B4R0A5 (DGKH_DROSI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Diacylglycerol kinase eta
Short name=DAG kinase eta
EC=2.7.1.107

Gene names

ORF Names:

GD19954

Organism

Drosophila simulans (Fruit fly) [Complete proteome]

Taxonomic identifier

7240 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1905 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA) By similarity. UniProtKB Q86XP1
Catalytic activity	ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate. UniProtKB Q86XP1
Subcellular location	Cytoplasm By similarity UniProtKB Q86XP1.
Sequence similarities	Belongs to the eukaryotic diacylglycerol kinase family. Contains 1 DAGKc domain. Contains 1 PH domain. Contains 2 phorbol-ester/DAG-type zinc fingers. Contains 1 SAM (sterile alpha motif) domain.
Sequence caution	The sequence EDX12030.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Repeat Zinc-finger
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	activation of protein kinase C activity by G-protein coupled receptor protein signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB intracellular signal transduction Inferred from electronic annotation. Source: InterPro protein oligomerization Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	endosome Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW diacylglycerol kinase activity Inferred from sequence or structural similarity. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1905	1905	Diacylglycerol kinase eta		PRO_0000375990
Regions
	Domain	82 – 175	94	PH
	Domain	350 – 486	137	DAGKc
	Domain	1842 – 1905	64	SAM
	Zinc finger	195 – 245	51	Phorbol-ester/DAG-type 1
	Zinc finger	268 – 319	52	Phorbol-ester/DAG-type 2

Sequences

	Sequence		Length	Mass (Da)
	B4R0A5 [UniParc]. Last modified May 26, 2009. Version 2. Checksum: EB292E228AD94D66	FASTA	1,905	212,678
	10 20 30 40 50 60 MSHLKLDTLH VQRSPRGSRR SSRSSGRSSA CSSGSISPVP IIPIISISHD GDESESESEI 70 80 90 100 110 120 ETEPARLFQR RMSIKCTNNL AAIIKEGFLL KHTWSFQRWR RRYFRLKRNM LFYAKDEKCD 130 140 150 160 170 180 VFDDIDLSDL CYFECGIKNV NHSFQIITPT RSLVLCAESR REMEDWLGSL KTATAPQRPR 190 200 210 220 230 240 GDSFLIEQHD ILSNHHHWYA TSHARPTYCN VCRDALSGVT SHGLSCEVCK CKVHKRCAAK 250 260 270 280 290 300 SIANCKWTTL ASVGKDIIEQ ADGSIIMPHQ WMEGNLPVSS MCAVCKKTCG SVLRLQDWRC 310 320 330 340 350 360 LWCRATVHVA CRPQMAVACP IGPAKLSVVP PTSVHSISTD DAWDVASPKG NFSPLLVFVN 370 380 390 400 410 420 SKSGDNQGVK FLRRFKQLLN PAQVFDLIST GPSLGLRLFR HFEMFRILVC SGDGSVGWVL 430 440 450 460 470 480 SEIDRFNMHK QCQVAVMPLG TGNDLARVLG WGSSCDDDTH LPQILERYES ASTKMLDRWS 490 500 510 520 530 540 IMVFEKAIPV PKTPKMSIST EQEAMLTGMV TSANHHLRFI VETNDTQTLI SSTRNLCDTV 550 560 570 580 590 600 DDLVCRISEH HKDDEQLAVK CDILRQKLNM LLDALQEEEI GAHSGDDLIA TIRSLIARSI 610 620 630 640 650 660 PVTPGSNAYL LNPNISIEKT EKDQINSKER RNSRSLRSSE KEALQCRANS VKRAIYNVVE 670 680 690 700 710 720 HSEPGRPKRY QRKLSITPFE ALKLPTNASG ESTPCTSPLP IIPPINIISP TMETSRLTCI 730 740 750 760 770 780 SPLPDTRRDS VDENFFNSIN LPAPRQFADS RRSSGVPEVI QEIEEGANGE TVYRRSRMSL 790 800 810 820 830 840 TGGANIDDAG NRLSPCSDGG ENTPTERKVD FLRVPIHTGE PIVDPLCDYR PHEVFEQKEN 850 860 870 880 890 900 DKEKDKPVEI DMERDTCVEK DGSVPEEKLV HTCNIQVPGV VVTPNPQNVY SSASITIIDT 910 920 930 940 950 960 DAQTTTEQSS SDDLGGEASD VLSAISNEEC SVASEIFDKQ DAGQTVGDII QNMDASNFTH 970 980 990 1000 1010 1020 IDSPETSDET EAMPGESIMD DISSVLGHDI TYALQDNTLT DDTTTLCSEH VGPPKPPRKK 1030 1040 1050 1060 1070 1080 SLSALSRTQA HPRRRNSSPP RIARLARMDS DDNPQQFGFE NIVFEIDNRC DDQKMREPPR 1090 1100 1110 1120 1130 1140 YCSLAQFVEG NDIARQSFKQ LMLEQHRGED NDSEYPEHQQ TPTNKGANLL ATTSEDELST 1150 1160 1170 1180 1190 1200 QTAIKIEIQD IDATVRNLNS SMKPNTILTT STSPTKKSGH GQDISVVVRP PTPLRGDSIK 1210 1220 1230 1240 1250 1260 PTVSLLPVSS GGAMAVSMTC SGMLGVRAMN ASEIRRHSSH APGLAVREFD KDKDRRHSGF 1270 1280 1290 1300 1310 1320 NPNQLTLDPE HARFLSSSPA ASRRISCGSL FKVKNKKIAT KRSYGLFSVR FFVVAEPDFR 1330 1340 1350 1360 1370 1380 LATLALIRPL IPLPNEALPN LQTLKGSKSS LFMGSTLFGF DHLASAERDK EEKGGKDKEK 1390 1400 1410 1420 1430 1440 TPTEETNRKL PIINPLVRLP NWPNLANGGG FISKCLLANA DTLCAAVSPL MDPDETLLAG 1450 1460 1470 1480 1490 1500 YHEKCVMNNY FGIGIDAKIS LDFHNKREEH PEKCRSRARN YMWYGVLGSK QLLQKTCKNL 1510 1520 1530 1540 1550 1560 EQRVQLECDG QRIPLPELQG IVILNIPSFM GGTNFWGSST KKDDIFLPPS FDDRVLEVVA 1570 1580 1590 1600 1610 1620 VFGSVQMAAS RLINLQHHRI AQCQSVQINI LGDEEIPIQV DGEAWLQPPG MIRILHKNRV 1630 1640 1650 1660 1670 1680 QMLCRNRSLE LSLKSWQEKQ RQHSISIQRD ASSTASEHAN STDEVISERE CYVLLNFIEA 1690 1700 1710 1720 1730 1740 VSSLVKWVKF LIISHPALQH DLYEVACRAS EALESIHPQG KLLEGPSLRT KLVEVIDSSR 1750 1760 1770 1780 1790 1800 QLYDDACTLL RDRGHSLILR EDLETKLSAA LANMEMELKK CSVQKCIDGK LRAYFNVLAP 1810 1820 1830 1840 1850 1860 NEEVDGRRKS RPFWVRLRSG STAGQQQFKP PMTNTREAAN NWSVNEVVTW LETMQLSEYV 1870 1880 1890 1900 DSFLKNDIRG KELLTLGRRD LKDLGVVKVG HVKRILQAIK DLSEN

