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B4MLR8

- UBP36_DROWI

UniProt

B4MLR8 - UBP36_DROWI

Protein

Ubiquitin carboxyl-terminal hydrolase 36

Gene

Usp36

Organism
Drosophila willistoni (Fruit fly)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 33 (01 Oct 2014)
      Sequence version 1 (23 Sep 2008)
      Previous versions | rss
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    Functioni

    Required for maintaining multiple types of adult stem cells, including male and female germline, epithelial follicle cell and intestinal stem cells. May function as a transcriptional repressor by continually deubiquiting histone H2B at the promoters of genes critical for cellular differentiation, thereby preventing histone H3 'Lys-4' trimethylation (H3K4) By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei221 – 2211NucleophilePROSITE-ProRule annotation
    Active sitei492 – 4921Proton acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW
    2. ubiquitin thiolesterase activity Source: UniProtKB

    GO - Biological processi

    1. germ-line stem cell maintenance Source: UniProtKB
    2. protein deubiquitination Source: UniProtKB
    3. somatic stem cell maintenance Source: UniProtKB
    4. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 36 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 36
    Protein scrawny
    Ubiquitin thioesterase 36
    Ubiquitin-specific-processing protease 36
    Gene namesi
    Name:Usp36
    Synonyms:scny
    ORF Names:GK17299
    OrganismiDrosophila willistoni (Fruit fly)
    Taxonomic identifieri7260 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000007798: Unassembled WGS sequence

    Organism-specific databases

    FlyBaseiFBgn0219298. Dwil\GK17299.

    Subcellular locationi

    Nucleusnucleolus By similarity

    GO - Cellular componenti

    1. nucleolus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 13111311Ubiquitin carboxyl-terminal hydrolase 36PRO_0000378504Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei581 – 5811PhosphoserineBy similarity
    Modified residuei767 – 7671PhosphothreonineBy similarity
    Modified residuei787 – 7871PhosphoserineBy similarity
    Modified residuei789 – 7891PhosphoserineBy similarity
    Modified residuei982 – 9821PhosphoserineBy similarity
    Modified residuei985 – 9851PhosphothreonineBy similarity
    Modified residuei988 – 9881PhosphoserineBy similarity
    Modified residuei1047 – 10471PhosphoserineBy similarity
    Modified residuei1050 – 10501PhosphothreonineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Interactioni

    Subunit structurei

    Interacts with atms/PAF1, but not with CycT.By similarity

    Protein-protein interaction databases

    STRINGi7260.FBpp0246442.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini212 – 533322USPAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C19 family.Curated
    Contains 1 USP domain.Curated

    Phylogenomic databases

    eggNOGiCOG5533.
    InParanoidiB4MLR8.
    KOiK11855.
    OMAiENEMDRG.
    OrthoDBiEOG7XDBF6.
    PhylomeDBiB4MLR8.

