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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

GD16394

Organism
Drosophila simulans (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei79S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei157S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Active sitei160UniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase, tRNA-binding
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
ORF Names:GD16394
OrganismiDrosophila simulans (Fruit fly)
Taxonomic identifieri7240 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000304 Componentsi: Chromosome X, Unassembled WGS sequence

Organism-specific databases

FlyBaseiFBgn0188011. Dsim\GD16394.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003705751 – 256tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST256

Expressioni

Gene expression databases

BgeeiFBgn0188011.

Structurei

3D structure databases

SMRiB4R338.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni102 – 103S-adenosyl-L-methionine bindingUniRule annotation2
Regioni137 – 138S-adenosyl-L-methionine bindingUniRule annotation2
Regioni235 – 237S-adenosyl-L-methionine bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

OMAiIGMEIRV.
OrthoDBiEOG091G0IQ7.
PhylomeDBiB4R338.

Family and domain databases

HAMAPiMF_03055. tRNA_methyltr_TrmB_euk. 1 hit.
InterProiView protein in InterPro
IPR029063. SAM-dependent_MTases.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
PANTHERiPTHR23417. PTHR23417. 1 hit.
PTHR23417:SF20. PTHR23417:SF20. 1 hit.
PfamiView protein in Pfam
PF02390. Methyltransf_4. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiView protein in PROSITE
PS51625. SAM_MT_TRMB. 1 hit.

Sequencei

Sequence statusi: Complete.

B4R338-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVATGGQAQD QSQNQEPDVL NPTSAVTGLP QKRYYRQRAH SNPIADHSFD
60 70 80 90 100
YPARPEDVDW RSMYPSIQQG QQVSFADVGC GYGGFLVTLG EMFPEKLSIG
110 120 130 140 150
MEIRVKVSDY VVDRIAALRR RSADTGAYQN IACLRTNAMK YLPNYFSKGQ
160 170 180 190 200
LEKMFFLYPD PHFKRAKHKW RIINQALLSE YAYVLRKGGL VYTMTDVEDL
210 220 230 240 250
HKWIVTHMEE HPLYERLTEE EANADPITPK LYQSSEEGAK VVRNKGDHFL

AIFRRL
Length:256
Mass (Da):29,414
Last modified:September 23, 2008 - v1
Checksum:i6A0DC68BBDC8E2B5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CM000366 Genomic DNA. Translation: EDX16892.1.

Genome annotation databases

EnsemblMetazoaiFBtr0216304; FBpp0214796; FBgn0188011.

Similar proteinsi

Entry informationi

Entry nameiTRMB_DROSI
AccessioniPrimary (citable) accession number: B4R338
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: September 23, 2008
Last modified: August 30, 2017
This is version 45 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families