B4P6P6 (CALYP_DROYA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase calypso
EC=3.4.19.12

Alternative name(s):
BAP1 homolog

Gene names

Name:	calypso
ORF Names:	GE14091

Organism

Drosophila yakuba (Fruit fly) [Complete proteome]

Taxonomic identifier

7245 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	471 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Polycomb group (PcG) protein. Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-118' (H2AK118ub1). Does not deubiquitinate monoubiquitinated histone H2B. Required to maintain the transcriptionally repressive state of homeotic genes throughout development. The PR-DUB complex has weak or no activity toward 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Subunit structure	Component of the PR-DUB complex, at least composed of calypso and Asx By similarity.
Subcellular location	Nucleus By similarity. Note: Localizes to PcG response elements (PREs) By similarity.
Sequence similarities	Belongs to the peptidase C12 family. BAP1 subfamily.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Nucleus
Molecular function	Chromatin regulator Hydrolase Protease Thiol protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	chromatin silencing Inferred from sequence or structural similarity. Source: UniProtKB monoubiquitinated histone H2A deubiquitination Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	PR-DUB complex Inferred from sequence or structural similarity. Source: UniProtKB nuclear chromatin Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	chromatin binding Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin thiolesterase activity Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin-specific protease activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 471	471	Ubiquitin carboxyl-terminal hydrolase calypso		PRO_0000395835
Sites
	Active site	131	1	Nucleophile By similarity
	Active site	213	1	Proton donor By similarity
	Site	228	1	Important for enzyme activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	B4P6P6 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 2E576237C4F8ABCF	FASTA	471	51,482
	10 20 30 40 50 60 MNAAGGGSGS QAAGAAGVNS SLSHNALLST ASGATTMPMA QLADGWLELE SDPGLFTLLL 70 80 90 100 110 120 KDFGCHDVQV EEVYDLQKPI ESPYGFIFLF RWIEERRARR KIVETTAEIF VKDEEAISSI 130 140 150 160 170 180 FFAQQVVPNS CATHALLSVL LNCNENNLQL GDTLSRLKAH TKGMSPENKG LAIGNTPELA 190 200 210 220 230 240 CAHNSHAMPQ ARRRLERTGA GVSSCRFTGE AFHFVSFVPI NGQLFELDGL KPYPMNHGGW 250 260 270 280 290 300 EDSEDWTDKF RRVMAERLGI ATGEQDIRFN LMAVVPDRRI AITHKLKMLR TNQAIVSGTL 310 320 330 340 350 360 QKLLKADEQG ESGNGDSQRP DTPTTLLEPS AFTARDLQSL LKNLDTEIAI NEQHLADEND 370 380 390 400 410 420 RRHMFKVDAS RRTHNYDKFI CTFLSMLAHQ GVLGELVSQH LLPSKKVSGQ GAANRISKQS 430 440 450 460 470 TTASAGGSTT GATASTPKTQ QQQAAAAKNG KSPSKTPGRR RKGRNKCRKR K

References

[1]	"Evolution of genes and genomes on the Drosophila phylogeny." Drosophila 12 genomes consortium Nature 450:203-218(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tai18E2 / Tucson 14021-0261.01.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CM000158 Genomic DNA. Translation: EDW91973.1.
RefSeq	XP_002092261.1. XM_002092225.1.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	C12.A09.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0260609; FBpp0259101; FBgn0231717.
GeneID	6531462.
KEGG	dya:Dyak_GE14091.
Organism-specific databases
FlyBase	FBgn0231717. Dyak\GE14091.
Phylogenomic databases
eggNOG	NOG249036.
KO	K08588.
OrthoDB	EOG4FN302.
Family and domain databases
Gene3D	3.40.532.10. 1 hit.
InterPro	IPR001578. Peptidase_C12. [Graphical view]
PANTHER	PTHR10589. PTHR10589. 1 hit.
Pfam	PF01088. Peptidase_C12. 1 hit. [Graphical view]
PRINTS	PR00707. UBCTHYDRLASE.
PROSITE	PS00140. UCH_1. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CALYP_DROYA

Accession

Primary (citable) accession number: B4P6P6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 13, 2010
Last sequence update:	September 23, 2008
Last modified:	March 6, 2013
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project