    Family and domain databases

    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view]
    PfamiPF00443. UCH. 1 hit.
    [Graphical view]
    PROSITEiPS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B4MLR8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPVSLAVCET TTTTTANVVN AALRESLLGG SRSGAAVAGS GSASSLISGA     50
    GGAGASAAND DDSSSTATAN TNLQNQIVAS AKRVLLAKIE YEEVENYNDS 100
    VLDNLKTKYV VIKSPTPAAA AAATNNGNSN SAGSKILGAN GHDNRQKQQN 150
    ANGGCSTPTT TTTQTSSSST SSSSIEHNNN PNELPKPKRV LYQRENIRIG 200
    WKQSERKWQI GAGMINVGNT CYLNSTLQAL FHIPALANWL NSEQSHLENC 250
    NIGGESGGGG GGGGGNNGGF CIICAMAKTL QSTQSNASAI RPYHIYSKLK 300
    QICKHMVIGR QEDAHEFLRF LVEAMEKAYL MRYRNYKELD QLVKETTPLN 350
    QIFGGYLRSE VRCLSCNHVS ITFQHFQDLL LDIRKSDTLD EAFDGYFSRE 400
    RLEDMGYKCE GCKKKVSATK QFSLQRAPIT LCIQLKRFSM IGNKLTKQIS 450
    FKPRIDLSRF AARSPTAQGQ LPLTYRLVSL VTHLGVSQHC GHYTAIGLTE 500
    SGSYYNFDDS YVRPIAMQSV CSTNSYIMFY ELDLNQPLAT PANKLNGLRQ 550
    LSNGHHHHQQ QQQQHQQQQQ QQPTVVATIA SSPMATTRFI GPQLPPGGLN 600
    GYAMTTTTTA TNNTTNGHSQ KTAIQFKPQQ NGILTVGSGK FQESGQQKSP 650
    LVGTNHKNEA PAVAPNANAN ANGKSSSNPN TNTTINTNTN NGNSNNNNTN 700
    ATTANNSNKL NQQQQQYLPM SSSDEEDSDE EKMKRPTTTT PQLPSMPKMD 750
    GGGDEKPKTL TLTPTTTPTA STPTVSNPLK RLVPYESASE EEESSSGTPS 800
    SSTPTTTTTA AAAAASSPMQ ATAAATLPPP PTPTNARKRS LPDHHHHHPH 850
    HHVMVNGHGK SPKPASMPPA TNFNSSSSKQ KTDAIDEIFK SLNNFNKKRI 900
    NNKNQKHNEG DEEEDDEETL EKETNNSSRL VSSSTNTSPT TNGWKQSQIV 950
    SSSSSNSKNV STSAAAAAAT TSSSTSTSAP PSPKTPPSPA VINSKTGLWK 1000
    VTRNLDDDDD EEEEDEDDEE HIAPTPPPVV AKTHKNPFSS QQKPTPSPST 1050
    EAAPKRQKLF NGTSSSTPHV GNGYQSEPST PNGGGSGSGS NGCLNELLKQ 1100
    SHRGYGSSSV LSWNGKQTEL DKEPFELVCA KRIAVDHGDH DDGGGGDDGG 1150
    GVGVVTTTTT TTTTTKNTTK TLTADAQEQR QRDLDDDEEN EMDRGRQRKV 1200
    KTATANGKSS GNSNNTTPGY NPFQEYESQK RWHNKSSNGP PRFYTQHASS 1250
    SYRSNFHQRN KFKCNRGGNG GGGSGGISKF DHRHGLQRHL AAGGGFPRRP 1300
    NANQQQQQQQ S 1311
    Length:1,311
    Mass (Da):141,002
    Last modified:September 23, 2008 - v1
    Checksum:i408909EBD862A2ED
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH963847 Genomic DNA. Translation: EDW72994.1.
    RefSeqiXP_002062008.1. XM_002061972.1.

    Genome annotation databases

    EnsemblMetazoaiFBtr0247950; FBpp0246442; FBgn0219298.
    GeneIDi6638806.
    KEGGidwi:Dwil_GK17299.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH963847 Genomic DNA. Translation: EDW72994.1 .
    RefSeqi XP_002062008.1. XM_002061972.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 7260.FBpp0246442.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0247950 ; FBpp0246442 ; FBgn0219298 .
    GeneIDi 6638806.
    KEGGi dwi:Dwil_GK17299.

    Organism-specific databases

    FlyBasei FBgn0219298. Dwil\GK17299.

    Phylogenomic databases

    eggNOGi COG5533.
    InParanoidi B4MLR8.
    KOi K11855.
    OMAi ENEMDRG.
    OrthoDBi EOG7XDBF6.
    PhylomeDBi B4MLR8.

    Family and domain databases

    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view ]
    Pfami PF00443. UCH. 1 hit.
    [Graphical view ]
    PROSITEi PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Evolution of genes and genomes on the Drosophila phylogeny."
      Drosophila 12 genomes consortium
      Nature 450:203-218(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Tucson 14030-0811.24.

    Entry informationi

    Entry nameiUBP36_DROWI
    AccessioniPrimary (citable) accession number: B4MLR8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 7, 2009
    Last sequence update: September 23, 2008
    Last modified: October 1, 2014
    This is version 33 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3