B4PSS5 (PDE6_DROYA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
cGMP-specific 3',5'-cyclic phosphodiesterase
EC=3.1.4.35

Gene names

Name:	Pde6
ORF Names:	GE26445

Organism

Drosophila yakuba (Fruit fly) [Complete proteome]

Taxonomic identifier

7245 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1149 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Has a role regulating cGMP transport in Malpighian tubule principal cells By similarity. UniProtKB Q9VFI9
Catalytic activity	Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate. UniProtKB Q9VFI9
Cofactor	Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.
Subunit structure	Interacts with PrBP By similarity. UniProtKB Q9VFI9
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side By similarity UniProtKB Q9VFI9.
Sequence similarities	Belongs to the cyclic nucleotide phosphodiesterase family. Contains 2 GAF domains.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Repeat
Ligand	Metal-binding cGMP
Molecular function	Hydrolase
PTM	Lipoprotein Methylation Prenylation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cGMP metabolic process Inferred from sequence or structural similarity. Source: UniProtKB signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3',5'-cyclic-GMP phosphodiesterase activity Inferred from sequence or structural similarity. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1146	1146	cGMP-specific 3',5'-cyclic phosphodiesterase UniProtKB Q9VFI9		PRO_0000363704
	Propeptide	1147 – 1149	3	Removed in mature form By similarity UniProtKB Q9VFI9		PRO_0000363705
Regions
	Domain	278 – 430	153	GAF 1
	Domain	462 – 643	182	GAF 2
	Compositional bias	6 – 150	145	Ser-rich
Sites
	Active site	749	1	Proton donor By similarity
	Metal binding	753	1	Divalent metal cation 1 By similarity
	Metal binding	789	1	Divalent metal cation 1 By similarity
	Metal binding	790	1	Divalent metal cation 1 By similarity
	Metal binding	790	1	Divalent metal cation 2 By similarity
	Metal binding	900	1	Divalent metal cation 1 By similarity
Amino acid modifications
	Modified residue	1146	1	Cysteine methyl ester By similarity UniProtKB Q9VFI9
	Lipidation	1146	1	S-farnesyl cysteine By similarity UniProtKB Q9VFI9

Sequences

	Sequence		Length	Mass (Da)
	B4PSS5 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 96E9219B5B84983C	FASTA	1,149	127,347
	10 20 30 40 50 60 MHGTVSRSSS SSNMTDVSSP AGGAASPVEI ATSSSTAATT SASASSSKPL TNGANKTAIS 70 80 90 100 110 120 TAAGVTPGAA PGPGCAAIPA SGSSGNQVKL EHHYRQSNNN RPAGSNRSSE TKLRSPAGES 130 140 150 160 170 180 DGASRLMTPA GSSSSPSQSP SQSQSQSQAS IQTQTSQQDR LVKAPSTTAS QQDVDEVARL 190 200 210 220 230 240 FEEKPEAFEK WLTERAPPEA LSRLQEFIEN RKPHKRPSVT SDLFQQWMAA SPTVQQKSPR 250 260 270 280 290 300 SLSNSSASSL PECRRHLMDL DEGELFMELI RDVANELDID VLCHKILVNV GLLTHADRGS 310 320 330 340 350 360 LFLAKGTPTN KYLVAKLFDV TQKTALKDAV TRASAEEIII PFGIGIAGMV AQTKQMINIK 370 380 390 400 410 420 EAYKDARFNC EIDLKTGYKT NAILCMPICN YEGDIIGVAQ IINKTNGCME FDEHDVEIFR 430 440 450 460 470 480 RYLTFCGIGI QNAQLFEMSV QEYRRNQILL NLARSIFEEQ NNLECLVTKI MTEARELLKC 490 500 510 520 530 540 ERCSVFLVDL DCCEASHLEK IIEKPNQPAT RAIKSADSFE EKKMRNRFTV LFELGGEYQA 550 560 570 580 590 600 ANVSRPSVSE LSSSTLAQIA QFVATTGQTV NICDVIEWVR DHNQIRAEDE IDSTQAILCM 610 620 630 640 650 660 PIMNAQKKVI GVAQLINKAN GVPFTESDAS IFEAFAIFCG LGIHNTQMYE NACKLMAKQK 670 680 690 700 710 720 VALECLSYHA TASQDQTEKL TQDAIAEAES YNLYSFTFTD FELVDDDTCR AVLRMFMQCN 730 740 750 760 770 780 LVSQFQIPYD VLCRWVLSVR KNYRPVKYHN WRHALNVAQT MFAMLKTGKM ERFMTDLEIL 790 800 810 820 830 840 GLLVACLCHD LDHRGTNNAF QTKTESPLAI LYTTSTMEHH HFDQCVMILN SEGNNIFQAL 850 860 870 880 890 900 SPEDYRSVMK TVESAILSTD LAMYFKKRNA FLELVENGEF DWQGEEKKDL LCGMMMTACD 910 920 930 940 950 960 VSAIAKPWEV QHKVAKLVAD EFFDQGDLEK LQLNTQPVAM MDRERKDELP KMQVGFIDVI 970 980 990 1000 1010 1020 CLPLYRVLCD TFPWITPLYE GTLENRRNWQ DLAEKVEMGL TWIDHDTIDK PVEEFAACAD 1030 1040 1050 1060 1070 1080 EEIKDIEFTV TTLNCNQQSQ HGSEDSHTPE HQRSGSRLSM KKTGALGKAV RSKLSKTLYN 1090 1100 1110 1120 1130 1140 SMDGSKPKTS LKLLESHVSE DMDDKSPTSP SQPQASGSMG RMSASSSTSS AGGQMVDKSK KRSKLCALL

References

[1]	"Evolution of genes and genomes on the Drosophila phylogeny." Drosophila 12 genomes consortium Nature 450:203-218(2007) [PubMed: 17994087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tai18E2 / Tucson 14021-0261.01.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CM000160 Genomic DNA. Translation: EDW97571.1.
RefSeq	XP_002097859.1. XM_002097823.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B4PSS5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0272963; FBpp0271455; FBgn0243466.
GeneID	6537301.
KEGG	dya:Dyak_GE26445.
Organism-specific databases
FlyBase	FBgn0243466. Dyak\GE26445.
Phylogenomic databases
GeneTree	EMGT00050000002162.
OrthoDB	EOG41RN8T.
PhylomeDB	B4PSS5.
Family and domain databases
InterPro	IPR003018. GAF. IPR003607. Metal-dep_PHydrolase_HD_dom. IPR023088. PDEase. IPR002073. PDEase_catalytic_dom. IPR023174. PDEase_CS. [Graphical view]
Gene3D	G3DSA:1.10.1300.10. PDEase_catalytic_dom. 1 hit.
KO	K13763.
Pfam	PF01590. GAF. 2 hits. PF00233. PDEase_I. 1 hit. [Graphical view]
PRINTS	PR00387. PDIESTERASE1.
SMART	SM00065. GAF. 2 hits. SM00471. HDc. 1 hit. [Graphical view]
PROSITE	PS00126. PDEASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PDE6_DROYA

Accession

Primary (citable) accession number: B4PSS5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	September 23, 2008
Last modified:	November 16, 2011
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project