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B4MLR8 (UBP36_DROWI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 32. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase 36

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme 36
Protein scrawny
Ubiquitin thioesterase 36
Ubiquitin-specific-processing protease 36
Gene names
Name:Usp36
Synonyms:scny
ORF Names:GK17299
OrganismDrosophila willistoni (Fruit fly) [Complete proteome]
Taxonomic identifier7260 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length1311 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Required for maintaining multiple types of adult stem cells, including male and female germline, epithelial follicle cell and intestinal stem cells. May function as a transcriptional repressor by continually deubiquiting histone H2B at the promoters of genes critical for cellular differentiation, thereby preventing histone H3 'Lys-4' trimethylation (H3K4) By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subunit structure

Interacts with atms/PAF1, but not with CycT By similarity.

Subcellular location

Nucleusnucleolus By similarity.

Sequence similarities

Belongs to the peptidase C19 family.

Contains 1 USP domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13111311Ubiquitin carboxyl-terminal hydrolase 36
PRO_0000378504

Regions

Domain212 – 533322USP

Sites

Active site2211Nucleophile By similarity
Active site4921Proton acceptor By similarity

Amino acid modifications

Modified residue5811Phosphoserine By similarity
Modified residue7671Phosphothreonine By similarity
Modified residue7871Phosphoserine By similarity
Modified residue7891Phosphoserine By similarity
Modified residue9821Phosphoserine By similarity
Modified residue9851Phosphothreonine By similarity
Modified residue9881Phosphoserine By similarity
Modified residue10471Phosphoserine By similarity
Modified residue10501Phosphothreonine By similarity

Sequences

Sequence LengthMass (Da)Tools
B4MLR8 [UniParc].

Last modified September 23, 2008. Version 1.
Checksum: 408909EBD862A2ED

FASTA1,311141,002
        10         20         30         40         50         60 
MPVSLAVCET TTTTTANVVN AALRESLLGG SRSGAAVAGS GSASSLISGA GGAGASAAND 

        70         80         90        100        110        120 
DDSSSTATAN TNLQNQIVAS AKRVLLAKIE YEEVENYNDS VLDNLKTKYV VIKSPTPAAA 

       130        140        150        160        170        180 
AAATNNGNSN SAGSKILGAN GHDNRQKQQN ANGGCSTPTT TTTQTSSSST SSSSIEHNNN 

       190        200        210        220        230        240 
PNELPKPKRV LYQRENIRIG WKQSERKWQI GAGMINVGNT CYLNSTLQAL FHIPALANWL 

       250        260        270        280        290        300 
NSEQSHLENC NIGGESGGGG GGGGGNNGGF CIICAMAKTL QSTQSNASAI RPYHIYSKLK 

       310        320        330        340        350        360 
QICKHMVIGR QEDAHEFLRF LVEAMEKAYL MRYRNYKELD QLVKETTPLN QIFGGYLRSE 

       370        380        390        400        410        420 
VRCLSCNHVS ITFQHFQDLL LDIRKSDTLD EAFDGYFSRE RLEDMGYKCE GCKKKVSATK 

       430        440        450        460        470        480 
QFSLQRAPIT LCIQLKRFSM IGNKLTKQIS FKPRIDLSRF AARSPTAQGQ LPLTYRLVSL 

       490        500        510        520        530        540 
VTHLGVSQHC GHYTAIGLTE SGSYYNFDDS YVRPIAMQSV CSTNSYIMFY ELDLNQPLAT 

       550        560        570        580        590        600 
PANKLNGLRQ LSNGHHHHQQ QQQQHQQQQQ QQPTVVATIA SSPMATTRFI GPQLPPGGLN 

       610        620        630        640        650        660 
GYAMTTTTTA TNNTTNGHSQ KTAIQFKPQQ NGILTVGSGK FQESGQQKSP LVGTNHKNEA 

       670        680        690        700        710        720 
PAVAPNANAN ANGKSSSNPN TNTTINTNTN NGNSNNNNTN ATTANNSNKL NQQQQQYLPM 

       730        740        750        760        770        780 
SSSDEEDSDE EKMKRPTTTT PQLPSMPKMD GGGDEKPKTL TLTPTTTPTA STPTVSNPLK 

       790        800        810        820        830        840 
RLVPYESASE EEESSSGTPS SSTPTTTTTA AAAAASSPMQ ATAAATLPPP PTPTNARKRS 

       850        860        870        880        890        900 
LPDHHHHHPH HHVMVNGHGK SPKPASMPPA TNFNSSSSKQ KTDAIDEIFK SLNNFNKKRI 

       910        920        930        940        950        960 
NNKNQKHNEG DEEEDDEETL EKETNNSSRL VSSSTNTSPT TNGWKQSQIV SSSSSNSKNV 

       970        980        990       1000       1010       1020 
STSAAAAAAT TSSSTSTSAP PSPKTPPSPA VINSKTGLWK VTRNLDDDDD EEEEDEDDEE 

      1030       1040       1050       1060       1070       1080 
HIAPTPPPVV AKTHKNPFSS QQKPTPSPST EAAPKRQKLF NGTSSSTPHV GNGYQSEPST 

      1090       1100       1110       1120       1130       1140 
PNGGGSGSGS NGCLNELLKQ SHRGYGSSSV LSWNGKQTEL DKEPFELVCA KRIAVDHGDH 

      1150       1160       1170       1180       1190       1200 
DDGGGGDDGG GVGVVTTTTT TTTTTKNTTK TLTADAQEQR QRDLDDDEEN EMDRGRQRKV 

      1210       1220       1230       1240       1250       1260 
KTATANGKSS GNSNNTTPGY NPFQEYESQK RWHNKSSNGP PRFYTQHASS SYRSNFHQRN 

      1270       1280       1290       1300       1310 
KFKCNRGGNG GGGSGGISKF DHRHGLQRHL AAGGGFPRRP NANQQQQQQQ S 

« Hide

References

[1]"Evolution of genes and genomes on the Drosophila phylogeny."
Drosophila 12 genomes consortium
Nature 450:203-218(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Tucson 14030-0811.24.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CH963847 Genomic DNA. Translation: EDW72994.1.
RefSeqXP_002062008.1. XM_002061972.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING7260.FBpp0246442.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0247950; FBpp0246442; FBgn0219298.
GeneID6638806.
KEGGdwi:Dwil_GK17299.

Organism-specific databases

FlyBaseFBgn0219298. Dwil\GK17299.

Phylogenomic databases

eggNOGCOG5533.
InParanoidB4MLR8.
KOK11855.
OMAENEMDRG.
OrthoDBEOG7XDBF6.
PhylomeDBB4MLR8.

Family and domain databases

InterProIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view]
PfamPF00443. UCH. 1 hit.
[Graphical view]
PROSITEPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUBP36_DROWI
AccessionPrimary (citable) accession number: B4MLR8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 7, 2009
Last sequence update: September 23, 2008
Last modified: July 9, 2014
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase