Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

GK16167

Organism
Drosophila willistoni (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei72S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei152S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Active sitei155UniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase, tRNA-binding
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
ORF Names:GK16167
OrganismiDrosophila willistoni (Fruit fly)
Taxonomic identifieri7260 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000007798 Componenti: Unassembled WGS sequence

Organism-specific databases

FlyBaseiFBgn0218169. Dwil\GK16167.

Subcellular locationi

  • Nucleus UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003705771 – 251tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST251

Interactioni

Protein-protein interaction databases

STRINGi7260.FBpp0245310.

Structurei

3D structure databases

SMRiB4N278.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni95 – 96S-adenosyl-L-methionine bindingUniRule annotation2
Regioni132 – 133S-adenosyl-L-methionine bindingUniRule annotation2
Regioni230 – 232S-adenosyl-L-methionine bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiKOG3115. Eukaryota.
COG0220. LUCA.
InParanoidiB4N278.
KOiK03439.
OMAiKWRIINQ.
OrthoDBiEOG091G0IQ7.
PhylomeDBiB4N278.

Family and domain databases

HAMAPiMF_03055. tRNA_methyltr_TrmB_euk. 1 hit.
InterProiView protein in InterPro
IPR029063. SAM-dependent_MTases.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
PANTHERiPTHR23417. PTHR23417. 1 hit.
PTHR23417:SF20. PTHR23417:SF20. 1 hit.
PfamiView protein in Pfam
PF02390. Methyltransf_4. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiView protein in PROSITE
PS51625. SAM_MT_TRMB. 1 hit.

Sequencei

Sequence statusi: Complete.

B4N278-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTTSQEQGH DALTATSAVT GLPQKRFYRQ RAHSNPIADH SFDYPARPED
60 70 80 90 100
VDWSALYPNI QPDQQVEFAD IGCGYGGFLV TLGEMFPQKL SIGMEIRVKV
110 120 130 140 150
SDYVVDRIAA LRLKSGKLNG DAYKNIACIR TNAMKYLPNY FRKGQLEKMF
160 170 180 190 200
FLYPDPHFKR AKHKWRIINQ ALLSEYAYVL RQGGLVYTMT DVEDLHQWIV
210 220 230 240 250
SHMNQHPLYE RISPEDESQD PITPKLYQSS EEGAKVVRNK GDHFLAIFRR

I
Length:251
Mass (Da):28,902
Last modified:September 23, 2008 - v1
Checksum:i08997CB61ADD14D1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH963925 Genomic DNA. Translation: EDW78467.1.
RefSeqiXP_002067481.1. XM_002067445.2.

Genome annotation databases

EnsemblMetazoaiFBtr0246818; FBpp0245310; FBgn0218169.
GeneIDi6644754.
KEGGidwi:Dwil_GK16167.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH963925 Genomic DNA. Translation: EDW78467.1.
RefSeqiXP_002067481.1. XM_002067445.2.

3D structure databases

SMRiB4N278.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7260.FBpp0245310.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0246818; FBpp0245310; FBgn0218169.
GeneIDi6644754.
KEGGidwi:Dwil_GK16167.

Organism-specific databases

FlyBaseiFBgn0218169. Dwil\GK16167.

Phylogenomic databases

eggNOGiKOG3115. Eukaryota.
COG0220. LUCA.
InParanoidiB4N278.
KOiK03439.
OMAiKWRIINQ.
OrthoDBiEOG091G0IQ7.
PhylomeDBiB4N278.

Enzyme and pathway databases

UniPathwayiUPA00989.

Family and domain databases

HAMAPiMF_03055. tRNA_methyltr_TrmB_euk. 1 hit.
InterProiView protein in InterPro
IPR029063. SAM-dependent_MTases.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
PANTHERiPTHR23417. PTHR23417. 1 hit.
PTHR23417:SF20. PTHR23417:SF20. 1 hit.
PfamiView protein in Pfam
PF02390. Methyltransf_4. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiView protein in PROSITE
PS51625. SAM_MT_TRMB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTRMB_DROWI
AccessioniPrimary (citable) accession number: B4N278
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: September 23, 2008
Last modified: May 10, 2017
This is version 48 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.