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B4N278

- TRMB_DROWI

UniProt

B4N278 - TRMB_DROWI

Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

GK16167

Organism
Drosophila willistoni (Fruit fly)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 38 (01 Oct 2014)
      Sequence version 1 (23 Sep 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

    Catalytic activityi

    S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei72 – 721S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
    Binding sitei152 – 1521S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
    Active sitei155 – 1551UniRule annotation

    GO - Molecular functioni

    1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
    2. tRNA binding Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    RNA-binding, S-adenosyl-L-methionine, tRNA-binding

    Enzyme and pathway databases

    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
    tRNA(m7G46)-methyltransferaseUniRule annotation
    Gene namesi
    ORF Names:GK16167
    OrganismiDrosophila willistoni (Fruit fly)
    Taxonomic identifieri7260 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000007798: Unassembled WGS sequence

    Organism-specific databases

    FlyBaseiFBgn0218169. Dwil\GK16167.

    Subcellular locationi

    Nucleus UniRule annotation

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 251251tRNA (guanine-N(7)-)-methyltransferasePRO_0000370577Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi7260.FBpp0245310.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni95 – 962S-adenosyl-L-methionine bindingUniRule annotation
    Regioni132 – 1332S-adenosyl-L-methionine bindingUniRule annotation
    Regioni230 – 2323S-adenosyl-L-methionine bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0220.
    InParanoidiB4N278.
    KOiK03439.
    OMAiRAHSNPI.
    OrthoDBiEOG7PS1GC.
    PhylomeDBiB4N278.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR025763. Trm8_euk.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B4N278-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVTTSQEQGH DALTATSAVT GLPQKRFYRQ RAHSNPIADH SFDYPARPED    50
    VDWSALYPNI QPDQQVEFAD IGCGYGGFLV TLGEMFPQKL SIGMEIRVKV 100
    SDYVVDRIAA LRLKSGKLNG DAYKNIACIR TNAMKYLPNY FRKGQLEKMF 150
    FLYPDPHFKR AKHKWRIINQ ALLSEYAYVL RQGGLVYTMT DVEDLHQWIV 200
    SHMNQHPLYE RISPEDESQD PITPKLYQSS EEGAKVVRNK GDHFLAIFRR 250
    I 251
    Length:251
    Mass (Da):28,902
    Last modified:September 23, 2008 - v1
    Checksum:i08997CB61ADD14D1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH963925 Genomic DNA. Translation: EDW78467.1.
    RefSeqiXP_002067481.1. XM_002067445.1.

    Genome annotation databases

    EnsemblMetazoaiFBtr0246818; FBpp0245310; FBgn0218169.
    GeneIDi6644754.
    KEGGidwi:Dwil_GK16167.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH963925 Genomic DNA. Translation: EDW78467.1 .
    RefSeqi XP_002067481.1. XM_002067445.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 7260.FBpp0245310.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0246818 ; FBpp0245310 ; FBgn0218169 .
    GeneIDi 6644754.
    KEGGi dwi:Dwil_GK16167.

    Organism-specific databases

    FlyBasei FBgn0218169. Dwil\GK16167.

    Phylogenomic databases

    eggNOGi COG0220.
    InParanoidi B4N278.
    KOi K03439.
    OMAi RAHSNPI.
    OrthoDBi EOG7PS1GC.
    PhylomeDBi B4N278.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_03055. tRNA_methyltr_TrmB_euk.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR025763. Trm8_euk.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Evolution of genes and genomes on the Drosophila phylogeny."
      Drosophila 12 genomes consortium
      Nature 450:203-218(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Tucson 14030-0811.24.

    Entry informationi

    Entry nameiTRMB_DROWI
    AccessioniPrimary (citable) accession number: B4N278
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 5, 2009
    Last sequence update: September 23, 2008
    Last modified: October 1, 2014
    This is version 38 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3