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Protein

Diaminopimelate epimerase

Gene

dapF

Organism
Phenylobacterium zucineum (strain HLK1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.UniRule annotation

Catalytic activityi

LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

Pathwayi: L-lysine biosynthesis via DAP pathway

This protein is involved in step 1 of the subpathway that synthesizes DL-2,6-diaminopimelate from LL-2,6-diaminopimelate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Diaminopimelate epimerase (dapF)
This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes DL-2,6-diaminopimelate from LL-2,6-diaminopimelate, the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei17SubstrateUniRule annotation1
Binding sitei50SubstrateUniRule annotation1
Binding sitei68SubstrateUniRule annotation1
Active sitei77Proton donor/acceptorUniRule annotation1
Binding sitei162SubstrateUniRule annotation1
Sitei164Important for catalytic activityUniRule annotation1
Binding sitei195SubstrateUniRule annotation1
Sitei213Important for catalytic activityUniRule annotation1
Active sitei222Proton donor/acceptorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Amino-acid biosynthesis, Lysine biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00034; UER00025.

Names & Taxonomyi

Protein namesi
Recommended name:
Diaminopimelate epimeraseUniRule annotation (EC:5.1.1.7UniRule annotation)
Short name:
DAP epimeraseUniRule annotation
Gene namesi
Name:dapFUniRule annotation
Ordered Locus Names:PHZ_c0127
OrganismiPhenylobacterium zucineum (strain HLK1)
Taxonomic identifieri450851 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaCaulobacteralesCaulobacteraceaePhenylobacterium
Proteomesi
  • UP000001868 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000992551 – 277Diaminopimelate epimeraseAdd BLAST277

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi77 ↔ 222

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

STRINGi450851.PHZ_c0127.

Structurei

3D structure databases

ProteinModelPortaliB4RCE2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni14 – 15Substrate bindingUniRule annotation2
Regioni77 – 79Substrate bindingUniRule annotation3
Regioni213 – 214Substrate bindingUniRule annotation2
Regioni223 – 224Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the diaminopimelate epimerase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105E4Z. Bacteria.
COG0253. LUCA.
HOGENOMiHOG000220466.
KOiK01778.
OMAiRFTKMQG.
OrthoDBiPOG091H01QC.

Family and domain databases

HAMAPiMF_00197. DAP_epimerase. 1 hit.
InterProiIPR018510. DAP_epimerase_AS.
IPR001653. DAP_epimerase_DapF.
[Graphical view]
PANTHERiPTHR31689. PTHR31689. 1 hit.
PfamiPF01678. DAP_epimerase. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00652. DapF. 1 hit.
PROSITEiPS01326. DAP_EPIMERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B4RCE2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAAMGRPFL KMNGLGNDFV VVETRSAPFE PTAAQVRAIA DRATGIGCDQ
60 70 80 90 100
LIAVDPAEGA DAHVRFWNAD GEEVGACGNG TRCVGWLLMQ SSGKDEAVIE
110 120 130 140 150
TRAGRLVATR AGERLVSVDM GPPRLEWNEI PLAEPHDTRA LDVVLYHHAD
160 170 180 190 200
LMAPPGCVSM GNPHVVFFVP DAEQAPAAEA GPAIEGHPLF PEHVNVGFAQ
210 220 230 240 250
VKAPDRIRLR VWERGAGLTK ACGTGACAAM VAASRRGLIG RHATMELDGG
260 270
ELFVEWRADG HVIMTGPAAV DFQGELP
Length:277
Mass (Da):29,372
Last modified:September 23, 2008 - v1
Checksum:i1EF73A28BBD0AE80
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000747 Genomic DNA. Translation: ACG76541.1.

Genome annotation databases

EnsemblBacteriaiACG76541; ACG76541; PHZ_c0127.
KEGGipzu:PHZ_c0127.
PATRICi22922770. VBIPheZuc44517_0482.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000747 Genomic DNA. Translation: ACG76541.1.

3D structure databases

ProteinModelPortaliB4RCE2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi450851.PHZ_c0127.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACG76541; ACG76541; PHZ_c0127.
KEGGipzu:PHZ_c0127.
PATRICi22922770. VBIPheZuc44517_0482.

Phylogenomic databases

eggNOGiENOG4105E4Z. Bacteria.
COG0253. LUCA.
HOGENOMiHOG000220466.
KOiK01778.
OMAiRFTKMQG.
OrthoDBiPOG091H01QC.

Enzyme and pathway databases

UniPathwayiUPA00034; UER00025.

Family and domain databases

HAMAPiMF_00197. DAP_epimerase. 1 hit.
InterProiIPR018510. DAP_epimerase_AS.
IPR001653. DAP_epimerase_DapF.
[Graphical view]
PANTHERiPTHR31689. PTHR31689. 1 hit.
PfamiPF01678. DAP_epimerase. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00652. DapF. 1 hit.
PROSITEiPS01326. DAP_EPIMERASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDAPF_PHEZH
AccessioniPrimary (citable) accession number: B4RCE2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 23, 2008
Last modified: November 2, 2016
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.