B4REI3 (LPXK_PHEZH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tetraacyldisaccharide 4'-kinase
EC=2.7.1.130

Alternative name(s):
Lipid A 4'-kinase

Gene names

Name:	lpxK
Ordered Locus Names:	PHZ_c0511

Organism

Phenylobacterium zucineum (strain HLK1) [Complete proteome] [HAMAP]

Taxonomic identifier

450851 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Phenylobacterium

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1-P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA) By similarity. HAMAP MF_00409
Catalytic activity	ATP + (2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate) = ADP + (2-N,3-O-bis(3-hydroxytetradecanoyl)-4-O-phosphono-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate). HAMAP MF_00409
Pathway	Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 6/6. HAMAP MF_00409
Sequence similarities	Belongs to the LpxK family.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW tetraacyldisaccharide 4'-kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	Tetraacyldisaccharide 4'-kinase HAMAP MF_00409		PRO_1000123726
Regions
	Nucleotide binding	58 – 65	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	B4REI3 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 64C6627D0EA21172	FASTA	328	34,960
	10 20 30 40 50 60 MKLATPRWWY ERDRRSMPMT RALLTPLSWA WAGVTARRIA RARPLDAGAP VICVGNLTMG 70 80 90 100 110 120 GAGKTPVVRE IARRLGGHVL SRGYGGSLAG PVRVDPAVHA SGEVGDEPLM LARDLPVWVS 130 140 150 160 170 180 RDRLAGARAA AAAGARVVVM DDGHQNPSVK KALSLVVVDG ETRNGEWPFG DGRVFPAGPM 190 200 210 220 230 240 REPLAAGLAR ADAAVILLPA DLAAPDPELV RVLSKVPVLI ARLEPEGPPP VGPQVAFAGI 250 260 270 280 290 300 GKPWKFERAL KAAGCELVEF APFPDHYAYD AAALALLADR AAQDGAGLLT TEKDWIRLPP 310 320 AWRERVRPWP VRAVFQDEAA LDAVVGGL

References

[1]

"Complete genome of Phenylobacterium zucineum - a novel facultative intracellular bacterium isolated from human erythroleukemia cell line K562." Luo Y., Xu X., Ding Z., Liu Z., Zhang B., Yan Z., Sun J., Hu S., Hu X. BMC Genomics 9:386-386(2008) [PubMed: 18700039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HLK1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000747 Genomic DNA. Translation: ACG76925.1.
RefSeq	YP_002129354.1. NC_011144.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B4REI3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6790002.
GenomeReviews	Gene locus PHZ_c0511 in contig CP000747_GR.
KEGG	pzu:PHZ_c0511.
PATRIC	22923634. VBIPheZuc44517_0911.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG663914.
OMA	VILMDDG.
ProtClustDB	CLSK889830.
Family and domain databases
HAMAP	MF_00409. LpxK. [Tree]
InterPro	IPR003758. Tetraacyldisaccharide_4-kinase. [Graphical view]
KO	K00912.
Pfam	PF02606. LpxK. 1 hit. [Graphical view]
TIGRFAMs	TIGR00682. LpxK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LPXK_PHEZH

Accession

Primary (citable) accession number: B4REI3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 23, 2008
Last modified:	January 25, 2012
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program