B4N278 (TRMB_DROWI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33

Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase

Gene names

ORF Names:

GK16167

Organism

Drosophila willistoni (Fruit fly) [Complete proteome]

Taxonomic identifier

7260 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › willistoni group › willistoni subgroup

Protein attributes

Sequence length	251 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA.
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Nucleus
Ligand	RNA-binding S-adenosyl-L-methionine tRNA-binding
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	RNA (guanine-N7)-methylation Inferred from electronic annotation. Source: GOC
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	tRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 251	251	tRNA (guanine-N(7)-)-methyltransferase		PRO_0000370577
Regions
	Region	95 – 96	2	S-adenosyl-L-methionine binding By similarity
	Region	132 – 133	2	S-adenosyl-L-methionine binding By similarity
	Region	230 – 232	3	S-adenosyl-L-methionine binding By similarity
Sites
	Active site	155	1	By similarity
	Binding site	72	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	152	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	B4N278 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 08997CB61ADD14D1	FASTA	251	28,902
	10 20 30 40 50 60 MVTTSQEQGH DALTATSAVT GLPQKRFYRQ RAHSNPIADH SFDYPARPED VDWSALYPNI 70 80 90 100 110 120 QPDQQVEFAD IGCGYGGFLV TLGEMFPQKL SIGMEIRVKV SDYVVDRIAA LRLKSGKLNG 130 140 150 160 170 180 DAYKNIACIR TNAMKYLPNY FRKGQLEKMF FLYPDPHFKR AKHKWRIINQ ALLSEYAYVL 190 200 210 220 230 240 RQGGLVYTMT DVEDLHQWIV SHMNQHPLYE RISPEDESQD PITPKLYQSS EEGAKVVRNK 250 GDHFLAIFRR I

References

[1]	"Evolution of genes and genomes on the Drosophila phylogeny." Drosophila 12 genomes consortium Nature 450:203-218(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tucson 14030-0811.24.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH963925 Genomic DNA. Translation: EDW78467.1.
RefSeq	XP_002067481.1. XM_002067445.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	7260.FBpp0245310.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0246818; FBpp0245310; FBgn0218169.
GeneID	6644754.
KEGG	dwi:Dwil_GK16167.
Organism-specific databases
FlyBase	FBgn0218169. Dwil\GK16167.
Phylogenomic databases
eggNOG	COG0220.
InParanoid	B4N278.
KO	K03439.
OMA	RAHSNPI.
OrthoDB	EOG4PC887.
Enzyme and pathway databases
UniPathway	UPA00989.
Family and domain databases
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. IPR025763. tRNA_(Gua-N-7)_MeTrfase_euk. [Graphical view]
Pfam	PF02390. Methyltransf_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00091. TIGR00091. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMB_DROWI

Accession

Primary (citable) accession number: B4N278

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	September 23, 2008
Last modified:	April 3, 2013
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project