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B4RMJ8

- B4RMJ8_NEIG2

UniProt

B4RMJ8 - B4RMJ8_NEIG2

Protein

Proline--tRNA ligase

Gene

proS

Organism
Neisseria gonorrhoeae (strain NCCP11945)
Status
Unreviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 48 (01 Oct 2014)
      Sequence version 1 (23 Sep 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS.UniRule annotationSAAS annotation

    Catalytic activityi

    ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro).UniRule annotationSAAS annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. proline-tRNA ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. prolyl-tRNA aminoacylation Source: InterPro

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetaseUniRule annotationSAAS annotationImported, Ligase

    Keywords - Biological processi

    Protein biosynthesisUniRule annotationSAAS annotation

    Keywords - Ligandi

    ATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciNGON521006:GJ73-1382-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proline--tRNA ligaseUniRule annotation (EC:6.1.1.15UniRule annotation)
    Alternative name(s):
    Prolyl-tRNA synthetaseUniRule annotation
    Gene namesi
    Name:proSUniRule annotation
    Ordered Locus Names:NGK_1358Imported
    OrganismiNeisseria gonorrhoeae (strain NCCP11945)Imported
    Taxonomic identifieri521006 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria
    ProteomesiUP000002564: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotationSAAS annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    CytoplasmUniRule annotationSAAS annotation

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotationSAAS annotation

    Protein-protein interaction databases

    STRINGi521006.NGK_1358.

    Family & Domainsi

    Domaini

    Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain.UniRule annotation

    Sequence similaritiesi

    Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0442.
    HOGENOMiHOG000076893.
    KOiK01881.
    OMAiHEEIFTA.
    OrthoDBiEOG6TTVMR.

    Family and domain databases

    Gene3Di3.40.50.800. 1 hit.
    3.90.960.10. 1 hit.
    HAMAPiMF_01569. Pro_tRNA_synth_type1.
    InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
    IPR006195. aa-tRNA-synth_II.
    IPR004154. Anticodon-bd.
    IPR002316. Pro-tRNA-ligase_IIa.
    IPR004500. Pro-tRNA-synth_IIa_bac-type.
    IPR023717. Pro-tRNA-Synthase_IIa_type1.
    IPR007214. YbaK/aa-tRNA-synth-assoc-dom.
    [Graphical view]
    PANTHERiPTHR11451:SF3. PTHR11451:SF3. 1 hit.
    PfamiPF03129. HGTP_anticodon. 1 hit.
    PF00587. tRNA-synt_2b. 1 hit.
    PF04073. tRNA_edit. 1 hit.
    [Graphical view]
    PRINTSiPR01046. TRNASYNTHPRO.
    SUPFAMiSSF52954. SSF52954. 1 hit.
    SSF55826. SSF55826. 1 hit.
    TIGRFAMsiTIGR00409. proS_fam_II. 1 hit.
    PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B4RMJ8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEVGAKPAFF ALQSAPDLLL NLPVWKNTFM KASQFFISTL KEAPAEAAFA    50
    SHKLMIRAGL IKANASGLYT WMPMGLRVLR KVENVVREEM ARAGSVELLM 100
    PVVQPAELWQ ESGRWEFYGK ELLRLKDRHE RDFCMGPTCE EVIADIVRKE 150
    INSYKQLPKN FYHIQTKFRD EVRPRFGVMR AREFVMKDAY SFHADYASLQ 200
    ATYDAMYDAY CRIFTRLGLA FRPVAADTGS IGGTGSHEFQ VLAESGEDVI 250
    AYSDTSDYAA NIELAPTLPL KGERAAAQAV LTKVHTPNVK TIESLVEFLN 300
    IPVEQTLKSI VVEGENEGEL VLLLLRGDHE FNDIKAEKLA GVKSPLTMAS 350
    PAAIVEQFGA NGGSLGPVGF TGKVYADFAT EKGADWVIGA NEDDYHYTGF 400
    NFGRDAAEPE FVDLRNVVEG DESPDGQGRL KLARGIEVGH VFQLRGKYTQ 450
    AMNVSFLDNN GKSQIMEMGC YGIGITRVVA AAIEQNNDEK GIIWTKAMAP 500
    FEVVIVPMNY KKSDTVREAA DRIYAELLAA GADVLLDDRD ERAGVLLNDS 550
    ELLGIPHRIV IGDRALKEGN VEYAERRGNE AQAVAIGEIV ARVTASLNA 599
    Length:599
    Mass (Da):66,094
    Last modified:September 23, 2008 - v1
    Checksum:iD47545836798FB16
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001050 Genomic DNA. Translation: ACF30032.1.
    RefSeqiYP_002001983.1. NC_011035.1.

    Genome annotation databases

    EnsemblBacteriaiACF30032; ACF30032; NGK_1358.
    GeneIDi6447227.
    KEGGingk:NGK_1358.
    PATRICi20341183. VBINeiGon87511_1457.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001050 Genomic DNA. Translation: ACF30032.1 .
    RefSeqi YP_002001983.1. NC_011035.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 521006.NGK_1358.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACF30032 ; ACF30032 ; NGK_1358 .
    GeneIDi 6447227.
    KEGGi ngk:NGK_1358.
    PATRICi 20341183. VBINeiGon87511_1457.

    Phylogenomic databases

    eggNOGi COG0442.
    HOGENOMi HOG000076893.
    KOi K01881.
    OMAi HEEIFTA.
    OrthoDBi EOG6TTVMR.

    Enzyme and pathway databases

    BioCyci NGON521006:GJ73-1382-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.800. 1 hit.
    3.90.960.10. 1 hit.
    HAMAPi MF_01569. Pro_tRNA_synth_type1.
    InterProi IPR002314. aa-tRNA-synt_IIb_cons-dom.
    IPR006195. aa-tRNA-synth_II.
    IPR004154. Anticodon-bd.
    IPR002316. Pro-tRNA-ligase_IIa.
    IPR004500. Pro-tRNA-synth_IIa_bac-type.
    IPR023717. Pro-tRNA-Synthase_IIa_type1.
    IPR007214. YbaK/aa-tRNA-synth-assoc-dom.
    [Graphical view ]
    PANTHERi PTHR11451:SF3. PTHR11451:SF3. 1 hit.
    Pfami PF03129. HGTP_anticodon. 1 hit.
    PF00587. tRNA-synt_2b. 1 hit.
    PF04073. tRNA_edit. 1 hit.
    [Graphical view ]
    PRINTSi PR01046. TRNASYNTHPRO.
    SUPFAMi SSF52954. SSF52954. 1 hit.
    SSF55826. SSF55826. 1 hit.
    TIGRFAMsi TIGR00409. proS_fam_II. 1 hit.
    PROSITEi PS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of Neisseria gonorrhoeae NCCP11945."
      Chung G.T., Yoo J.S., Oh H.B., Lee Y.S., Cha S.H., Kim S.J., Yoo C.K.
      J. Bacteriol. 190:6035-6036(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NCCP11945Imported.

    Entry informationi

    Entry nameiB4RMJ8_NEIG2
    AccessioniPrimary (citable) accession number: B4RMJ8
    Entry historyi
    Integrated into UniProtKB/TrEMBL: September 23, 2008
    Last sequence update: September 23, 2008
    Last modified: October 1, 2014
    This is version 48 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteomeImported

    External Data

    Dasty 3