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B4R9C7

- B4R9C7_PHEZH

UniProt

B4R9C7 - B4R9C7_PHEZH

Protein

Phenylalanine--tRNA ligase beta subunit

Gene

pheT

Organism
Phenylobacterium zucineum (strain HLK1)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 48 (01 Oct 2014)
      Sequence version 1 (23 Sep 2008)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe).UniRule annotationSAAS annotation

    Cofactori

    Binds 2 magnesium ions per tetramer.UniRule annotationSAAS annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi464 – 4641MagnesiumUniRule annotation
    Metal bindingi470 – 4701Magnesium; via carbonyl oxygenUniRule annotation
    Metal bindingi473 – 4731MagnesiumUniRule annotation
    Metal bindingi474 – 4741MagnesiumUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. phenylalanine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. tRNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. phenylalanyl-tRNA aminoacylation Source: UniProtKB-HAMAP
    2. tRNA processing Source: InterPro

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetaseUniRule annotationSAAS annotationImported, Ligase

    Keywords - Biological processi

    Protein biosynthesisUniRule annotationSAAS annotation

    Keywords - Ligandi

    ATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding, RNA-binding, tRNA-bindingUniRule annotationSAAS annotation

    Enzyme and pathway databases

    BioCyciPZUC450851:GHUG-3018-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phenylalanine--tRNA ligase beta subunitUniRule annotation (EC:6.1.1.20UniRule annotation)
    Alternative name(s):
    Phenylalanyl-tRNA synthetase beta subunitUniRule annotation
    Gene namesi
    Name:pheTUniRule annotationImported
    Ordered Locus Names:PHZ_c2978Imported
    OrganismiPhenylobacterium zucineum (strain HLK1)Imported
    Taxonomic identifieri450851 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaCaulobacteralesCaulobacteraceaePhenylobacterium
    ProteomesiUP000001868: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotationSAAS annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    CytoplasmUniRule annotationSAAS annotation

    Interactioni

    Subunit structurei

    Tetramer of two alpha and two beta subunits.UniRule annotationSAAS annotation

    Protein-protein interaction databases

    STRINGi450851.PHZ_c2978.

    Structurei

    3D structure databases

    ProteinModelPortaliB4R9C7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini39 – 148110tRNA-bindingUniRule annotationAdd
    BLAST
    Domaini415 – 48672B5UniRule annotationAdd
    BLAST
    Domaini713 – 80694FDX-ACBUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily.UniRule annotation
    Contains 1 B5 domain.UniRule annotation
    Contains 1 FDX-ACB domain.UniRule annotation
    Contains 1 tRNA-binding domain.UniRule annotation
    Contains B5 domain.SAAS annotation
    Contains Bdomain.SAAS annotation
    Contains FDX-ACB domain.SAAS annotation
    Contains tRNA-binding domain.SAAS annotation

    Phylogenomic databases

    eggNOGiCOG0072.
    HOGENOMiHOG000292085.
    KOiK01890.
    OMAiIYVFPAD.
    OrthoDBiEOG6CCH1J.

    Family and domain databases

    Gene3Di2.40.50.140. 1 hit.
    3.30.56.20. 1 hit.
    3.30.70.380. 1 hit.
    3.50.40.10. 1 hit.
    HAMAPiMF_00283. Phe_tRNA_synth_beta1.
    InterProiIPR005146. B3/B4_tRNA-bd.
    IPR009061. DNA-bd_dom_put.
    IPR012340. NA-bd_OB-fold.
    IPR004532. Phe-tRNA-ligase_IIc_bsu.
    IPR020825. Phe-tRNA_synthase_B3/B4.
    IPR005121. PheS_beta_Fdx_antiC-bd.
    IPR002547. tRNA-bd_dom.
    IPR005147. tRNA_synthase_B5-dom.
    [Graphical view]
    PfamiPF03483. B3_4. 1 hit.
    PF03484. B5. 1 hit.
    PF03147. FDX-ACB. 1 hit.
    PF01588. tRNA_bind. 1 hit.
    [Graphical view]
    SMARTiSM00873. B3_4. 1 hit.
    SM00874. B5. 1 hit.
    SM00896. FDX-ACB. 1 hit.
    [Graphical view]
    SUPFAMiSSF46955. SSF46955. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF54991. SSF54991. 1 hit.
    SSF56037. SSF56037. 1 hit.
    TIGRFAMsiTIGR00472. pheT_bact. 1 hit.
    PROSITEiPS51483. B5. 1 hit.
    PS51447. FDX_ACB. 1 hit.
    PS50886. TRBD. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B4R9C7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKFTLSWLKE HLETDATVEQ VVEAMTMAGL EVESVEDPAA KLAKFTVAKI    50
    VEAVQHPNAD KLRVCQVDTK DGRLEIVCGA PNARAGLTTI YAPIGAYVPG 100
    SGITLEPRPV RGVVSNGMLC SAKELEVAEE SDGIVELPEA LPVGASAAEA 150
    FGLEAVIDFE VTPNRPDWLG VRGIARDLAA AGLGKLKPET VPQVKGSFPC 200
    PVEIRVDGTA CPVFGGRLIR GVKNGPSPEW LQKRLASVGL RPINALVDIT 250
    NFITHDRARP LHVYDRALMV GDVIEARLGK GPAEPSATDA ASPAAHKDEQ 300
    LIALDGKTYD LTPEMSIIAD ADGQRPIGLG GVMGGESTGC SETTTDVFVE 350
    CAWFDPIVTA QTGRITGINS DAQYRFARGV DPQSVVPGLE LATAMILELC 400
    GGEASEIRLA GEAPAPPAPI AFDRAYVKKL AGLDLPAQRI DAILTDLGFE 450
    VAGNAVTPPS WRRDVEGKAD LVEEVARIAG YDNLPTEPLP EVATPAGGVL 500
    TLRQRRLRDA RRSLAARGYS EAITWSFMRR AFAELFGGGQ AELVLANPIA 550
    SDLDCMRPSA LGNLIEAAAR NARRGFPDAA LFEAGPNFRG DAPGDQWTAV 600
    TALVAPHAPK SWTKTAPDPL FALKADLIAL LDELGAPQLQ VVQGQNSPWW 650
    HPGRSARLQL GPKVVVAEFG ELHPQVLKAL DAEGPMLAFE VNLDAIPEPK 700
    KKGLKTRAAL ELSPLMPLKR DFAFVVSADT PAGDLVRPIL GADKQLIADV 750
    RVFDVYQGPG VPEGQKSVAI EVTVQPREKT LTDAEIEALS ARIVAAAEKA 800
    AGAKLRG 807
    Length:807
    Mass (Da):85,673
    Last modified:September 23, 2008 - v1
    Checksum:i02CFAD09F98C0B1D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000747 Genomic DNA. Translation: ACG79387.1.
    RefSeqiWP_012523525.1. NC_011144.1.
    YP_002131816.1. NC_011144.1.

