Spastin - Drosophila yakuba (Fruit fly)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Spastin
EC=3.6.4.3

Gene names

Name:	spas
ORF Names:	GE23439

Organism

Drosophila yakuba (Fruit fly) [Complete proteome]

Taxonomic identifier

7245 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	758 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	ATP-dependent microtubule severing protein. Stimulates microtubule minus-end depolymerization and poleward microtubule flux in the mitotic spindle. Regulates microtubule stability in the neuromuscular junction synapse By similarity.
Catalytic activity	ATP + H₂O = ADP + phosphate.
Subunit structure	Homohexamer. The homohexamer is stabilized by ATP-binding. The homohexamer may adopt a ring conformation through which microtubules pass prior to being severed. Interacts with microtubules By similarity.
Subcellular location	Membrane; Single-pass membrane protein Potential. Cytoplasm › cytoskeleton › centrosome By similarity. Cytoplasm › cytoskeleton By similarity. Chromosome By similarity. Note: Colocalizes with cellular microtubule arrays. Localizes to chromosomes from prometaphase/metaphase to anaphase, and this requires microtubules. Localizes to discrete punctate cytoplasmic foci which may correspond to secretory vesicles By similarity.
Sequence similarities	Belongs to the AAA ATPase family. Spastin subfamily. Contains 1 MIT domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Differentiation Mitosis Neurogenesis
Cellular component	Chromosome Cytoplasm Cytoskeleton Membrane Microtubule
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Developmental protein Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW microtubule severing Inferred from sequence or structural similarity. Source: UniProtKB mitosis Inferred from electronic annotation. Source: UniProtKB-KW nervous system development Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	centrosome Inferred from sequence or structural similarity. Source: UniProtKB chromosome Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microtubule Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW microtubule binding Inferred from sequence or structural similarity. Source: UniProtKB microtubule-severing ATPase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 758

758

Spastin

PRO_0000367152

Regions

Transmembrane

115 – 134

20

Helical; Potential

Domain

232 – 308

77

MIT

Nucleotide binding

523 – 530

8

ATP Potential

Region

1 – 210

210

Required for localization to punctate cytoplasmic foci By similarity

Region

208 – 758

551

Sufficient for interaction with microtubules and microtubule severing By similarity

Region

443 – 455

13

Required for interaction with microtubules By similarity

Amino acid modifications

Modified residue

439

1

Phosphothreonine By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

B4PL32 [UniParc].

Last modified September 23, 2008. Version 1.
Checksum: 6CE1F15E93E4406D
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FASTA

758

82,761

        10         20         30         40         50         60 
MVRTKNQSSS SSASSSSTKS PIKSSSATGS SGGGVGGRQS THRSSSASNV AAVVAGGSSA 

        70         80         90        100        110        120 
AGGGSSSNRR SPGSSPDGDD DTTTTDDLTP TTCSPRSGHH HTYGGYSSSV HKQNLYVVSF 

       130        140        150        160        170        180 
PIIFLFNVLR SLIYQLFCIF RYLYGASTKV IYRPHRRDCN IEIVVQNSSK EQQQSLNHPS 

       190        200        210        220        230        240 
ELNRDSDGQE QQLSNQPQRF RPIQPLEMAA NRPGGGYSPG PGDPLLAKQK HHHRRAFEYI 

       250        260        270        280        290        300 
SKALKIDEEN EGHKELAIEL YRKGIKELED GIAVDCWSGR GDVWDRAQRL HDKMQTNLSM 

       310        320        330        340        350        360 
ARDRLHFLAL REQDLQMQRL SLKEKQNEQA PSKPQRTREP MLAGMTNEPM KLRVRSSGYG 

       370        380        390        400        410        420 
PKATTGAQPT ASGRKLTIGS KRPVNLAVAN KSQTLPRNLG SKTSVGAVQR QPAKTAATPP 

       430        440        450        460        470        480 
AVRRQFSSGR NTPPQRSRTP INNNGPSGSG ASTPVVSVKG VEQKLVQLIL DEIVEGGAKV 

       490        500        510        520        530        540 
EWTDIAGQDV AKQALQEMVI LPSVRPELFT GLRAPAKGLL LFGPPGNGKT LLARAVATEC 

       550        560        570        580        590        600 
SATFLNISAA SLTSKYVGDG EKLVRALFAV ARHMQPSIIF IDEVDSLLSE RSSSEHEASR 

       610        620        630        640        650        660 
RLKTEFLVEF DGLPGNPDGD RIVVLAATNR PQELDEAALR RFTKRVYVSL PDEQTRELLL 

       670        680        690        700        710        720 
NRLLQKQGSP LDTEALRRLA KITDGYSGSD LTALAKDAAL EPIRELNVEQ VKCLDISAMR 

       730        740        750 
AITEQDFHSS LKRIRRSVAP QSLNSYEKWS QDYGDITI

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References

[1]	"Evolution of genes and genomes on the Drosophila phylogeny." Drosophila 12 genomes consortium Nature 450:203-218(2007) [PubMed: 17994087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tai18E2 / Tucson 14021-0261.01.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CM000160 Genomic DNA. Translation: EDW99017.1.
RefSeq	XP_002099305.1. XM_002099269.1.
3D structure databases
ProteinModelPortal	B4PL32.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0269957; FBpp0268449; FBgn0240623.
GeneID	6538792.
KEGG	dya:Dyak_GE23439.
Organism-specific databases
FlyBase	FBgn0240623. Dyak\GE23439.
Phylogenomic databases
GeneTree	EMGT00050000004565.
OrthoDB	EOG44XGXW.
PhylomeDB	B4PL32.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR003960. ATPase_AAA_CS. IPR007330. MIT. [Graphical view]
KO	K13254.
Pfam	PF00004. AAA. 1 hit. PF04212. MIT. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. SM00745. MIT. 1 hit. [Graphical view]
PROSITE	PS00674. AAA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPAST_DROYA

Accession

Primary (citable) accession number: B4PL32

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 23, 2008
Last modified:	November 16, 2011
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project

Relevant documents

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents