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B4NBP4 (SPAST_DROWI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 25. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Spastin
EC=3.6.4.3
Gene names
Name:spas
ORF Names:GK11148
OrganismDrosophila willistoni (Fruit fly) [Complete proteome]
Taxonomic identifier7260 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length777 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

ATP-dependent microtubule severing protein. Stimulates microtubule minus-end depolymerization and poleward microtubule flux in the mitotic spindle. Regulates microtubule stability in the neuromuscular junction synapse By similarity.

Catalytic activity

ATP + H2O = ADP + phosphate.

Subunit structure

Homohexamer. The homohexamer is stabilized by ATP-binding. The homohexamer may adopt a ring conformation through which microtubules pass prior to being severed. Interacts with microtubules By similarity.

Subcellular location

Membrane; Single-pass membrane protein Potential. Cytoplasm › cytoskeleton › centrosome By similarity. Cytoplasm › cytoskeleton By similarity. Chromosome By similarity. Note: Colocalizes with cellular microtubule arrays. Localizes to chromosomes from prometaphase/metaphase to anaphase, and this requires microtubules. Localizes to discrete punctate cytoplasmic foci which may correspond to secretory vesicles By similarity.

Sequence similarities

Belongs to the AAA ATPase family. Spastin subfamily.

Contains 1 MIT domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 777777Spastin
PRO_0000367151

Regions

Transmembrane110 – 12920Helical; Potential
Domain237 – 31377MIT
Nucleotide binding542 – 5498ATP Potential
Region1 – 215215Required for localization to punctate cytoplasmic foci By similarity
Region213 – 777565Sufficient for interaction with microtubules and microtubule severing By similarity
Region462 – 47413Required for interaction with microtubules By similarity

Amino acid modifications

Modified residue4581Phosphothreonine By similarity

Sequences

Sequence LengthMass (Da)Tools
B4NBP4 [UniParc].

Last modified September 23, 2008. Version 1.
Checksum: 9C3C6569E4BAD42D

FASTA77785,093
        10         20         30         40         50         60 
MVRTKSSSSS ASSSSQKSPI KSNNGAGGGG SSSSHRQSHR TSIDERKSSS HAHSNNSNVS 

        70         80         90        100        110        120 
SSSRRAATAT SGSSSPEGDD DTTTDDLTPT GSSPRSCNGR GHSSVHKQNL YVVSFPIIFL 

       130        140        150        160        170        180 
FNVLRSLIYQ LFCIFRYLYG ASTKVLYRPH RRDCNIEIVV QNSKEQQLQL QQHQHNQTLS 

       190        200        210        220        230        240 
YSLETGGVSG GSGGEQQVQV QPQRIRALQP LEMATNRPGG GYSPGPGDPL LAKQKHHHRR 

       250        260        270        280        290        300 
AFEYISKALK IDEENEGHKE LAIELYRKGI KELEDGIAVD CWNGRGDVWD RAQRLHDKMQ 

       310        320        330        340        350        360 
TNLSMARDRL HFLALREEDL QMQRLSLKEK QPAPKQPQRS QTKDPVKQPM LTSLNADPVK 

       370        380        390        400        410        420 
MKVRSSGYGP KQNGTSSSRP APSGQTATGA SGRKLTVGTK RPGNLPVTNK SQTLPRNLGS 

       430        440        450        460        470        480 
KTTVGAVQRQ PAKTAATPPA VRRQFSSGRN TPPQRSRTPI NNNASSGSGA STPMVSVKGV 

       490        500        510        520        530        540 
EQKLVQLILD EIVEGGAKVE WTDIAGQDVA KQALQEMVIL PSVRPELFTG LRAPAKGLLL 

       550        560        570        580        590        600 
FGPPGNGKTL LARAVATECS ATFLNISAAS LTSKYVGDGE KLVRALFAVA RHMQPSIIFI 

       610        620        630        640        650        660 
DEVDSLLSER SSNEHEASRR LKTEFLVEFD GLPGNPDGDR IVVLAATNRP QELDEAALRR 

       670        680        690        700        710        720 
FTKRVYVSLP DEQTRELLLN RLLQKQGSPL DTEALRRLAK ITEGYSGSDL TALAKDAALE 

       730        740        750        760        770 
PIRELNVEQV KCLDISAMRP ITEKDFHNSL KRIRRSVAPQ SLNSYEKWSQ DYGDITI

« Hide

References

[1]"Evolution of genes and genomes on the Drosophila phylogeny."
Drosophila 12 genomes consortium
Nature 450:203-218(2007) [PubMed: 17994087] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Tucson 14030-0811.24.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CH964232 Genomic DNA. Translation: EDW81208.1.
RefSeqXP_002070222.1. XM_002070186.1.

3D structure databases

ProteinModelPortalB4NBP4.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0241799; FBpp0240291; FBgn0213159.
GeneID6648037.
KEGGdwi:Dwil_GK11148.

Organism-specific databases

FlyBaseFBgn0213159. Dwil\GK11148.

Phylogenomic databases

GeneTreeEMGT00050000004565.
InParanoidB4NBP4.
OMAFLNISAA.
OrthoDBEOG44XGXW.
PhylomeDBB4NBP4.

Family and domain databases

InterProIPR003593. ATPase_AAA+_core.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR007330. MIT.
[Graphical view]
KOK13254.
PfamPF00004. AAA. 1 hit.
PF04212. MIT. 1 hit.
[Graphical view]
SMARTSM00382. AAA. 1 hit.
SM00745. MIT. 1 hit.
[Graphical view]
PROSITEPS00674. AAA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSPAST_DROWI
AccessionPrimary (citable) accession number: B4NBP4
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 23, 2008
Last modified: January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase

SIMILARITY comments

Index of protein domains and families

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