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B4RLK9

- LIPA_NEIG2

UniProt

B4RLK9 - LIPA_NEIG2

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Protein
Lipoyl synthase
Gene
lipA, NGK_1019
Organism
Neisseria gonorrhoeae (strain NCCP11945)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi66 – 661Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi71 – 711Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi77 – 771Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi92 – 921Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi96 – 961Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi99 – 991Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciNGON521006:GJ73-1043-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:NGK_1019
OrganismiNeisseria gonorrhoeae (strain NCCP11945)
Taxonomic identifieri521006 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria
ProteomesiUP000002564: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 327327Lipoyl synthaseUniRule annotation
PRO_1000099615Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi521006.NGK_1019.

Structurei

3D structure databases

ProteinModelPortaliB4RLK9.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235997.
KOiK03644.
OMAiPEEPYNT.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

B4RLK9-1 [UniParc]FASTAAdd to Basket

« Hide

MSEIKTDDPK RGIKLRGADK TARIPIKVVP LQEKLKKPEW IRAKLPSRKF    50
FEIKDILREQ KMHTVCEEAS CPNIGECFSK GTATFMIMGD ICTRRCPFCD 100
VGHGRPNMLD PDEPKNLAES VKAMNLRYVV ITSVDRDDLR DGGAQHFADC 150
IKAIRETSPN TKIEILVPDF RGRLDIALKI LAETPPDVMN HNLETHPSLY 200
RKARPGANYQ HSLDLLKRYK EMMPHIPTKS GIMVGLGETD EDVREIMRDM 250
RAHNIEMITI GQYLQPSDGH LPVLRYVTPE QFKIFEKEAY ELGFTNAAIG 300
AMVRSSYHAD EQAAEALRES HGGCGHH 327
Length:327
Mass (Da):37,047
Last modified:September 23, 2008 - v1
Checksum:i88EEE1076164C58D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001050 Genomic DNA. Translation: ACF29696.1.
RefSeqiYP_002001644.1. NC_011035.1.

Genome annotation databases

EnsemblBacteriaiACF29696; ACF29696; NGK_1019.
GeneIDi6448749.
KEGGingk:NGK_1019.
PATRICi20340486. VBINeiGon87511_1108.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001050 Genomic DNA. Translation: ACF29696.1 .
RefSeqi YP_002001644.1. NC_011035.1.

3D structure databases

ProteinModelPortali B4RLK9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 521006.NGK_1019.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACF29696 ; ACF29696 ; NGK_1019 .
GeneIDi 6448749.
KEGGi ngk:NGK_1019.
PATRICi 20340486. VBINeiGon87511_1108.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235997.
KOi K03644.
OMAi PEEPYNT.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci NGON521006:GJ73-1043-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of Neisseria gonorrhoeae NCCP11945."
    Chung G.T., Yoo J.S., Oh H.B., Lee Y.S., Cha S.H., Kim S.J., Yoo C.K.
    J. Bacteriol. 190:6035-6036(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NCCP11945.

Entry informationi

Entry nameiLIPA_NEIG2
AccessioniPrimary (citable) accession number: B4RLK9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 23, 2008
Last modified: May 14, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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