SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

B4MXR6

- LIAS_DROWI

UniProt

B4MXR6 - LIAS_DROWI

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Lipoyl synthase, mitochondrial
Gene
Las, GK17689
Organism
Drosophila willistoni (Fruit fly)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi103 – 1031Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi108 – 1081Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi114 – 1141Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi134 – 1341Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi138 – 1381Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi141 – 1411Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrial (EC:2.8.1.8)
Alternative name(s):
Lipoate synthase
Short name:
LS
Short name:
Lip-syn
Lipoic acid synthase
Gene namesi
Name:Las
ORF Names:GK17689
OrganismiDrosophila willistoni (Fruit fly)
Taxonomic identifieri7260 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000007798: Unassembled WGS sequence

Organism-specific databases

FlyBaseiFBgn0219688. Dwil\GK17689.

Subcellular locationi

Mitochondrion By similarity UniRule annotation

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 372Lipoyl synthase, mitochondrialUniRule annotationPRO_0000398223
Transit peptidei1 – ?Mitochondrion Reviewed prediction

Interactioni

Protein-protein interaction databases

STRINGi7260.FBpp0246832.

Structurei

3D structure databases

ProteinModelPortaliB4MXR6.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0320.
InParanoidiB4MXR6.
KOiK03644.
OMAiHPHIPTK.
OrthoDBiEOG7P2XS7.
PhylomeDBiB4MXR6.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B4MXR6-1 [UniParc]FASTAAdd to Basket

« Hide

MLRTIKSPIK VIVRPASASA SANAEKLDEI RERLAKGPSF QDFIQNPNNT    50
KNEWDNYDGK LRREKVEEQR LRLPPWLKTT IPMGKNYAKI KDQLRELKLS 100
TVCEEARCPN IGECWGGGEH GTQTATIMLM GDTCTRGCRF CSVKTARAPP 150
PLDENEPVNT AKAVASWGLD YIVLTSVDRD DLSDGGSKHI AQTVKEIKAR 200
NQNIFVECLV PDFRGDLECV KTIANCGLDV YAHNIETVEK LTPFVRDRRA 250
HYRQTLKVLS EAKRFNPNLI TKSSIMLGLG ETDAEVEQTM LDLREVGVEC 300
LTLGQYMQPT KKHLKVIEYV TPEKFKHWEE RGNQLGFLYT ASGPLVRSSY 350
KAGEFFITSI LANRKKQNDA PK 372
Length:372
Mass (Da):41,989
Last modified:September 23, 2008 - v1
Checksum:i49A97DBE8AD6E2A8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CH963876 Genomic DNA. Translation: EDW76835.1.
RefSeqiXP_002065849.1. XM_002065813.1.

Genome annotation databases

EnsemblMetazoaiFBtr0248340; FBpp0246832; FBgn0219688.
GeneIDi6642928.
KEGGidwi:Dwil_GK17689.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CH963876 Genomic DNA. Translation: EDW76835.1 .
RefSeqi XP_002065849.1. XM_002065813.1.

3D structure databases

ProteinModelPortali B4MXR6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 7260.FBpp0246832.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0248340 ; FBpp0246832 ; FBgn0219688 .
GeneIDi 6642928.
KEGGi dwi:Dwil_GK17689.

Organism-specific databases

FlyBasei FBgn0219688. Dwil\GK17689.

Phylogenomic databases

eggNOGi COG0320.
InParanoidi B4MXR6.
KOi K03644.
OMAi HPHIPTK.
OrthoDBi EOG7P2XS7.
PhylomeDBi B4MXR6.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Evolution of genes and genomes on the Drosophila phylogeny."
    Drosophila 12 genomes consortium
    Nature 450:203-218(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Tucson 14030-0811.24.

Entry informationi

Entry nameiLIAS_DROWI
AccessioniPrimary (citable) accession number: B4MXR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: September 23, 2008
Last modified: July 9, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi