B4RLE8 (PROB_NEIG2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate 5-kinase
EC=2.7.2.11

Alternative name(s):
Gamma-glutamyl kinase
Short name=GK

Gene names

Name:	proB
Ordered Locus Names:	NGK_0958

Organism

Neisseria gonorrhoeae (strain NCCP11945) [Complete proteome] [HAMAP]

Taxonomic identifier

521006 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria › Neisseria gonorrhoeae

Protein attributes

Sequence length	295 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline By similarity. HAMAP-Rule MF_00456
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP-Rule MF_00456
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP-Rule MF_00456
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00456.
Sequence similarities	Belongs to the glutamate 5-kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	L-proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate 5-kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 295	295	Glutamate 5-kinase HAMAP-Rule MF_00456		PRO_1000125245
Regions
	Nucleotide binding	168 – 169	2	ATP By similarity
	Nucleotide binding	210 – 216	7	ATP By similarity
Sites
	Binding site	9	1	ATP By similarity
	Binding site	49	1	Substrate By similarity
	Binding site	136	1	Substrate By similarity
	Binding site	148	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	B4RLE8 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 8C32336F4865347B	FASTA	295	31,314
	10 20 30 40 50 60 MKYKRIVFKV GTSSITRSDG SLSRGKIQTI TRQLAALHRA GHELVLVSSG AVAAGFGALG 70 80 90 100 110 120 FKKRPVKIAD KQASAAVGQG LLMEEYTANL SSDGIVSAQI LLSRADFADK RRYQNAGGAL 130 140 150 160 170 180 SVLLQRRAIP IINENDTVSV EELKIGDNDT LSAQVAAMIQ ADLLVLLTDI DDLYTGNPNS 190 200 210 220 230 240 NPDAVRLDKI EHINHEIIKM AGGSGSANGT GGMLTKIKAA TIAAESGVPV YICSSLKPDS 250 260 270 280 290 LAEAAEHQAD GSFFVPRAKG LRTQKQWLAF YSESRGSVYV DEGAEHALSE QGKAC

References

[1]	"Complete genome sequence of Neisseria gonorrhoeae NCCP11945." Chung G.T., Yoo J.S., Oh H.B., Lee Y.S., Cha S.H., Kim S.J., Yoo C.K. J. Bacteriol. 190:6035-6036(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCCP11945.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001050 Genomic DNA. Translation: ACF29635.1.
RefSeq	YP_002001583.1. NC_011035.1.
3D structure databases
ProteinModelPortal	B4RLE8.
ModBase	Search...
Protein-protein interaction databases
STRING	521006.NGK_0958.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACF29635; ACF29635; NGK_0958.
GeneID	6448994.
KEGG	ngk:NGK_0958.
PATRIC	20340344. VBINeiGon87511_1039.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0263.
HOGENOM	HOG000246368.
KO	K00931.
OMA	VYTGNPK.
Enzyme and pathway databases
BioCyc	NGON521006:GJ73-980-MONOMER.
UniPathway	UPA00098; UER00359.
Family and domain databases
Gene3D	3.40.1160.10. 1 hit.
HAMAP	MF_00456. ProB.
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu/AcGlu_kinase. IPR011529. Glu_5kinase. IPR005715. Glu_5kinase/COase_Synthase. IPR019797. Glutamate_5-kinase_CS. [Graphical view]
Pfam	PF00696. AA_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SUPFAM	SSF53633. Aa_kinase. 1 hit.
TIGRFAMs	TIGR01027. proB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROB_NEIG2

Accession

Primary (citable) accession number: B4RLE8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 23, 2008
Last modified:	May 1, 2013
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program