B4PRE2 (DGKH_DROYA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Diacylglycerol kinase eta
Short name=DAG kinase eta
EC=2.7.1.107

Gene names

ORF Names:

GE24946

Organism

Drosophila yakuba (Fruit fly) [Complete proteome]

Taxonomic identifier

7245 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1917 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA) By similarity. UniProtKB Q86XP1
Catalytic activity	ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate. UniProtKB Q86XP1
Subcellular location	Cytoplasm By similarity UniProtKB Q86XP1.
Sequence similarities	Belongs to the eukaryotic diacylglycerol kinase family. Contains 1 DAGKc domain. Contains 1 PH domain. Contains 2 phorbol-ester/DAG-type zinc fingers. Contains 1 SAM (sterile alpha motif) domain.
Sequence caution	The sequence EDW96330.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Repeat Zinc-finger
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	activation of protein kinase C activity by G-protein coupled receptor protein signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB intracellular signal transduction Inferred from electronic annotation. Source: InterPro protein oligomerization Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	endosome Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW diacylglycerol kinase activity Inferred from sequence or structural similarity. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1917	1917	Diacylglycerol kinase eta		PRO_0000375991
Regions
	Domain	82 – 175	94	PH
	Domain	350 – 486	137	DAGKc
	Domain	1854 – 1917	64	SAM
	Zinc finger	195 – 245	51	Phorbol-ester/DAG-type 1
	Zinc finger	268 – 319	52	Phorbol-ester/DAG-type 2

Sequences

	Sequence		Length	Mass (Da)
	B4PRE2 [UniParc]. Last modified May 26, 2009. Version 2. Checksum: C373CA1DE34FDBA5	FASTA	1,917	214,006
	10 20 30 40 50 60 MSHLKLDTLH VQRSPRGSRR SSRSSGRSSA CSSGSISPVP IIPIISISHD GDESESESEI 70 80 90 100 110 120 ETEPARLFQR RMSIKCTNNL AAIIKEGFLL KHTWSFQRWR RRYFPLKRNM LFYAKDEKCD 130 140 150 160 170 180 VFDDIDLSDL CYFECGIKNV NHSFQIITPT RSLVLCAESR REMEDWLGSL KTATAPQRPR 190 200 210 220 230 240 GDSFLIEQHD ILSNHHHWYA TSHARPTYCN VCRDALSGVT SHGLSCEVCK CKVHKRCAAK 250 260 270 280 290 300 SIANCKWTTL ASVGKDIIEQ ADGSIIMPHQ WMEGNLPVSS MCAVCKKTCG SVLRLQDWRC 310 320 330 340 350 360 LWCRATVHVA CRPQMAVACP IGPAKLSVVP PTSVHSISTD DAWDVASPKG NFSPLLVFVN 370 380 390 400 410 420 SKSGDNQGVK FLRRFKQLLN PAQVFDLIST GPSLGLRLFR HFEMFRILVC SGDGSVGWVL 430 440 450 460 470 480 SEIDRFNMHK QCQVAVMPLG TGNDLARVLG WGSSCDDDTH LPQILERYES ASTKMLDRWS 490 500 510 520 530 540 IMVFEKAIPV PKTPKMSIST EQEAMLTGMV TSANHHLRFI VETSDTQTLI SSTRNLCDTV 550 560 570 580 590 600 DDLVCRISEH HKDDEQLAVK CEILRQKLNM LLDALQEEEM GAHSGDDLIA TIRSLIARSI 610 620 630 640 650 660 PVTPGSSAYL LNPNISIEKT EKDQINTKER RNSRSLRSSE KEALQCRANS VKRAIYNVVE 670 680 690 700 710 720 HSEPGRPKRY QRKLSITPFE ALKLPTTASG ESSPCTSPLP IIPPINIISP TMETSRLTCI 730 740 750 760 770 780 SPLPDTRRDS VDENFFNSIN LPAPRQFADS RRSSGVPEVI QEIEEGANGE TMYRRCRMSL 790 800 810 820 830 840 TGGANIDDAG NRLSPCSDGG ENTPTERKVD FLRVPIHTGE PIVDPLCDYR PHEVFERTYY 850 860 870 880 890 900 MTREMDKDKE KDKENDKTVE IDKEKDNCVA KEDSIPAERL VHTCNLQVPG VVVTPNTQNV 910 920 930 940 950 960 YSSASITIID TDAQTTTEQS SSDDLGGEAS DILSAISNEE CSVASEIFDK QDAGQTVGDI 970 980 990 1000 1010 1020 IQNMDASNFT HIDSPETSDE TEAMPGESIM DDISSVLGHD ITYALQDNTL TDDTTTLCSE 1030 1040 1050 1060 1070 1080 HAGPPKPPRK KSLSALSRTQ AHPRRRNSSP PRIARLARMD SDDNPQQFGF ENIVFEIDNR 1090 1100 1110 1120 1130 1140 CDDQKMREPP RYCSLAQFVE GNDIARQSFK QLMLEQQQRD GDNDTEYPEQ QQTPTNKGPN 1150 1160 1170 1180 1190 1200 SLATTSEDEL SAQTAIKIEI HDIDATVRNI NSSMKPNTIL TTSTSPTKKS GHGQDISVVV 1210 1220 1230 1240 1250 1260 RPPTPLRGDS IKPTASLMPV SSGGAMAVSM NCSGMLGVRA MNASEIRRHS SHAPGLAVRE 1270 1280 1290 1300 1310 1320 FDKDKDRRHS GFNPNQLTLD PEHARFLSSS PAASRRISCG SLFKKKNKKI ATKRSYGLFS 1330 1340 1350 1360 1370 1380 VRFFVVAEPD FRLATLALIR PLIPLPNEAL PNLQSLKGSK SSLFMGSTLF GFDHLASAEK 1390 1400 1410 1420 1430 1440 DKDEKGGKDK DKTPTEETNR KLPIINPLVR LPNWPNLANG GGFISKCLLA NADTLCAAVS 1450 1460 1470 1480 1490 1500 PLMDPDETLL AGYHEKCVMN NYFGIGIDAK ISLDFHNKRE EHPEKCRSRA RNYMWYGVLG 1510 1520 1530 1540 1550 1560 SKQLLQKTCK NLEQRVQLEC DGQRIPLPEL QGIVILNIPS FMGGTNFWGS STKKDDIFLP 1570 1580 1590 1600 1610 1620 PSFDDRVLEV VAVFGSVQMA ASRLINLQHH RIAQCQSVQI NILGDEEIPI QVDGEAWLQP 1630 1640 1650 1660 1670 1680 PGMIRILHKN RVQMLCRNRS LELSLKSWQE KQRQHSISIQ RDASSTASEH ANSTDEVISE 1690 1700 1710 1720 1730 1740 RECYVLLNFI EAVSSLVKWV KFLIISHPAL QHDLYEVACR ASEALESIHP QGKLLEGPSL 1750 1760 1770 1780 1790 1800 RTKLVEVIDS SRQLYDDACT LLRDRGHSLI LREDLETKLS AALANMEMEL KKCSVQKCID 1810 1820 1830 1840 1850 1860 GKLRAYFNVL APNEESDGRR KSRPFWVRLR SGSTAGQQAF KPPLTNTREA ASNWSVNEVV 1870 1880 1890 1900 1910 TWLETMQLSE YVDSFLKNDI RGKELLTLGR RDLKDLGVVK VGHVKRILQA IKDLSEN

