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B4REI0 (PUR2_PHEZH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:PHZ_c0508
OrganismPhenylobacterium zucineum (strain HLK1) [Complete proteome] [HAMAP]
Taxonomic identifier450851 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaCaulobacteralesCaulobacteraceaePhenylobacterium

Protein attributes

Sequence length423 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 423423Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138
PRO_1000096035

Regions

Domain107 – 312206ATP-grasp
Nucleotide binding133 – 19361ATP By similarity

Sites

Metal binding2701Magnesium or manganese 1 By similarity
Metal binding2821Magnesium or manganese 1 By similarity
Metal binding2821Magnesium or manganese 2 By similarity
Metal binding2841Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
B4REI0 [UniParc].

Last modified September 23, 2008. Version 1.
Checksum: CAD06E9B49DA13FB

FASTA42344,229
        10         20         30         40         50         60 
MNILLVGSGG REHALAWKIA ASPLVKRLVA APGNPGMAEQ CELRAVGPTD VPALVALAKE 

        70         80         90        100        110        120 
IAADLVVIGP ESSVDAGLAD QLQAEGIPCF GPTAGAGQLE SSKAFTKAFA DRYGLPTAAY 

       130        140        150        160        170        180 
RVCESAEAAR AALELFEPPY VIKADGLAAG KGVTVAQDRG EAEAAIDDAF GGRFGAAGAR 

       190        200        210        220        230        240 
VVVEEFLEGE IGSLFALCDG EASMLFGWAQ DHKRVFDGEQ GPNTGGMGTY SPAPVFTPEL 

       250        260        270        280        290        300 
VEQVRTRLVE PAFAGIAADG SPYRGVLFVE LMATKHGPKL VEFNVRFGDP ECQVLMLRLE 

       310        320        330        340        350        360 
SDLVPYLVAC ANGTLGDMPP PVWRDEAAVC VVIAAKGYPG TPAAGGEIRG LDQDLGDDVV 

       370        380        390        400        410        420 
VFHAGTKRDA DGTLRAAGGR VLNVCARGAD LRTARDRAYA AIERIEFADG FCRSDIGWRA 


LGR 

« Hide

References

[1]"Complete genome of Phenylobacterium zucineum - a novel facultative intracellular bacterium isolated from human erythroleukemia cell line K562."
Luo Y., Xu X., Ding Z., Liu Z., Zhang B., Yan Z., Sun J., Hu S., Hu X.
BMC Genomics 9:386-386(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HLK1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000747 Genomic DNA. Translation: ACG76922.1.
RefSeqYP_002129351.1. NC_011144.1.

3D structure databases

ProteinModelPortalB4REI0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING450851.PHZ_c0508.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACG76922; ACG76922; PHZ_c0508.
GeneID6789999.
KEGGpzu:PHZ_c0508.
PATRIC22923628. VBIPheZuc44517_0908.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0151.
HOGENOMHOG000033463.
KOK01945.
OMAIMMRLKT.
OrthoDBEOG69SKD1.

Enzyme and pathway databases

BioCycPZUC450851:GHUG-513-MONOMER.
UniPathwayUPA00074; UER00125.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_PHEZH
AccessionPrimary (citable) accession number: B4REI0
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 23, 2008
Last modified: May 14, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways