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B4MXR6

- LIAS_DROWI

UniProt

B4MXR6 - LIAS_DROWI

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Protein

Lipoyl synthase, mitochondrial

Gene

Las

Organism
Drosophila willistoni (Fruit fly)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi103 – 1031Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi108 – 1081Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi114 – 1141Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi134 – 1341Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi138 – 1381Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi141 – 1411Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lipoate synthaseUniRule annotation
Short name:
LSUniRule annotation
Short name:
Lip-synUniRule annotation
Lipoic acid synthaseUniRule annotation
Gene namesi
Name:LasUniRule annotation
ORF Names:GK17689
OrganismiDrosophila willistoni (Fruit fly)
Taxonomic identifieri7260 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000007798: Unassembled WGS sequence

Organism-specific databases

FlyBaseiFBgn0219688. Dwil\GK17689.

Subcellular locationi

Mitochondrion UniRule annotation

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 372Lipoyl synthase, mitochondrialPRO_0000398223
Transit peptidei1 – ?MitochondrionUniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi7260.FBpp0246832.

Structurei

3D structure databases

ProteinModelPortaliB4MXR6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0320.
InParanoidiB4MXR6.
KOiK03644.
OMAiHPHIPTK.
OrthoDBiEOG7P2XS7.
PhylomeDBiB4MXR6.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B4MXR6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLRTIKSPIK VIVRPASASA SANAEKLDEI RERLAKGPSF QDFIQNPNNT
60 70 80 90 100
KNEWDNYDGK LRREKVEEQR LRLPPWLKTT IPMGKNYAKI KDQLRELKLS
110 120 130 140 150
TVCEEARCPN IGECWGGGEH GTQTATIMLM GDTCTRGCRF CSVKTARAPP
160 170 180 190 200
PLDENEPVNT AKAVASWGLD YIVLTSVDRD DLSDGGSKHI AQTVKEIKAR
210 220 230 240 250
NQNIFVECLV PDFRGDLECV KTIANCGLDV YAHNIETVEK LTPFVRDRRA
260 270 280 290 300
HYRQTLKVLS EAKRFNPNLI TKSSIMLGLG ETDAEVEQTM LDLREVGVEC
310 320 330 340 350
LTLGQYMQPT KKHLKVIEYV TPEKFKHWEE RGNQLGFLYT ASGPLVRSSY
360 370
KAGEFFITSI LANRKKQNDA PK
Length:372
Mass (Da):41,989
Last modified:September 23, 2008 - v1
Checksum:i49A97DBE8AD6E2A8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CH963876 Genomic DNA. Translation: EDW76835.1.
RefSeqiXP_002065849.1. XM_002065813.1.

Genome annotation databases

EnsemblMetazoaiFBtr0248340; FBpp0246832; FBgn0219688.
GeneIDi6642928.
KEGGidwi:Dwil_GK17689.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CH963876 Genomic DNA. Translation: EDW76835.1 .
RefSeqi XP_002065849.1. XM_002065813.1.

3D structure databases

ProteinModelPortali B4MXR6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 7260.FBpp0246832.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0248340 ; FBpp0246832 ; FBgn0219688 .
GeneIDi 6642928.
KEGGi dwi:Dwil_GK17689.

Organism-specific databases

FlyBasei FBgn0219688. Dwil\GK17689.

Phylogenomic databases

eggNOGi COG0320.
InParanoidi B4MXR6.
KOi K03644.
OMAi HPHIPTK.
OrthoDBi EOG7P2XS7.
PhylomeDBi B4MXR6.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Evolution of genes and genomes on the Drosophila phylogeny."
    Drosophila 12 genomes consortium
    Nature 450:203-218(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Tucson 14030-0811.24.

Entry informationi

Entry nameiLIAS_DROWI
AccessioniPrimary (citable) accession number: B4MXR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: September 23, 2008
Last modified: October 29, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3