References

[1]	"Evolution of genes and genomes on the Drosophila phylogeny." Drosophila 12 genomes consortium Nature 450:203-218(2007) [PubMed: 17994087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CM000364 Genomic DNA. Translation: EDX12030.1. Sequence problems.
RefSeq	XP_002102527.1. XM_002102491.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6727134.
KEGG	dsi:Dsim_GD19954.
Organism-specific databases
FlyBase	FBgn0191437. Dsim\GD19954.
Phylogenomic databases
GeneTree	EMGT00050000007391.
OrthoDB	EOG4280GF.
Family and domain databases
InterPro	IPR000756. Diacylglycerol_kin_accessory. IPR001206. Diacylglycerol_kinase_cat_dom. IPR011993. PH_type. IPR001849. Pleckstrin_homology. IPR002219. Prot_Kinase_C-like_PE/DAG-bd. IPR001660. SAM. IPR013761. SAM/pointed. IPR021129. SAM_type1. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit. G3DSA:1.10.150.50. SAM_type. 1 hit.
KO	K00901.
Pfam	PF00130. C1_1. 2 hits. PF00609. DAGK_acc. 1 hit. PF00781. DAGK_cat. 1 hit. PF00169. PH. 1 hit. PF00536. SAM_1. 1 hit. [Graphical view]
SMART	SM00109. C1. 2 hits. SM00045. DAGKa. 1 hit. SM00046. DAGKc. 1 hit. SM00233. PH. 1 hit. SM00454. SAM. 1 hit. [Graphical view]
SUPFAM	SSF47769. SAM_homology. 1 hit.
PROSITE	PS50146. DAGK. 1 hit. PS50003. PH_DOMAIN. 1 hit. PS50105. SAM_DOMAIN. 1 hit. PS00479. ZF_DAG_PE_1. 2 hits. PS50081. ZF_DAG_PE_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DGKH_DROSI

Accession

Primary (citable) accession number: B4R0A5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 26, 2009
Last sequence update:	May 26, 2009
Last modified:	November 16, 2011
This is version 25 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project