B4R9A3 (B4R9A3_PHEZH) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061
Short name=CMK HAMAP MF_00061
EC=2.7.1.148 HAMAP MF_00061

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase HAMAP MF_00061

Gene names

Name:	ispE HAMAP MF_00061
Ordered Locus Names:	PHZ_c1359

Organism

Phenylobacterium zucineum (strain HLK1) [Complete proteome] [HAMAP]

Taxonomic identifier

450851 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Phenylobacterium

Protein attributes

Sequence length	307 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily. HAMAP MF_00061

Ontologies

Keywords
Biological process	Isoprene biosynthesis HAMAP MF_00061
Ligand	ATP-binding HAMAP MF_00061 RuleBase RU003563 Nucleotide-binding
Molecular function	Kinase HAMAP MF_00061 RuleBase RU003563 EMBL ACG77773.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	107 – 117	11	ATP By similarity HAMAP MF_00061
Sites
	Active site	21	1	By similarity HAMAP MF_00061
	Active site	149	1	By similarity HAMAP MF_00061

Sequences

	Sequence		Length	Mass (Da)
	B4R9A3 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 8E6B5144FFF56E29	FASTA	307	31,468
	10 20 30 40 50 60 MGRRRRSSPD SRVPAVFAPA KVNLFLHVGA PEPSGYHPIC SLMTFADVGD RVSAAGADAL 70 80 90 100 110 120 DLVVDGPFAR ELAGEGDNLV LRAARALIAE ARRPVPPIAL RLEKRLPVAS GLGGGSSDAG 130 140 150 160 170 180 AALRLMREAL ALDVSDARLE AVAASLGADG AACLWGRPAL AQGRGERLSP APAMPVLDAV 190 200 210 220 230 240 LVNPGAPVST ADVYRRFDAA GAFGSVEPPA APEAFESPQE VAAWLAGQRN DLEAPALAVA 250 260 270 280 290 300 PAVGAALETL RAEPETLLAR VSGSGGTCFA LCAGDIEAES LAERLQALAP DWWVVRCRLG GPSPDAA

References

[1]

"Complete genome of Phenylobacterium zucineum - a novel facultative intracellular bacterium isolated from human erythroleukemia cell line K562." Luo Y., Xu X., Ding Z., Liu Z., Zhang B., Yan Z., Sun J., Hu S., Hu X. BMC Genomics 9:386-386(2008) [PubMed: 18700039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000747 Genomic DNA. Translation: ACG77773.1.
RefSeq	YP_002130202.1. NC_011144.1.
3D structure databases
ProteinModelPortal	B4R9A3.
ModBase	Search...
Protein-protein interaction databases
STRING	B4R9A3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6790865.
GenomeReviews	Gene locus PHZ_c1359 in contig CP000747_GR.
KEGG	pzu:PHZ_c1359.
PATRIC	22925570. VBIPheZuc44517_1864.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734593.
OMA	KINLHLG.
ProtClustDB	PRK14608.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B4R9A3_PHEZH

Accession

Primary (citable) accession number: B4R9A3

Entry history

Integrated into UniProtKB/TrEMBL:	September 23, 2008
Last sequence update:	September 23, 2008
Last modified:	December 14, 2011
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)