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B4PF83

- LIAS2_DROYA

UniProt

B4PF83 - LIAS2_DROYA

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Protein

Lipoyl synthase 2, mitochondrial

Gene

Las2

Organism
Drosophila yakuba (Fruit fly)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi106 – 1061Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi111 – 1111Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi117 – 1171Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi137 – 1371Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi141 – 1411Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi144 – 1441Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase 2, mitochondrial (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lipoate synthase 2UniRule annotation
Short name:
LS 2UniRule annotation
Short name:
Lip-syn 2UniRule annotation
Lipoic acid synthase 2UniRule annotation
Gene namesi
Name:Las2UniRule annotation
ORF Names:GE22400
OrganismiDrosophila yakuba (Fruit fly)
Taxonomic identifieri7245 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000002282: Chromosome 3L, UP000002282: Unassembled WGS sequence

Organism-specific databases

FlyBaseiFBgn0239623. Dyak\GE22400.

Subcellular locationi

Mitochondrion UniRule annotation

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 379Lipoyl synthase 2, mitochondrialPRO_0000398225
Transit peptidei1 – ?MitochondrionUniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi7245.FBpp0267410.

Structurei

3D structure databases

ProteinModelPortaliB4PF83.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0320.
KOiK03644.
OrthoDBiEOG7P2XS7.
PhylomeDBiB4PF83.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B4PF83 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFGMLRALKT HVEVPIVVAT RAASTNAEKL EEIRERLAKG PNFQDFVQNP
60 70 80 90 100
DNSRSEWENY EGKLRREKGE EQRLRLPPWL KTTIPVGKNY AKIKAQMREL
110 120 130 140 150
KLSTVCEEAR CPNIGECWGG GEHGTQTATI MLMGDTCTRG CRFCSVKTAR
160 170 180 190 200
KPPPLDVNEP VNTATAIASW GLDYIVLTSV DRDDLPDGGS KHIAETVREI
210 220 230 240 250
KARNSNIFVE CLVPDFRGNL ECVETIANSG LDVYAHNIET VEKLTPYVRD
260 270 280 290 300
RRAHYRQTLQ VLTEAKRFNP NLITKSSIML GLGETDEEIE STLKDLRTAG
310 320 330 340 350
VDCVTLGQYM QPTNKHLKVI EYVTPEKFKH WEERGNELGF LYTASGPLVR
360 370
SSYKAGEFFI TSILENRKKR QNATELSKE
Length:379
Mass (Da):42,853
Last modified:September 23, 2008 - v1
Checksum:i4F9A59334D54132B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CM000159 Genomic DNA. Translation: EDW95169.1.
RefSeqiXP_002095457.1. XM_002095421.1.

Genome annotation databases

EnsemblMetazoaiFBtr0268918; FBpp0267410; FBgn0239623.
GeneIDi6534785.
KEGGidya:Dyak_GE22400.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CM000159 Genomic DNA. Translation: EDW95169.1 .
RefSeqi XP_002095457.1. XM_002095421.1.

3D structure databases

ProteinModelPortali B4PF83.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 7245.FBpp0267410.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0268918 ; FBpp0267410 ; FBgn0239623 .
GeneIDi 6534785.
KEGGi dya:Dyak_GE22400.

Organism-specific databases

FlyBasei FBgn0239623. Dyak\GE22400.

Phylogenomic databases

eggNOGi COG0320.
KOi K03644.
OrthoDBi EOG7P2XS7.
PhylomeDBi B4PF83.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Evolution of genes and genomes on the Drosophila phylogeny."
    Drosophila 12 genomes consortium
    Nature 450:203-218(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Tai18E2 / Tucson 14021-0261.01.

Entry informationi

Entry nameiLIAS2_DROYA
AccessioniPrimary (citable) accession number: B4PF83
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: September 23, 2008
Last modified: October 29, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3