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B4N278 (TRMB_DROWI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
ORF Names:GK16167
OrganismDrosophila willistoni (Fruit fly) [Complete proteome]
Taxonomic identifier7260 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length251 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_03055

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_03055

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_03055

Subcellular location

Nucleus By similarity HAMAP-Rule MF_03055.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentNucleus
   LigandRNA-binding
S-adenosyl-L-methionine
tRNA-binding
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 251251tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_03055
PRO_0000370577

Regions

Region95 – 962S-adenosyl-L-methionine binding By similarity
Region132 – 1332S-adenosyl-L-methionine binding By similarity
Region230 – 2323S-adenosyl-L-methionine binding By similarity

Sites

Active site1551 By similarity
Binding site721S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site1521S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
B4N278 [UniParc].

Last modified September 23, 2008. Version 1.
Checksum: 08997CB61ADD14D1

FASTA25128,902
        10         20         30         40         50         60 
MVTTSQEQGH DALTATSAVT GLPQKRFYRQ RAHSNPIADH SFDYPARPED VDWSALYPNI 

        70         80         90        100        110        120 
QPDQQVEFAD IGCGYGGFLV TLGEMFPQKL SIGMEIRVKV SDYVVDRIAA LRLKSGKLNG 

       130        140        150        160        170        180 
DAYKNIACIR TNAMKYLPNY FRKGQLEKMF FLYPDPHFKR AKHKWRIINQ ALLSEYAYVL 

       190        200        210        220        230        240 
RQGGLVYTMT DVEDLHQWIV SHMNQHPLYE RISPEDESQD PITPKLYQSS EEGAKVVRNK 

       250 
GDHFLAIFRR I 

« Hide

References

[1]"Evolution of genes and genomes on the Drosophila phylogeny."
Drosophila 12 genomes consortium
Nature 450:203-218(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Tucson 14030-0811.24.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CH963925 Genomic DNA. Translation: EDW78467.1.
RefSeqXP_002067481.1. XM_002067445.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING7260.FBpp0245310.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0246818; FBpp0245310; FBgn0218169.
GeneID6644754.
KEGGdwi:Dwil_GK16167.

Organism-specific databases

FlyBaseFBgn0218169. Dwil\GK16167.

Phylogenomic databases

eggNOGCOG0220.
InParanoidB4N278.
KOK03439.
OMARAHSNPI.
OrthoDBEOG7PS1GC.

Enzyme and pathway databases

UniPathwayUPA00989.

Family and domain databases

HAMAPMF_03055. tRNA_methyltr_TrmB_euk.
InterProIPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_DROWI
AccessionPrimary (citable) accession number: B4N278
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: September 23, 2008
Last modified: April 16, 2014
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase