Synthase_IIa_type1.
IPR007214. YbaK/aa-tRNA-synth-assoc-dom.
[Graphical view ]
PANTHERi PTHR11451:SF3. PTHR11451:SF3. 1 hit.
Pfami PF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF04073. tRNA_edit. 1 hit.
[Graphical view ]
PRINTSi PR01046. TRNASYNTHPRO.
SUPFAMi SSF52954. SSF52954. 1 hit.
SSF55826. SSF55826. 1 hit.
TIGRFAMsi TIGR00409. proS_fam_II. 1 hit.
PROSITEi PS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of Neisseria gonorrhoeae NCCP11945."
    Chung G.T., Yoo J.S., Oh H.B., Lee Y.S., Cha S.H., Kim S.J., Yoo C.K.
    J. Bacteriol. 190:6035-6036(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NCCP11945Imported.

Entry informationi

Entry nameiB4RMJ8_NEIG2
AccessioniPrimary (citable) accession number: B4RMJ8
Entry historyi
Integrated into UniProtKB/TrEMBL: September 23, 2008
Last sequence update: September 23, 2008
Last modified: May 14, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome

External Data

Dasty 3

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