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B4NAL6 (PDE6_DROWI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
cGMP-specific 3',5'-cyclic phosphodiesterase

EC=3.1.4.35
Gene names
Name:Pde6
ORF Names:GK11355
OrganismDrosophila willistoni (Fruit fly) [Complete proteome]
Taxonomic identifier7260 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length1127 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Has a role regulating cGMP transport in Malpighian tubule principal cells By similarity. UniProtKB Q9VFI9

Catalytic activity

Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate. UniProtKB Q9VFI9

Cofactor

Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.

Subunit structure

Interacts with PrBP By similarity. UniProtKB Q9VFI9

Subcellular location

Cell membrane; Lipid-anchor; Cytoplasmic side By similarity UniProtKB Q9VFI9.

Sequence similarities

Belongs to the cyclic nucleotide phosphodiesterase family.

Contains 2 GAF domains.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11241124cGMP-specific 3',5'-cyclic phosphodiesterase UniProtKB Q9VFI9
PRO_0000363702
Propeptide1125 – 11273Removed in mature form By similarity UniProtKB Q9VFI9
PRO_0000363703

Regions

Domain255 – 407153GAF 1
Domain439 – 624186GAF 2

Sites

Active site7301Proton donor By similarity
Metal binding7341Divalent metal cation 1 By similarity
Metal binding7701Divalent metal cation 1 By similarity
Metal binding7711Divalent metal cation 1 By similarity
Metal binding7711Divalent metal cation 2 By similarity
Metal binding8811Divalent metal cation 1 By similarity

Amino acid modifications

Modified residue11241Cysteine methyl ester By similarity UniProtKB Q9VFI9
Lipidation11241S-farnesyl cysteine By similarity UniProtKB Q9VFI9

Sequences

Sequence LengthMass (Da)Tools
B4NAL6 [UniParc].

Last modified September 23, 2008. Version 1.
Checksum: 32473E517D9B55C5

FASTA1,127125,223
        10         20         30         40         50         60 
MTDVSAAAGG ATAEPPTATM SPTSVEPLTN GAKKSAMATT TETQTAAAAA AATTTPAATT 

        70         80         90        100        110        120 
TVTVTAAATT EASTSNQVKL ARVENVLGST STSSPANETK PKSLPDGGNA SVAPASSSSN 

       130        140        150        160        170        180 
SISTQTLIQS QSQPHAKTNK STTVASQQDV DEVARLFEEK PEAFEKWLTE RAPPEALSRL 

       190        200        210        220        230        240 
HEFIESRKPP KRPSVTSDLF QQWMASSPTV QQKSPGRSLS NSSASSLPEC RRHLMDLDEG 

       250        260        270        280        290        300 
ELFMELIRDV ANELDIDVLC HKILVNVGLL THADRGSLFL AKGTANNKYL VAKLFDVTQK 

       310        320        330        340        350        360 
TALKDAVTRA STEEIIIPFG IGIAGMVAQT KQMINIKEAY KDARFNCEID LKTGYKTNAI 

       370        380        390        400        410        420 
LCMPICNYEG DIIGVAQIIN KTNGCMEFDE HDVEIFRRYL TFCGIGIQNA QLFEMSVQEY 

       430        440        450        460        470        480 
RRNQILLNLA RSIFEEQNNL ECLVTKIMTE ARELLKCERC SVFLVDLDCC EASHLEKIIE 

       490        500        510        520        530        540 
KPNQLEQRPS RAIKSADSFE EKKLRNRFTV LFELGGEYQA ANVSRPSISE LSHSTLAQIA 

       550        560        570        580        590        600 
QFVATTGQTV NICDVNEWVR EHNHQIRTES EIDSTQAILC MPIVNAQKTV IGVAQLINKA 

       610        620        630        640        650        660 
NGVPFTESDA SIFEAFAIFC GLGIHNTQMY ENACKLMAKQ KVALECLSYH ATANQDQTEK 

       670        680        690        700        710        720 
LTQDVVAEAE SYNLYSFTFT DFDLVDDDTC RAVLRMFMQC NLVSQFQIPY DVLCRWVLSV 

       730        740        750        760        770        780 
RKNYRPVKYH NWRHALNVAQ TMFAMLKTGK MERFMTDLEI LGLLVACLCH DLDHRGTNNA 

       790        800        810        820        830        840 
FQTKTESPLA ILYTTSTMEH HHFDQCVMIL NSEGNNIFQA LSPEDYRSVM KTVESAILST 

       850        860        870        880        890        900 
DLAMYFKKRN AFLELVENGE FDWQGEEKKD LLCGMMMTAC DVSAIAKPWE VQHRVAKLVA 

       910        920        930        940        950        960 
DEFFDQGDLE KLQLNTQPVA MMDRERKDEL PKMQVGFIDV ICLPLYRVLC DTFPWITPLY 

       970        980        990       1000       1010       1020 
EGTLENRRNW QDLAEKVEMG LTWIDHDTID KPVEEFAGCA DEDIKDIEFT VQTLNCNQQS 

      1030       1040       1050       1060       1070       1080 
QETDAHTTPE HHRSGSRLSM KKTGALGKAV RSKLSKTLYN SMDGSKPKTS LKLLESHVSE 

      1090       1100       1110       1120 
DMDDKSPTSP SQPHGGSVGR MSASSSTSSA GTVVDKTKKR SKLCALL 

« Hide

References

[1]"Evolution of genes and genomes on the Drosophila phylogeny."
Drosophila 12 genomes consortium
Nature 450:203-218(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Tucson 14030-0811.24.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CH964232 Genomic DNA. Translation: EDW80830.1.
RefSeqXP_002069844.1. XM_002069808.1.

3D structure databases

ProteinModelPortalB4NAL6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING7260.FBpp0240498.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0242006; FBpp0240498; FBgn0213366.
GeneID6647390.
KEGGdwi:Dwil_GK11355.

Organism-specific databases

FlyBaseFBgn0213366. Dwil\GK11355.

Phylogenomic databases

eggNOGNOG270709.
InParanoidB4NAL6.
KOK13763.
OMAQVIRINE.
OrthoDBEOG7RRF69.
PhylomeDBB4NAL6.

Family and domain databases

Gene3D1.10.1300.10. 1 hit.
3.30.450.40. 3 hits.
InterProIPR003018. GAF.
IPR029016. GAF_dom_like.
IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamPF01590. GAF. 2 hits.
PF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSPR00387. PDIESTERASE1.
SMARTSM00065. GAF. 2 hits.
SM00471. HDc. 1 hit.
[Graphical view]
SUPFAMSSF55781. SSF55781. 3 hits.
PROSITEPS00126. PDEASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePDE6_DROWI
AccessionPrimary (citable) accession number: B4NAL6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: September 23, 2008
Last modified: July 9, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase