Reviewed, UniProtKB/Swiss-Prot B4QZU1 (PDE6_DROSI)

Names and origin

Protein names

Recommended name:
    cGMP-specific 3',5'-cyclic phosphodiesterase
    EC=3.1.4.35

Gene names

Name:	Pde6
ORF Names:	GD20415

Organism

Drosophila simulans (Fruit fly)

Taxonomic identifier

7240 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1143 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Has a role regulating cGMP transport in Malpighian tubule principal cells By similarity. UniProtKB Q9VFI9
Catalytic activity	Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate. UniProtKB Q9VFI9
Subunit structure	Interacts with PrBP By similarity. UniProtKB Q9VFI9
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side By similarity. UniProtKB Q9VFI9
Sequence similarities	Belongs to the cyclic nucleotide phosphodiesterase family. Contains 2 GAF domains.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Repeat
Ligand	cGMP
Molecular function	Hydrolase
PTM	Lipoprotein Methylation Prenylation
Gene Ontology (GO)
Biological process	cGMP metabolic process Inferred from sequence or structural similarity. Source: UniProtKB signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	internal side of plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3',5'-cyclic-GMP phosphodiesterase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1140	1140	cGMP-specific 3',5'-cyclic phosphodiesterase UniProtKB Q9VFI9		PRO_0000363698
	Propeptide	1141 – 1143	3	Removed in mature form By similarity UniProtKB Q9VFI9		PRO_0000363699
Regions
	Domain	272 – 424	153	GAF 1
	Domain	456 – 637	182	GAF 2
	Compositional bias	6 – 45	40	Ser-rich
Amino acid modifications
	Modified residue	1140	1	Cysteine methyl ester By similarity UniProtKB Q9VFI9
	Lipidation	1140	1	S-farnesyl cysteine By similarity UniProtKB Q9VFI9

Sequences

	Sequence		Length	Mass (Da)
	B4QZU1-1 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 33060D06C24BA337	FASTA	1,143	126,868
	10 20 30 40 50 60 MHGPVSRSSS SSNMTDVSSP AGGAASPVEM TTSSSSAATT SASSSKPLTN GANKTTISTV 70 80 90 100 110 120 AGGVAPGAVP GPGSGAIPAS SSSGNQVKLE HHHRQSNNNR PAATNRSSET KLRSPAGESD 130 140 150 160 170 180 GASRLMTPAG SSSSPSQSPS QTQASIQTQT SQQDRLAKAS TTASQQDVDE VARLFEEKPE 190 200 210 220 230 240 AFEKWLTERA PPEALSRLQE FIENRKPHKR PSVTSDLFQQ WMAASPTVQQ KSPRSLSNSS 250 260 270 280 290 300 ASSLPECRRH LMDLDEGELF MELIRDVANE LDIDVLCHKI LVNVGLLTHA DRGSLFLAKG 310 320 330 340 350 360 TPTNKYLVAK LFDVTQKTAL KDAVTRASAE EIIIPFGIGI AGMVAQTKQM INIKEAYKDA 370 380 390 400 410 420 RFNCEIDLKT GYKTNAILCM PICNYEGDII GVAQIINKTN GCMEFDEHDV EIFRRYLTFC 430 440 450 460 470 480 GIGIQNAQLF EMSVQEYRRN QILLNLARSI FEEQNNLECL VTKIMTEARE LLKCERCSVF 490 500 510 520 530 540 LVDLDCCEAS HLEKIIEKPN QQATRAIKSA DSFEEKKMRN RFTVLFELGG EYQAANVSRP 550 560 570 580 590 600 SVSELSSSTL AQIAQFVATT GQTVNICDVI EWVRDHNQIR AEDEIDSTQA ILCMPIMNAQ 610 620 630 640 650 660 KKVIGVAQLI NKANGVPFTD SDASIFEAFA IFCGLGIHNT QMYENACKLM AKQKVALECL 670 680 690 700 710 720 SYHATASQDQ TEKLTQDVIA EAESYNLYSF TFTDFELVDD DTCRAVLRMF MQCNLVSQFQ 730 740 750 760 770 780 IPYDVLCRWV LSVRKNYRPV KYHNWRHALN VAQTMFAMLK TGKMERFMTD LEILGLLVAC 790 800 810 820 830 840 LCHDLDHRGT NNAFQTKTES PLAILYTTST MEHHHFDQCV MILNSEGNNI FQALSPEDYR 850 860 870 880 890 900 SVMKTVESAI LSTDLAMYFK KRNAFLELVE NGEFDWQGEE KKDLLCGMMM TACDVSAIAK 910 920 930 940 950 960 PWEVQHKVAK LVADEFFDQG DLEKLQLNTQ PVAMMDRERK DELPKMQVGF IDVICLPLYR 970 980 990 1000 1010 1020 VLCDTFPWIT PLYEGTLENR RNWQDLAEKV EMGLTWIDHD TIDKPVEEFA ACADEEIKDI 1030 1040 1050 1060 1070 1080 EFTVTTLNCN QQSQHGSEDS HTPEHQRSGS RLSMKKTGAL GKAVRSKLSK TLYNSMDGSK 1090 1100 1110 1120 1130 1140 PKTSLKLLES HVSEDMDDKS PTSPSQPQAS GSMGRMSASS STSSTGGQMV DKSKKRSKLC ALL

References

[1]	"Evolution of genes and genomes on the Drosophila phylogeny." Drosophila 12 genomes consortium Nature 450:203-218(2007) [PubMed: 17994087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CM000364 Genomic DNA. Translation: EDX12939.1.
RefSeq	XP_002103436.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6728084.
KEGG	dsi:Dsim_GD20415.
Organism-specific databases
FlyBase	FBgn0191887. Dsim\GD20415.
Family and domain databases
InterPro	IPR003018. GAF. IPR003607. Met-dep_phosphohydro_HD. IPR002073. PDEase. [Graphical view]
Pfam	PF01590. GAF. 2 hits. PF00233. PDEase_I. 1 hit. [Graphical view]
PRINTS	PR00387. PDIESTERASE1.
SMART	SM00065. GAF. 2 hits. SM00471. HDc. 1 hit. [Graphical view]
PROSITE	PS00126. PDEASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PDE6_DROSI

Accession

Primary (citable) accession number: B4QZU1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	September 23, 2008
Last modified:	October 13, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project