    Genome annotation databases

    EnsemblBacteriaiACG79387; ACG79387; PHZ_c2978.
    GeneIDi6788926.
    KEGGipzu:PHZ_c2978.
    PATRICi22929242. VBIPheZuc44517_3680.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000747 Genomic DNA. Translation: ACG79387.1 .
    RefSeqi WP_012523525.1. NC_011144.1.
    YP_002131816.1. NC_011144.1.

    3D structure databases

    ProteinModelPortali B4R9C7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 450851.PHZ_c2978.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACG79387 ; ACG79387 ; PHZ_c2978 .
    GeneIDi 6788926.
    KEGGi pzu:PHZ_c2978.
    PATRICi 22929242. VBIPheZuc44517_3680.

    Phylogenomic databases

    eggNOGi COG0072.
    HOGENOMi HOG000292085.
    KOi K01890.
    OMAi IYVFPAD.
    OrthoDBi EOG6CCH1J.

    Enzyme and pathway databases

    BioCyci PZUC450851:GHUG-3018-MONOMER.

    Family and domain databases

    Gene3Di 2.40.50.140. 1 hit.
    3.30.56.20. 1 hit.
    3.30.70.380. 1 hit.
    3.50.40.10. 1 hit.
    HAMAPi MF_00283. Phe_tRNA_synth_beta1.
    InterProi IPR005146. B3/B4_tRNA-bd.
    IPR009061. DNA-bd_dom_put.
    IPR012340. NA-bd_OB-fold.
    IPR004532. Phe-tRNA-ligase_IIc_bsu.
    IPR020825. Phe-tRNA_synthase_B3/B4.
    IPR005121. PheS_beta_Fdx_antiC-bd.
    IPR002547. tRNA-bd_dom.
    IPR005147. tRNA_synthase_B5-dom.
    [Graphical view ]
    Pfami PF03483. B3_4. 1 hit.
    PF03484. B5. 1 hit.
    PF03147. FDX-ACB. 1 hit.
    PF01588. tRNA_bind. 1 hit.
    [Graphical view ]
    SMARTi SM00873. B3_4. 1 hit.
    SM00874. B5. 1 hit.
    SM00896. FDX-ACB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46955. SSF46955. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF54991. SSF54991. 1 hit.
    SSF56037. SSF56037. 1 hit.
    TIGRFAMsi TIGR00472. pheT_bact. 1 hit.
    PROSITEi PS51483. B5. 1 hit.
    PS51447. FDX_ACB. 1 hit.
    PS50886. TRBD. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome of Phenylobacterium zucineum - a novel facultative intracellular bacterium isolated from human erythroleukemia cell line K562."
      Luo Y., Xu X., Ding Z., Liu Z., Zhang B., Yan Z., Sun J., Hu S., Hu X.
      BMC Genomics 9:386-386(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: HLK1Imported.

    Entry informationi

    Entry nameiB4R9C7_PHEZH
    AccessioniPrimary (citable) accession number: B4R9C7
    Entry historyi
    Integrated into UniProtKB/TrEMBL: September 23, 2008
    Last sequence update: September 23, 2008
    Last modified: October 1, 2014
    This is version 48 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteomeImported

    External Data

    Dasty 3