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Protein

Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

Gene

trmFO

Organism
Phenylobacterium zucineum (strain HLK1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + uracil(54) in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil(54) in tRNA + FAD.UniRule annotation

Cofactori

FADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 156FADUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciPZUC450851:GHUG-1979-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOUniRule annotation (EC:2.1.1.74UniRule annotation)
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferaseUniRule annotation
Folate-dependent tRNA(M-5-U54)-methyltransferaseUniRule annotation
Gene namesi
Name:trmFOUniRule annotation
Ordered Locus Names:PHZ_c1949
OrganismiPhenylobacterium zucineum (strain HLK1)
Taxonomic identifieri450851 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaCaulobacteralesCaulobacteraceaePhenylobacterium
Proteomesi
  • UP000001868 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 466466Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOPRO_1000135890Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi450851.PHZ_c1949.

Structurei

3D structure databases

ProteinModelPortaliB4RDH0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MnmG family. TrmFO subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QXI. Bacteria.
COG1206. LUCA.
HOGENOMiHOG000252054.
KOiK04094.
OMAiDYLNCPM.
OrthoDBiEOG6J74VT.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
HAMAPiMF_01037. TrmFO.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR020595. MnmG-rel_CS.
IPR004417. TrmFO.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B4RDH0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSMNPIHVVG GGLAGSEAAW QIAEAGVPVV LHEMRPVRRT DAHHTDRLAE
60 70 80 90 100
LVCSNSFRAD DWTGNAVGLL HAEMRRLGSI IMSAGDAHQV PAGGALAVDR
110 120 130 140 150
EGFSQAVTER LEAHPLVTIA REEIAGLPPQ DWDSVILATG PLTSPALADA
160 170 180 190 200
VLKLTGEDQL SFFDAIAPIV HFESIDMDVA WRQSRYDKAG PGGDAAAYIN
210 220 230 240 250
CPMTEAQYEA FIDALLAAPK AEFKEWEHVP YFDGCLPIEV MAERGRETLR
260 270 280 290 300
HGPMKPVGLT NAHRPDEKPH AVVQLRQDNA LGTLWNMVGF QTKLKHGAQT
310 320 330 340 350
EVFRMIPGLE KAVFARLGGL HRNTFINSPR LLDGVLRLKA QPRLRFAGQV
360 370 380 390 400
TGVEGYVESA AVGLLAGRFA AAERLGRPAV APPPTTALGG LIGHITGGHL
410 420 430 440 450
EGGSGSFQPM NINYGLIPPI APPKRDAEGR RLGAKEKTRI KKRLVGERAL
460
ADLEAWLSGA QAVAAE
Length:466
Mass (Da):50,058
Last modified:September 23, 2008 - v1
Checksum:iD26D420B146A596E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000747 Genomic DNA. Translation: ACG78360.1.
RefSeqiWP_012522502.1. NC_011144.1.

Genome annotation databases

EnsemblBacteriaiACG78360; ACG78360; PHZ_c1949.
KEGGipzu:PHZ_c1949.
PATRICi22926888. VBIPheZuc44517_2513.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000747 Genomic DNA. Translation: ACG78360.1.
RefSeqiWP_012522502.1. NC_011144.1.

3D structure databases

ProteinModelPortaliB4RDH0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi450851.PHZ_c1949.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACG78360; ACG78360; PHZ_c1949.
KEGGipzu:PHZ_c1949.
PATRICi22926888. VBIPheZuc44517_2513.

Phylogenomic databases

eggNOGiENOG4107QXI. Bacteria.
COG1206. LUCA.
HOGENOMiHOG000252054.
KOiK04094.
OMAiDYLNCPM.
OrthoDBiEOG6J74VT.

Enzyme and pathway databases

BioCyciPZUC450851:GHUG-1979-MONOMER.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
HAMAPiMF_01037. TrmFO.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR020595. MnmG-rel_CS.
IPR004417. TrmFO.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome of Phenylobacterium zucineum - a novel facultative intracellular bacterium isolated from human erythroleukemia cell line K562."
    Luo Y., Xu X., Ding Z., Liu Z., Zhang B., Yan Z., Sun J., Hu S., Hu X.
    BMC Genomics 9:386-386(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HLK1.

Entry informationi

Entry nameiTRMFO_PHEZH
AccessioniPrimary (citable) accession number: B4RDH0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: September 23, 2008
Last modified: January 20, 2016
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.