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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

GK16167

Organism
Drosophila willistoni (Fruit fly)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei72S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei152S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Active sitei155UniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase, tRNA-binding
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
ORF Names:GK16167
OrganismiDrosophila willistoni (Fruit fly)
Taxonomic identifieri7260 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000007798 Componenti: Unassembled WGS sequence

Organism-specific databases

FlyBaseiFBgn0218169 Dwil\GK16167

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003705771 – 251tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST251

Proteomic databases

PRIDEiB4N278

Interactioni

Protein-protein interaction databases

STRINGi7260.FBpp0245310

Structurei

3D structure databases

SMRiB4N278
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni95 – 96S-adenosyl-L-methionine bindingUniRule annotation2
Regioni132 – 133S-adenosyl-L-methionine bindingUniRule annotation2
Regioni230 – 232S-adenosyl-L-methionine bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiKOG3115 Eukaryota
COG0220 LUCA
InParanoidiB4N278
KOiK03439
OMAiIGMEIRV
OrthoDBiEOG091G0IQ7
PhylomeDBiB4N278

Family and domain databases

HAMAPiMF_03055 tRNA_methyltr_TrmB_euk, 1 hit
InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR025763 Trm8_euk
IPR003358 tRNA_(Gua-N-7)_MeTrfase_Trmb
PANTHERiPTHR23417 PTHR23417, 1 hit
PTHR23417:SF16 PTHR23417:SF16, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00091 TIGR00091, 1 hit
PROSITEiView protein in PROSITE
PS51625 SAM_MT_TRMB, 1 hit

Sequencei

Sequence statusi: Complete.

B4N278-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTTSQEQGH DALTATSAVT GLPQKRFYRQ RAHSNPIADH SFDYPARPED
60 70 80 90 100
VDWSALYPNI QPDQQVEFAD IGCGYGGFLV TLGEMFPQKL SIGMEIRVKV
110 120 130 140 150
SDYVVDRIAA LRLKSGKLNG DAYKNIACIR TNAMKYLPNY FRKGQLEKMF
160 170 180 190 200
FLYPDPHFKR AKHKWRIINQ ALLSEYAYVL RQGGLVYTMT DVEDLHQWIV
210 220 230 240 250
SHMNQHPLYE RISPEDESQD PITPKLYQSS EEGAKVVRNK GDHFLAIFRR

I
Length:251
Mass (Da):28,902
Last modified:September 23, 2008 - v1
Checksum:i08997CB61ADD14D1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH963925 Genomic DNA Translation: EDW78467.1
RefSeqiXP_002067481.1, XM_002067445.2

Genome annotation databases

EnsemblMetazoaiFBtr0246818; FBpp0245310; FBgn0218169
GeneIDi6644754
KEGGidwi:Dwil_GK16167

Similar proteinsi

Entry informationi

Entry nameiTRMB_DROWI
AccessioniPrimary (citable) accession number: B4N278
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: September 23, 2008
Last modified: May 23, 2018
This is version 52 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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