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Protein

Lipoyl synthase, mitochondrial

Gene

Las

Organism
Drosophila willistoni (Fruit fly)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Miscellaneous

This protein may be expected to contain an N-terminal transit peptide but none has been predicted.UniRule annotation

Catalytic activityi

Protein N6-(octanoyl)lysine + an [Fe-S] cluster scaffold protein carrying a [4Fe-4S]2+ cluster + 2 S-adenosyl-L-methionine + 2 oxidized [2Fe-2S] ferredoxin + 6 H+ = protein N6-(dihydrolipoyl)lysine + an [Fe-S] cluster scaffold protein + 2 sulfide + 4 Fe3+ + 2 L-methionine + 2 5'-deoxyadenosine + 2 reduced [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Putative lipoyltransferase 2, mitochondrial (Dwil\GK13752)
  2. Lipoyl synthase, mitochondrial (Las)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi103Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi108Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi114Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi134Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi138Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi141Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionTransferase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lipoate synthaseUniRule annotation
Short name:
LSUniRule annotation
Short name:
Lip-synUniRule annotation
Lipoic acid synthaseUniRule annotation
Gene namesi
Name:LasUniRule annotation
ORF Names:GK17689
OrganismiDrosophila willistoni (Fruit fly)
Taxonomic identifieri7260 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000007798 Componenti: Unassembled WGS sequence

Organism-specific databases

FlyBaseiFBgn0219688 Dwil\GK17689

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003982231 – 372Lipoyl synthase, mitochondrialAdd BLAST372

Proteomic databases

PRIDEiB4MXR6

Interactioni

Protein-protein interaction databases

STRINGi7260.FBpp0246832

Structurei

3D structure databases

ProteinModelPortaliB4MXR6
SMRiB4MXR6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Phylogenomic databases

eggNOGiKOG2672 Eukaryota
COG0320 LUCA
InParanoidiB4MXR6
KOiK03644
OMAiPYCDIDF
OrthoDBiEOG091G0AXJ
PhylomeDBiB4MXR6

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00206 Lipoyl_synth, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006638 Elp3/MiaB/NifB
IPR031691 LIAS_N
IPR003698 Lipoyl_synth
IPR007197 rSAM
PfamiView protein in Pfam
PF16881 LIAS_N, 1 hit
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF005963 Lipoyl_synth, 1 hit
SFLDiSFLDG01058 lipoyl_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00510 lipA, 1 hit

Sequencei

Sequence statusi: Complete.

B4MXR6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRTIKSPIK VIVRPASASA SANAEKLDEI RERLAKGPSF QDFIQNPNNT
60 70 80 90 100
KNEWDNYDGK LRREKVEEQR LRLPPWLKTT IPMGKNYAKI KDQLRELKLS
110 120 130 140 150
TVCEEARCPN IGECWGGGEH GTQTATIMLM GDTCTRGCRF CSVKTARAPP
160 170 180 190 200
PLDENEPVNT AKAVASWGLD YIVLTSVDRD DLSDGGSKHI AQTVKEIKAR
210 220 230 240 250
NQNIFVECLV PDFRGDLECV KTIANCGLDV YAHNIETVEK LTPFVRDRRA
260 270 280 290 300
HYRQTLKVLS EAKRFNPNLI TKSSIMLGLG ETDAEVEQTM LDLREVGVEC
310 320 330 340 350
LTLGQYMQPT KKHLKVIEYV TPEKFKHWEE RGNQLGFLYT ASGPLVRSSY
360 370
KAGEFFITSI LANRKKQNDA PK
Length:372
Mass (Da):41,989
Last modified:September 23, 2008 - v1
Checksum:i49A97DBE8AD6E2A8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH963876 Genomic DNA Translation: EDW76835.1
RefSeqiXP_002065849.1, XM_002065813.2

Genome annotation databases

EnsemblMetazoaiFBtr0248340; FBpp0246832; FBgn0219688
GeneIDi6642928
KEGGidwi:Dwil_GK17689

Similar proteinsi

Entry informationi

Entry nameiLIAS_DROWI
AccessioniPrimary (citable) accession number: B4MXR6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: September 23, 2008
Last modified: May 23, 2018
This is version 56 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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