References

[1]	"Evolution of genes and genomes on the Drosophila phylogeny." Drosophila 12 genomes consortium Nature 450:203-218(2007) [PubMed: 17994087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tai18E2 / Tucson 14021-0261.01.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CM000160 Genomic DNA. Translation: EDW96330.1. Sequence problems.
RefSeq	XP_002096618.1. XM_002096582.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B4PRE2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6536003.
KEGG	dya:Dyak_GE24946.
Organism-specific databases
FlyBase	FBgn0242040. Dyak\GE24946.
Phylogenomic databases
GeneTree	EMGT00050000007391.
OrthoDB	EOG4280GF.
Family and domain databases
InterPro	IPR000756. Diacylglycerol_kin_accessory. IPR001206. Diacylglycerol_kinase_cat_dom. IPR011993. PH_type. IPR001849. Pleckstrin_homology. IPR002219. Prot_Kinase_C-like_PE/DAG-bd. IPR001660. SAM. IPR013761. SAM/pointed. IPR021129. SAM_type1. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit. G3DSA:1.10.150.50. SAM_type. 1 hit.
KO	K00901.
Pfam	PF00130. C1_1. 2 hits. PF00609. DAGK_acc. 1 hit. PF00781. DAGK_cat. 1 hit. PF00169. PH. 1 hit. PF00536. SAM_1. 1 hit. [Graphical view]
SMART	SM00109. C1. 2 hits. SM00045. DAGKa. 1 hit. SM00046. DAGKc. 1 hit. SM00233. PH. 1 hit. SM00454. SAM. 1 hit. [Graphical view]
SUPFAM	SSF47769. SAM_homology. 1 hit.
PROSITE	PS50146. DAGK. 1 hit. PS50003. PH_DOMAIN. 1 hit. PS50105. SAM_DOMAIN. 1 hit. PS00479. ZF_DAG_PE_1. 2 hits. PS50081. ZF_DAG_PE_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DGKH_DROYA

Accession

Primary (citable) accession number: B4PRE2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 26, 2009
Last sequence update:	May 26, 2009
Last modified:	November 16, 2011
This is version 25 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project