B4RCG7 (B4RCG7_PHEZH) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Malate dehydrogenase HAMAP MF_01517
EC=1.1.1.37 HAMAP MF_01517

Gene names

Name:	mdh HAMAP MF_01517 EMBL ACG76566.1
Ordered Locus Names:	PHZ_c0152

Organism

Phenylobacterium zucineum (strain HLK1) [Complete proteome] [HAMAP]

Taxonomic identifier

450851 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Phenylobacterium

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP MF_01517
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP MF_01517 SAAS SAAS010945
Subunit structure	Homodimer By similarity. HAMAP MF_01517 SAAS SAAS010945
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 2 family. HAMAP MF_01517

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle HAMAP MF_01517 SAAS SAAS010945
Ligand	NAD HAMAP MF_01517 SAAS SAAS010945
Molecular function	Oxidoreductase HAMAP MF_01517
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	13 – 19	7	NAD By similarity HAMAP MF_01517
	Nucleotide binding	133 – 135	3	NAD By similarity HAMAP MF_01517
Sites
	Active site	191	1	Proton acceptor By similarity HAMAP MF_01517
	Binding site	96	1	Substrate By similarity HAMAP MF_01517
	Binding site	102	1	Substrate By similarity HAMAP MF_01517
	Binding site	109	1	NAD By similarity HAMAP MF_01517
	Binding site	116	1	NAD By similarity HAMAP MF_01517
	Binding site	135	1	Substrate By similarity HAMAP MF_01517
	Binding site	166	1	Substrate By similarity HAMAP MF_01517

Sequences

	Sequence		Length	Mass (Da)
	B4RCG7 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 7242962500431C36	FASTA	329	35,030
	10 20 30 40 50 60 MASKPPIRVA VTGAAGNIGY ALLFRIASGE MLGKDQPVIL QLLEIPAEGP QKALKGVAME 70 80 90 100 110 120 LEDCAFPLLQ DMVLTDDANV AFKDANWCLL VGSKPRGPGM ERADLLKDNG KIFIAQGKAI 130 140 150 160 170 180 DAVAADDARV AVVGNPCNTN VMIAASQAKR LTPDRFTAMV RLDENRGRAQ LAKKAGVSIN 190 200 210 220 230 240 DVSELFIYGN HSPTMFADFT HAKIGGKAAA DVINDRAWLE NEYLPTVGKR GAAIIEARGQ 250 260 270 280 290 300 SSAASAANAL IDHVRSLNTV GEVHSIAVNS EGRYGFAEGV WAGMPVKTTA PGAYEVVTSY 310 320 EMDDFAKSKI KITNDELVAE RDTVKDMLG

References

[1]

"Complete genome of Phenylobacterium zucineum - a novel facultative intracellular bacterium isolated from human erythroleukemia cell line K562." Luo Y., Xu X., Ding Z., Liu Z., Zhang B., Yan Z., Sun J., Hu S., Hu X. BMC Genomics 9:386-386(2008) [PubMed: 18700039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000747 Genomic DNA. Translation: ACG76566.1.
RefSeq	YP_002128995.1. NC_011144.1.
3D structure databases
ProteinModelPortal	B4RCG7.
ModBase	Search...
Protein-protein interaction databases
STRING	B4RCG7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6789640.
GenomeReviews	Gene locus PHZ_c0152 in contig CP000747_GR.
KEGG	pzu:PHZ_c0152.
PATRIC	22922828. VBIPheZuc44517_0511.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG289884.
OMA	NKIQISL.
ProtClustDB	PRK05442.
Family and domain databases
HAMAP	MF_01517. Malate_dehydrog_2. [Tree]
InterPro	IPR001557. L-lactate/malate_DH. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR010945. Malate_DH_type2. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00024.
PANTHER	PTHR23382. MDH_SF1. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01759. MalateDH-SF1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B4RCG7_PHEZH

Accession

Primary (citable) accession number: B4RCG7

Entry history

Integrated into UniProtKB/TrEMBL:	September 23, 2008
Last sequence update:	September 23, 2008
Last modified:	December 14, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)