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B4QZU1 (PDE6_DROSI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 33. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
cGMP-specific 3',5'-cyclic phosphodiesterase

EC=3.1.4.35
Gene names
Name:Pde6
ORF Names:GD20415
OrganismDrosophila simulans (Fruit fly) [Complete proteome]
Taxonomic identifier7240 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length1143 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Has a role regulating cGMP transport in Malpighian tubule principal cells By similarity. UniProtKB Q9VFI9

Catalytic activity

Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate. UniProtKB Q9VFI9

Cofactor

Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.

Subunit structure

Interacts with PrBP By similarity. UniProtKB Q9VFI9

Subcellular location

Cell membrane; Lipid-anchor; Cytoplasmic side By similarity UniProtKB Q9VFI9.

Sequence similarities

Belongs to the cyclic nucleotide phosphodiesterase family.

Contains 2 GAF domains.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11401140cGMP-specific 3',5'-cyclic phosphodiesterase UniProtKB Q9VFI9
PRO_0000363698
Propeptide1141 – 11433Removed in mature form By similarity UniProtKB Q9VFI9
PRO_0000363699

Regions

Domain272 – 424153GAF 1
Domain456 – 637182GAF 2
Compositional bias6 – 4540Ser-rich

Sites

Active site7431Proton donor By similarity
Metal binding7471Divalent metal cation 1 By similarity
Metal binding7831Divalent metal cation 1 By similarity
Metal binding7841Divalent metal cation 1 By similarity
Metal binding7841Divalent metal cation 2 By similarity
Metal binding8941Divalent metal cation 1 By similarity

Amino acid modifications

Modified residue11401Cysteine methyl ester By similarity UniProtKB Q9VFI9
Lipidation11401S-farnesyl cysteine By similarity UniProtKB Q9VFI9

Sequences

Sequence LengthMass (Da)Tools
B4QZU1 [UniParc].

Last modified September 23, 2008. Version 1.
Checksum: 33060D06C24BA337

FASTA1,143126,868
        10         20         30         40         50         60 
MHGPVSRSSS SSNMTDVSSP AGGAASPVEM TTSSSSAATT SASSSKPLTN GANKTTISTV 

        70         80         90        100        110        120 
AGGVAPGAVP GPGSGAIPAS SSSGNQVKLE HHHRQSNNNR PAATNRSSET KLRSPAGESD 

       130        140        150        160        170        180 
GASRLMTPAG SSSSPSQSPS QTQASIQTQT SQQDRLAKAS TTASQQDVDE VARLFEEKPE 

       190        200        210        220        230        240 
AFEKWLTERA PPEALSRLQE FIENRKPHKR PSVTSDLFQQ WMAASPTVQQ KSPRSLSNSS 

       250        260        270        280        290        300 
ASSLPECRRH LMDLDEGELF MELIRDVANE LDIDVLCHKI LVNVGLLTHA DRGSLFLAKG 

       310        320        330        340        350        360 
TPTNKYLVAK LFDVTQKTAL KDAVTRASAE EIIIPFGIGI AGMVAQTKQM INIKEAYKDA 

       370        380        390        400        410        420 
RFNCEIDLKT GYKTNAILCM PICNYEGDII GVAQIINKTN GCMEFDEHDV EIFRRYLTFC 

       430        440        450        460        470        480 
GIGIQNAQLF EMSVQEYRRN QILLNLARSI FEEQNNLECL VTKIMTEARE LLKCERCSVF 

       490        500        510        520        530        540 
LVDLDCCEAS HLEKIIEKPN QQATRAIKSA DSFEEKKMRN RFTVLFELGG EYQAANVSRP 

       550        560        570        580        590        600 
SVSELSSSTL AQIAQFVATT GQTVNICDVI EWVRDHNQIR AEDEIDSTQA ILCMPIMNAQ 

       610        620        630        640        650        660 
KKVIGVAQLI NKANGVPFTD SDASIFEAFA IFCGLGIHNT QMYENACKLM AKQKVALECL 

       670        680        690        700        710        720 
SYHATASQDQ TEKLTQDVIA EAESYNLYSF TFTDFELVDD DTCRAVLRMF MQCNLVSQFQ 

       730        740        750        760        770        780 
IPYDVLCRWV LSVRKNYRPV KYHNWRHALN VAQTMFAMLK TGKMERFMTD LEILGLLVAC 

       790        800        810        820        830        840 
LCHDLDHRGT NNAFQTKTES PLAILYTTST MEHHHFDQCV MILNSEGNNI FQALSPEDYR 

       850        860        870        880        890        900 
SVMKTVESAI LSTDLAMYFK KRNAFLELVE NGEFDWQGEE KKDLLCGMMM TACDVSAIAK 

       910        920        930        940        950        960 
PWEVQHKVAK LVADEFFDQG DLEKLQLNTQ PVAMMDRERK DELPKMQVGF IDVICLPLYR 

       970        980        990       1000       1010       1020 
VLCDTFPWIT PLYEGTLENR RNWQDLAEKV EMGLTWIDHD TIDKPVEEFA ACADEEIKDI 

      1030       1040       1050       1060       1070       1080 
EFTVTTLNCN QQSQHGSEDS HTPEHQRSGS RLSMKKTGAL GKAVRSKLSK TLYNSMDGSK 

      1090       1100       1110       1120       1130       1140 
PKTSLKLLES HVSEDMDDKS PTSPSQPQAS GSMGRMSASS STSSTGGQMV DKSKKRSKLC 


ALL 

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References

[1]"Evolution of genes and genomes on the Drosophila phylogeny."
Drosophila 12 genomes consortium
Nature 450:203-218(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CM000364 Genomic DNA. Translation: EDX12939.1.
RefSeqXP_002103436.1. XM_002103400.1.

3D structure databases

ProteinModelPortalB4QZU1.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0220325; FBpp0218817; FBgn0191887.
GeneID6728084.
KEGGdsi:Dsim_GD20415.

Organism-specific databases

FlyBaseFBgn0191887. Dsim\GD20415.

Phylogenomic databases

KOK13763.
OrthoDBEOG7RRF69.
PhylomeDBB4QZU1.

Family and domain databases

Gene3D1.10.1300.10. 1 hit.
3.30.450.40. 3 hits.
InterProIPR003018. GAF.
IPR029016. GAF_dom_like.
IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamPF01590. GAF. 2 hits.
PF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSPR00387. PDIESTERASE1.
SMARTSM00065. GAF. 2 hits.
SM00471. HDc. 1 hit.
[Graphical view]
SUPFAMSSF55781. SSF55781. 3 hits.
PROSITEPS00126. PDEASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePDE6_DROSI
AccessionPrimary (citable) accession number: B4QZU1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: September 23, 2008
Last modified: July 9, 2014
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase