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B4RCZ4 (SYL_PHEZH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:PHZ_c3517
OrganismPhenylobacterium zucineum (strain HLK1) [Complete proteome] [HAMAP]
Taxonomic identifier450851 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaCaulobacteralesCaulobacteraceaePhenylobacterium

Protein attributes

Sequence length867 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 867867Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000199218

Regions

Motif43 – 5311"HIGH" region HAMAP-Rule MF_00049
Motif627 – 6315"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6301ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B4RCZ4 [UniParc].

Last modified September 23, 2008. Version 1.
Checksum: A803FE1B482BC32A

FASTA86796,158
        10         20         30         40         50         60 
MAATRYNPKE TEPKWRKRWD EAQAFRAVEA PGKRKYYVLE MFPYPSGRLH MGHVRNYALG 

        70         80         90        100        110        120 
DVIARYKRAQ DFSVLHPMGW DAFGLPAENA AMERGVDPKA WTYDNIARMR AELKELGLSI 

       130        140        150        160        170        180 
DWSREFATCD VEYYGQQQAL FLELFERGLV YRKESVVNWD PVDQTVLANE QVVDGKGWRS 

       190        200        210        220        230        240 
GAPVEKRKLA QWFLKITQYA DQLVDDLKTL DRWPEKVRVM QENWIGRSKG ARLRFRFAGQ 

       250        260        270        280        290        300 
PPAGHEAGVE VYTTRPDTLF GASFVGVAPD HPLAQAVAAA NPEAAAFIEK CRHGAVSEAE 

       310        320        330        340        350        360 
IETAEKEGFD TGLKVKHPFD PSWELPVWIA NFILMDYGTG AIFGCPAHDQ RDLDFARKYG 

       370        380        390        400        410        420 
LPVKPVVLPP EEAQAEAIVQ KLLNEAYTGP GRIINSGFLD GLDVEAAKAA AIARIEEQGD 

       430        440        450        460        470        480 
GQGATVYRLR DWGVSRQRAW GCPIPVVHCQ ACGVVPVRRS DLPLTHPADI EFGKAGNALE 

       490        500        510        520        530        540 
RHPTWKHTTC PGCGGPATRE TDTLDTFVDS SWYFARFANP GAEAPIDKAA ADYWLPVDQY 

       550        560        570        580        590        600 
IGGVEHAVLH LLYARFITKA LADVGMLSVR EPFAGLFTQG MVTHETYRKQ SGEWVEPKEV 

       610        620        630        640        650        660 
EVTAEGKTRR ARLVGSGEPV VIGDVEKMSK SKKNTVAPEE IFDVYGVDAA RLFVLSDSPP 

       670        680        690        700        710        720 
ERDAQWSTSG VQGAWRFVNR VWDEFDASEE PVPGTEEAVT GVATDNLRRQ HAKTVKAVTE 

       730        740        750        760        770        780 
AIEGFRFNSA IAHLYSFLNV LKAERPQGRA GALAHAHRAA LRDFALLIAP FTPHLAEECW 

       790        800        810        820        830        840 
ARIGGQGLVV EAPWPTYDPA LTQDAVKVLP VQVNGKRRGE ISAPAGAEPA EVEQLVLADP 

       850        860 
EIKARLEGLT IRKIIVVKDR IVNIVAA 

« Hide

References

[1]"Complete genome of Phenylobacterium zucineum - a novel facultative intracellular bacterium isolated from human erythroleukemia cell line K562."
Luo Y., Xu X., Ding Z., Liu Z., Zhang B., Yan Z., Sun J., Hu S., Hu X.
BMC Genomics 9:386-386(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HLK1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000747 Genomic DNA. Translation: ACG79926.1.
RefSeqYP_002132355.1. NC_011144.1.

3D structure databases

ProteinModelPortalB4RCZ4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING450851.PHZ_c3517.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACG79926; ACG79926; PHZ_c3517.
GeneID6789470.
KEGGpzu:PHZ_c3517.
PATRIC22930436. VBIPheZuc44517_4272.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAPVIHCPK.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycPZUC450851:GHUG-3562-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_PHEZH
AccessionPrimary (citable) accession number: B4RCZ4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: September 23, 2008
Last modified: May 14, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries