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B4MXR6

- LIAS_DROWI

UniProt

B4MXR6 - LIAS_DROWI

Protein

Lipoyl synthase, mitochondrial

Gene

Las

Organism
Drosophila willistoni (Fruit fly)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 40 (01 Oct 2014)
      Sequence version 1 (23 Sep 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi103 – 1031Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi108 – 1081Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi114 – 1141Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi134 – 1341Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi138 – 1381Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi141 – 1411Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lipoate synthaseUniRule annotation
    Short name:
    LSUniRule annotation
    Short name:
    Lip-synUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Gene namesi
    Name:LasUniRule annotation
    ORF Names:GK17689
    OrganismiDrosophila willistoni (Fruit fly)
    Taxonomic identifieri7260 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000007798: Unassembled WGS sequence

    Organism-specific databases

    FlyBaseiFBgn0219688. Dwil\GK17689.

    Subcellular locationi

    Mitochondrion UniRule annotation

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 372Lipoyl synthase, mitochondrialPRO_0000398223
    Transit peptidei1 – ?MitochondrionUniRule annotation

    Interactioni

    Protein-protein interaction databases

    STRINGi7260.FBpp0246832.

    Structurei

    3D structure databases

    ProteinModelPortaliB4MXR6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0320.
    InParanoidiB4MXR6.
    KOiK03644.
    OMAiHPHIPTK.
    OrthoDBiEOG7P2XS7.
    PhylomeDBiB4MXR6.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    B4MXR6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLRTIKSPIK VIVRPASASA SANAEKLDEI RERLAKGPSF QDFIQNPNNT    50
    KNEWDNYDGK LRREKVEEQR LRLPPWLKTT IPMGKNYAKI KDQLRELKLS 100
    TVCEEARCPN IGECWGGGEH GTQTATIMLM GDTCTRGCRF CSVKTARAPP 150
    PLDENEPVNT AKAVASWGLD YIVLTSVDRD DLSDGGSKHI AQTVKEIKAR 200
    NQNIFVECLV PDFRGDLECV KTIANCGLDV YAHNIETVEK LTPFVRDRRA 250
    HYRQTLKVLS EAKRFNPNLI TKSSIMLGLG ETDAEVEQTM LDLREVGVEC 300
    LTLGQYMQPT KKHLKVIEYV TPEKFKHWEE RGNQLGFLYT ASGPLVRSSY 350
    KAGEFFITSI LANRKKQNDA PK 372
    Length:372
    Mass (Da):41,989
    Last modified:September 23, 2008 - v1
    Checksum:i49A97DBE8AD6E2A8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH963876 Genomic DNA. Translation: EDW76835.1.
    RefSeqiXP_002065849.1. XM_002065813.1.

    Genome annotation databases

    EnsemblMetazoaiFBtr0248340; FBpp0246832; FBgn0219688.
    GeneIDi6642928.
    KEGGidwi:Dwil_GK17689.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH963876 Genomic DNA. Translation: EDW76835.1 .
    RefSeqi XP_002065849.1. XM_002065813.1.

    3D structure databases

    ProteinModelPortali B4MXR6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 7260.FBpp0246832.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0248340 ; FBpp0246832 ; FBgn0219688 .
    GeneIDi 6642928.
    KEGGi dwi:Dwil_GK17689.

    Organism-specific databases

    FlyBasei FBgn0219688. Dwil\GK17689.

    Phylogenomic databases

    eggNOGi COG0320.
    InParanoidi B4MXR6.
    KOi K03644.
    OMAi HPHIPTK.
    OrthoDBi EOG7P2XS7.
    PhylomeDBi B4MXR6.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Evolution of genes and genomes on the Drosophila phylogeny."
      Drosophila 12 genomes consortium
      Nature 450:203-218(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Tucson 14030-0811.24.

    Entry informationi

    Entry nameiLIAS_DROWI
    AccessioniPrimary (citable) accession number: B4MXR6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: September 23, 2008
    Last modified: October 1, 2014
    This is version 40 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3