B4RJT4 (RISB_NEIG2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6,7-dimethyl-8-ribityllumazine synthase
Short name=DMRL synthase
Short name=Lumazine synthase
EC=2.5.1.9

Alternative name(s):
Riboflavin synthase beta chain

Gene names

Name:	ribH
Ordered Locus Names:	NGK_0394

Organism

Neisseria gonorrhoeae (strain NCCP11945) [Complete proteome] [HAMAP]

Taxonomic identifier

521006 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	158 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2-butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes the condensation of 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione with L-3,4-dihydrohy-2-butanone-4-phosphate yielding 6,7-dimethyl-8-lumazine By similarity. HAMAP MF_00178
Catalytic activity	2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine. HAMAP MF_00178
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 2/2. HAMAP MF_00178
Sequence similarities	Belongs to the DMRL synthase family.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	riboflavin synthase complex Inferred from electronic annotation. Source: InterPro
Molecular function	riboflavin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 158	158	6,7-dimethyl-8-ribityllumazine synthase HAMAP MF_00178		PRO_1000098210

Sequences

	Sequence		Length	Mass (Da)
	B4RJT4 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 0CA5502CB5690093	FASTA	158	16,968
	10 20 30 40 50 60 MNTIAPNLDG KHLRIGIVQA RFTNEIGSQM LKVCCRTLQE LGVADENITV ATVPGALEIP 70 80 90 100 110 120 IALMNFASSE KFDALIAIGV VIRGETYHFE LVANESGAGI GRVALDYNIP IANAVLTTEN 130 140 150 DAQAIERIGE KASDAAKVAV ECANLVNLLL EEQFEDEE

References

[1]	"Complete genome sequence of Neisseria gonorrhoeae NCCP11945." Chung G.T., Yoo J.S., Oh H.B., Lee Y.S., Cha S.H., Kim S.J., Yoo C.K. J. Bacteriol. 190:6035-6036(2008) [PubMed: 18586945] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCCP11945.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001050 Genomic DNA. Translation: ACF29085.1.
RefSeq	YP_002001019.1. NC_011035.1.
3D structure databases
ProteinModelPortal	B4RJT4.
ModBase	Search...
Protein-protein interaction databases
STRING	B4RJT4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBNEIT00000004576; EBNEIP00000004348; EBNEIG00000004576.
GeneID	6448244.
GenomeReviews	Gene locus NGK_0394 in contig CP001050_GR.
KEGG	ngk:NGK_0394.
PATRIC	20339124. VBINeiGon87511_0439.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000020812.
HOGENOM	HBG311126.
OMA	IQGATKH.
ProtClustDB	PRK00061.
Family and domain databases
HAMAP	MF_00178. Lumazine_synth. [Tree]
InterPro	IPR002180. DMRL_synthase. [Graphical view]
Gene3D	G3DSA:3.40.50.960. DMRL_synthase. 1 hit.
KO	K00794.
PANTHER	PTHR21058. DMRL_synthase. 1 hit.
Pfam	PF00885. DMRL_synthase. 1 hit. [Graphical view]
SUPFAM	SSF52121. DMRL_synthase. 1 hit.
TIGRFAMs	TIGR00114. Lumazine-synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RISB_NEIG2

Accession

Primary (citable) accession number: B4RJT4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 23, 2008
Last modified:	January 25, 2012
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program