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B4RC79

- GLND_PHEZH

UniProt

B4RC79 - GLND_PHEZH

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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Phenylobacterium zucineum (strain HLK1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Magnesium.UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciPZUC450851:GHUG-3508-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:PHZ_c3463
OrganismiPhenylobacterium zucineum (strain HLK1)
Taxonomic identifieri450851 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaCaulobacteralesCaulobacteraceaePhenylobacterium
ProteomesiUP000001868: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 938938Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_1000114757Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi450851.PHZ_c3463.

Structurei

3D structure databases

ProteinModelPortaliB4RC79.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini496 – 624129HDUniRule annotationAdd
BLAST
Domaini734 – 81380ACT 1UniRule annotationAdd
BLAST
Domaini845 – 92480ACT 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 379379UridylyltransferaseAdd
BLAST
Regioni380 – 733354Uridylyl-removingAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation
Contains 2 ACT domains.UniRule annotation
Contains 1 HD domain.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261779.
KOiK00990.
OMAiLYCLWDM.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B4RC79-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPPRLRPTHL EYVVDGVKLR SQLSAAALEH AGDELAQRRA ALEILKNALF
60 70 80 90 100
RGRMIAKERL ENGAGGIETA RLLSGVTDEV VSALYDFTTV HVFRARNPTE
110 120 130 140 150
GERLALMAVG GYGRGTLAPF SDIDLLFVRP YKQTAHAESV IEYMLYALWD
160 170 180 190 200
LGFKVGHASR TIDECLKLSR EDFTIRTSIL EARRLTGDEK LAGELVERFR
210 220 230 240 250
KEVVKGTGAE FVAAKLKERD ERHARAGASR YMVEPNVKEG KGGLRDLNTL
260 270 280 290 300
FWIAQYLHPG ESLEKVMHLE IFDRREVRTF IQALDFLWAV RCHLHFATGR
310 320 330 340 350
PEERLSFDLQ PEIARRMGYG DRGDAPAVER FMRRYFLFAK DVGSLTRVFA
360 370 380 390 400
AKLEADRVKT APKGISRFIP GRAQKRKPLD EPGFHEVGGR LGVDPEIFEA
410 420 430 440 450
DPVNLLKLFR IADQRNLDLH PDAFTAASRA SGLITSAVRR DRHAAKVFLD
460 470 480 490 500
ILARGRDPQR TLALMNEAGV LGRFVPEFGR IVAQMQFNMY HSYTVDEHTL
510 520 530 540 550
RAVGVIADIA AGRLAEDHPL AVQTMPLIAD REALFLAMLL HDTGKGGAGG
560 570 580 590 600
QELAGARAAR QACERLGLER SKIELVAWLV EHHLVMSDYA QKRDVSDPRT
610 620 630 640 650
VADFARIVQS PERLRLLLVL TVADIRAVGP GVWNGWKGQL MRELYTATEA
660 670 680 690 700
VFRGGRGSDA AAALKRYQEN AAYDARVSLA KADPLAEPWA DAMEDAYFTA
710 720 730 740 750
FSEAEVLAHA RLAQQAGGGA AAEGRIRSEL NAAEVVVAAA DRPRLFVDLA
760 770 780 790 800
EAITAAGANV MGARVFTSRA GQALDVFYVQ DASGQPFGSH DPRALARLAE
810 820 830 840 850
TLACAARGEP VAREPRKPQD LGRTAAFAIT PAVMLDNEAS ETSTVVEASG
860 870 880 890 900
RDRPGLLAAL ARTISDAGLS ILSAHIDGYG ERAVDAFYVV DADGRKLTDA
910 920 930
RKRNALKSAL LAALTKAEAE TAQARRTNLQ RARASVAR
Length:938
Mass (Da):102,982
Last modified:September 23, 2008 - v1
Checksum:i4D684D54FEEEB95E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000747 Genomic DNA. Translation: ACG79872.1.
RefSeqiYP_002132301.1. NC_011144.1.

Genome annotation databases

EnsemblBacteriaiACG79872; ACG79872; PHZ_c3463.
GeneIDi6789416.
KEGGipzu:PHZ_c3463.
PATRICi22930318. VBIPheZuc44517_4213.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000747 Genomic DNA. Translation: ACG79872.1 .
RefSeqi YP_002132301.1. NC_011144.1.

3D structure databases

ProteinModelPortali B4RC79.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 450851.PHZ_c3463.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACG79872 ; ACG79872 ; PHZ_c3463 .
GeneIDi 6789416.
KEGGi pzu:PHZ_c3463.
PATRICi 22930318. VBIPheZuc44517_4213.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261779.
KOi K00990.
OMAi LYCLWDM.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci PZUC450851:GHUG-3508-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome of Phenylobacterium zucineum - a novel facultative intracellular bacterium isolated from human erythroleukemia cell line K562."
    Luo Y., Xu X., Ding Z., Liu Z., Zhang B., Yan Z., Sun J., Hu S., Hu X.
    BMC Genomics 9:386-386(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HLK1.

Entry informationi

Entry nameiGLND_PHEZH
AccessioniPrimary (citable) accession number: B4RC79
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 23, 2008
Last modified: October 29, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3