B4RCT1 (SYD_PHEZH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--tRNA ligase
EC=6.1.1.12

Alternative name(s):
Aspartyl-tRNA synthetase
Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	PHZ_c1857

Organism

Phenylobacterium zucineum (strain HLK1) [Complete proteome] [HAMAP]

Taxonomic identifier

450851 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Phenylobacterium

Protein attributes

Sequence length	592 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044_B
Subunit structure	Homodimer By similarity. HAMAP MF_00044_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00044_B.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aspartate-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 592	592	Aspartate--tRNA ligase HAMAP MF_00044_B		PRO_1000091024

Sequences

	Sequence		Length	Mass (Da)
	B4RCT1 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: B3B8A971B3EB977E	FASTA	592	66,337
	10 20 30 40 50 60 MHLYRTHTCG QLRASDTGSK VRLSGWVHRK RDHGGLIFID LRDHYGLTQL VVSPSTPGFD 70 80 90 100 110 120 LVEHVRAESV IRVDGEVVAR SAETVNPNLP TGEIEVVVRQ VEVLSEAAEL PLPVFGEPEY 130 140 150 160 170 180 PEDIRLKYRY LDLRRETLHR NIVLRSQVIQ SIRRRMIDQG FLEFQTPILT ASSPEGARDF 190 200 210 220 230 240 LVPSRLHPTK FYALPQAPQQ FKQLLMVSGF DRYFQIAPCF RDEDLRADRS LEFYQLDVEM 250 260 270 280 290 300 SFVTQDDVFA AIEPVMHGVF TEFGEGKPVT PYPFPRIPYR EAIAKYGSDK PDLRNPIEMQ 310 320 330 340 350 360 DVSEHFRGGG FGLFARILED AKNAVWAIPG PKGGSRAFCD RMNSWAQGEG QPGLGYIFWS 370 380 390 400 410 420 DDQGGWGGPI AKNLGPEKTD GLMKALGLGQ GDAAFFVAGD PKVFYKFAGA ARTKVGTDLK 430 440 450 460 470 480 LIDEGRFEFC WIVDFPMFEW SDEEKKYDFS HNPFSMPQGE LDALLNKEPG EIVAYQYDIV 490 500 510 520 530 540 CNGHELCSGA IRNHRPDVML KAFEIAGYGP EVVEEQFGGM LNAFRHGAPP HGGLAPGIDR 550 560 570 580 590 IVMLLAGETA IREVIAFPLN QQGQDLLMNA PSEVSEKQLK ELHIRLAPPI KA

References

[1]

"Complete genome of Phenylobacterium zucineum - a novel facultative intracellular bacterium isolated from human erythroleukemia cell line K562." Luo Y., Xu X., Ding Z., Liu Z., Zhang B., Yan Z., Sun J., Hu S., Hu X. BMC Genomics 9:386-386(2008) [PubMed: 18700039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HLK1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000747 Genomic DNA. Translation: ACG78268.1.
RefSeq	YP_002130697.1. NC_011144.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B4RCT1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6791372.
GenomeReviews	Gene locus PHZ_c1857 in contig CP000747_GR.
KEGG	pzu:PHZ_c1857.
PATRIC	22926676. VBIPheZuc44517_2408.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG396032.
OMA	YQLDVEM.
ProtClustDB	PRK00476.
Family and domain databases
HAMAP	MF_00044_B. Asp_tRNA_synth_B. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:3.30.1360.30. GAD_dom. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01876.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF55261. SSF55261. 1 hit.
TIGRFAMs	TIGR00459. AspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_PHEZH

Accession

Primary (citable) accession number: B4RCT1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 23, 2008
Last modified:	January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program