Reviewed, UniProtKB/Swiss-Prot B4REI0 (PUR2_PHEZH)

Names and origin

Protein names

Recommended name:
    Phosphoribosylamine--glycine ligase
    EC=6.3.4.13
Alternative name(s):
    GARS
    Glycinamide ribonucleotide synthetase
    Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	PHZ_c0508

Organism

Phenylobacterium zucineum (strain HLK1) [Complete proteome] [HAMAP]

Taxonomic identifier

450851 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Phenylobacterium

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP MF_00138
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 423	423	Phosphoribosylamine--glycine ligase HAMAP MF_00138		PRO_1000096035
Regions
	Domain	107 – 312	206	ATP-grasp
	Nucleotide binding	133 – 193	61	ATP By similarity
Sites
	Metal binding	270	1	Magnesium or manganese 1 By similarity
	Metal binding	282	1	Magnesium or manganese 1 By similarity
	Metal binding	282	1	Magnesium or manganese 2 By similarity
	Metal binding	284	1	Magnesium or manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	B4REI0-1 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: CAD06E9B49DA13FB	FASTA	423	44,229
	10 20 30 40 50 60 MNILLVGSGG REHALAWKIA ASPLVKRLVA APGNPGMAEQ CELRAVGPTD VPALVALAKE 70 80 90 100 110 120 IAADLVVIGP ESSVDAGLAD QLQAEGIPCF GPTAGAGQLE SSKAFTKAFA DRYGLPTAAY 130 140 150 160 170 180 RVCESAEAAR AALELFEPPY VIKADGLAAG KGVTVAQDRG EAEAAIDDAF GGRFGAAGAR 190 200 210 220 230 240 VVVEEFLEGE IGSLFALCDG EASMLFGWAQ DHKRVFDGEQ GPNTGGMGTY SPAPVFTPEL 250 260 270 280 290 300 VEQVRTRLVE PAFAGIAADG SPYRGVLFVE LMATKHGPKL VEFNVRFGDP ECQVLMLRLE 310 320 330 340 350 360 SDLVPYLVAC ANGTLGDMPP PVWRDEAAVC VVIAAKGYPG TPAAGGEIRG LDQDLGDDVV 370 380 390 400 410 420 VFHAGTKRDA DGTLRAAGGR VLNVCARGAD LRTARDRAYA AIERIEFADG FCRSDIGWRA LGR

References

[1]

"Complete genome of Phenylobacterium zucineum - a novel facultative intracellular bacterium isolated from human erythroleukemia cell line K562." Luo Y., Xu X., Ding Z., Liu Z., Zhang B., Yan Z., Sun J., Hu S., Hu X. BMC Genomics 9:386-386(2008) [PubMed: 18700039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000747 Genomic DNA. Translation: ACG76922.1.
RefSeq	YP_002129351.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6789999.
GenomeReviews	Gene locus PHZ_c0508 in contig CP000747_GR.
KEGG	pzu:PHZ_c0508.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	IEYNCRF.
Family and domain databases
HAMAP	MF_00138. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N-dom. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.90.600.10. Gars. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00877. purD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_PHEZH

Accession

Primary (citable) accession number: B4REI0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 23, 2008
Last modified:	November 3, 2009
This is version 9 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)