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B4R338

- TRMB_DROSI

UniProt

B4R338 - TRMB_DROSI

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Protein
tRNA (guanine-N(7)-)-methyltransferase
Gene
GD16394
Organism
Drosophila simulans (Fruit fly)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei79 – 791S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding sitei157 – 1571S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Active sitei160 – 1601 By similarity

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
  2. tRNA binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    RNA-binding, S-adenosyl-L-methionine, tRNA-binding

    Enzyme and pathway databases

    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferase
    tRNA(m7G46)-methyltransferase
    Gene namesi
    ORF Names:GD16394
    OrganismiDrosophila simulans (Fruit fly)
    Taxonomic identifieri7240 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000304: Chromosome X, UP000000304: Unassembled WGS sequence

    Organism-specific databases

    FlyBaseiFBgn0188011. Dsim\GD16394.

    Subcellular locationi

    Nucleus By similarity UniRule annotation

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell
    Complete GO annotation...

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 256256tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation
    PRO_0000370575Add
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni102 – 1032S-adenosyl-L-methionine binding By similarity
    Regioni137 – 1382S-adenosyl-L-methionine binding By similarity
    Regioni235 – 2373S-adenosyl-L-methionine binding By similarity

    Sequence similaritiesi

    Phylogenomic databases

    KOiK03439.
    OrthoDBiEOG7PS1GC.
    PhylomeDBiB4R338.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR025763. Trm8_euk.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B4R338-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVATGGQAQD QSQNQEPDVL NPTSAVTGLP QKRYYRQRAH SNPIADHSFD    50
    YPARPEDVDW RSMYPSIQQG QQVSFADVGC GYGGFLVTLG EMFPEKLSIG 100
    MEIRVKVSDY VVDRIAALRR RSADTGAYQN IACLRTNAMK YLPNYFSKGQ 150
    LEKMFFLYPD PHFKRAKHKW RIINQALLSE YAYVLRKGGL VYTMTDVEDL 200
    HKWIVTHMEE HPLYERLTEE EANADPITPK LYQSSEEGAK VVRNKGDHFL 250
    AIFRRL 256
    Length:256
    Mass (Da):29,414
    Last modified:September 23, 2008 - v1
    Checksum:i6A0DC68BBDC8E2B5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CM000366 Genomic DNA. Translation: EDX16892.1.
    RefSeqiXP_002105951.1. XM_002105915.1.

    Genome annotation databases

    EnsemblMetazoaiFBtr0216304; FBpp0214796; FBgn0188011.
    GeneIDi6724913.
    KEGGidsi:Dsim_GD16394.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CM000366 Genomic DNA. Translation: EDX16892.1 .
    RefSeqi XP_002105951.1. XM_002105915.1.

    3D structure databases

    ModBasei Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0216304 ; FBpp0214796 ; FBgn0188011 .
    GeneIDi 6724913.
    KEGGi dsi:Dsim_GD16394.

    Organism-specific databases

    FlyBasei FBgn0188011. Dsim\GD16394.

    Phylogenomic databases

    KOi K03439.
    OrthoDBi EOG7PS1GC.
    PhylomeDBi B4R338.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_03055. tRNA_methyltr_TrmB_euk.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR025763. Trm8_euk.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Evolution of genes and genomes on the Drosophila phylogeny."
      Drosophila 12 genomes consortium
      Nature 450:203-218(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

    Entry informationi

    Entry nameiTRMB_DROSI
    AccessioniPrimary (citable) accession number: B4R338
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 5, 2009
    Last sequence update: September 23, 2008
    Last modified: July 9, 2014
    This is version 32 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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