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B4RC79

- GLND_PHEZH

UniProt

B4RC79 - GLND_PHEZH

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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene
glnD, PHZ_c3463
Organism
Phenylobacterium zucineum (strain HLK1)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism By similarity.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity By similarity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. metal ion binding Source: InterPro
  4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciPZUC450851:GHUG-3508-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Short name:
UTase/UR
Alternative name(s):
Bifunctional [protein-PII] modification enzyme
Bifunctional nitrogen sensor protein
Including the following 2 domains:
[Protein-PII] uridylyltransferase (EC:2.7.7.59)
Short name:
PII uridylyltransferase
Short name:
UTase
[Protein-PII]-UMP uridylyl-removing enzyme (EC:3.1.4.-)
Short name:
UR
Gene namesi
Name:glnD
Ordered Locus Names:PHZ_c3463
OrganismiPhenylobacterium zucineum (strain HLK1)
Taxonomic identifieri450851 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaCaulobacteralesCaulobacteraceaePhenylobacterium
ProteomesiUP000001868: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 938938Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotationPRO_1000114757Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi450851.PHZ_c3463.

Structurei

3D structure databases

ProteinModelPortaliB4RC79.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini496 – 624129HDAdd
BLAST
Domaini734 – 81380ACT 1Add
BLAST
Domaini845 – 92480ACT 2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 379379UridylyltransferaseUniRule annotationAdd
BLAST
Regioni380 – 733354Uridylyl-removingUniRule annotationAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing By similarity.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.
Contains 2 ACT domains.
Contains 1 HD domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261779.
KOiK00990.
OMAiLYCLWDM.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B4RC79-1 [UniParc]FASTAAdd to Basket

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MPPRLRPTHL EYVVDGVKLR SQLSAAALEH AGDELAQRRA ALEILKNALF    50
RGRMIAKERL ENGAGGIETA RLLSGVTDEV VSALYDFTTV HVFRARNPTE 100
GERLALMAVG GYGRGTLAPF SDIDLLFVRP YKQTAHAESV IEYMLYALWD 150
LGFKVGHASR TIDECLKLSR EDFTIRTSIL EARRLTGDEK LAGELVERFR 200
KEVVKGTGAE FVAAKLKERD ERHARAGASR YMVEPNVKEG KGGLRDLNTL 250
FWIAQYLHPG ESLEKVMHLE IFDRREVRTF IQALDFLWAV RCHLHFATGR 300
PEERLSFDLQ PEIARRMGYG DRGDAPAVER FMRRYFLFAK DVGSLTRVFA 350
AKLEADRVKT APKGISRFIP GRAQKRKPLD EPGFHEVGGR LGVDPEIFEA 400
DPVNLLKLFR IADQRNLDLH PDAFTAASRA SGLITSAVRR DRHAAKVFLD 450
ILARGRDPQR TLALMNEAGV LGRFVPEFGR IVAQMQFNMY HSYTVDEHTL 500
RAVGVIADIA AGRLAEDHPL AVQTMPLIAD REALFLAMLL HDTGKGGAGG 550
QELAGARAAR QACERLGLER SKIELVAWLV EHHLVMSDYA QKRDVSDPRT 600
VADFARIVQS PERLRLLLVL TVADIRAVGP GVWNGWKGQL MRELYTATEA 650
VFRGGRGSDA AAALKRYQEN AAYDARVSLA KADPLAEPWA DAMEDAYFTA 700
FSEAEVLAHA RLAQQAGGGA AAEGRIRSEL NAAEVVVAAA DRPRLFVDLA 750
EAITAAGANV MGARVFTSRA GQALDVFYVQ DASGQPFGSH DPRALARLAE 800
TLACAARGEP VAREPRKPQD LGRTAAFAIT PAVMLDNEAS ETSTVVEASG 850
RDRPGLLAAL ARTISDAGLS ILSAHIDGYG ERAVDAFYVV DADGRKLTDA 900
RKRNALKSAL LAALTKAEAE TAQARRTNLQ RARASVAR 938
Length:938
Mass (Da):102,982
Last modified:September 23, 2008 - v1
Checksum:i4D684D54FEEEB95E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000747 Genomic DNA. Translation: ACG79872.1.
RefSeqiYP_002132301.1. NC_011144.1.

Genome annotation databases

EnsemblBacteriaiACG79872; ACG79872; PHZ_c3463.
GeneIDi6789416.
KEGGipzu:PHZ_c3463.
PATRICi22930318. VBIPheZuc44517_4213.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000747 Genomic DNA. Translation: ACG79872.1 .
RefSeqi YP_002132301.1. NC_011144.1.

3D structure databases

ProteinModelPortali B4RC79.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 450851.PHZ_c3463.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACG79872 ; ACG79872 ; PHZ_c3463 .
GeneIDi 6789416.
KEGGi pzu:PHZ_c3463.
PATRICi 22930318. VBIPheZuc44517_4213.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261779.
KOi K00990.
OMAi LYCLWDM.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci PZUC450851:GHUG-3508-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome of Phenylobacterium zucineum - a novel facultative intracellular bacterium isolated from human erythroleukemia cell line K562."
    Luo Y., Xu X., Ding Z., Liu Z., Zhang B., Yan Z., Sun J., Hu S., Hu X.
    BMC Genomics 9:386-386(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HLK1.

Entry informationi

Entry nameiGLND_PHEZH
AccessioniPrimary (citable) accession number: B4RC79
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 23, 2008
Last modified: June 11, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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