B4MA02 (UBE2S_DROVI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-conjugating enzyme E2 S
EC=6.3.2.19

Alternative name(s):
Ubiquitin carrier protein S
Ubiquitin-protein ligase S

Gene names

ORF Names:

GJ15767

Organism

Drosophila virilis (Fruit fly) [Complete proteome]

Taxonomic identifier

7244 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Drosophila › virilis group

Protein attributes

Sequence length	208 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating polyubiquitin chains initiated by the E2 enzyme vih/UbcH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Ubl conjugation pathway
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process Inferred from sequence or structural similarity. Source: UniProtKB cell division Inferred from electronic annotation. Source: UniProtKB-KW exit from mitosis Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 208	208	Ubiquitin-conjugating enzyme E2 S		PRO_0000390446
Sites
	Active site	98	1	Glycyl thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B4MA02 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 27A8093270FFC34B	FASTA	208	23,308
	10 20 30 40 50 60 MSSQYSNVEN LSPQTIRQVM KELQDMETTP PEGIKVLINE SDVTDIQAFI DGPAGTPYAV 70 80 90 100 110 120 GVFRVKLTLS KDFPQTPPKA YFLTKIFHPN VASNGEICVN TLKKDWKPDL GIKHILLTIK 130 140 150 160 170 180 CLLIVPNPES ALNEEAGKML LERYDDYSQR ARMMTEIHAQ PAKCGAGASD AKDDDGPSTK 190 200 KHAGLDKKLQ DKKKEKLLKE KKRMLKRL

References

[1]	"Evolution of genes and genomes on the Drosophila phylogeny." Drosophila 12 genomes consortium Nature 450:203-218(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tucson 15010-1051.87.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH940655 Genomic DNA. Translation: EDW66061.1.
RefSeq	XP_002057953.1. XM_002057917.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0231692; FBpp0230184; FBgn0202955.
GeneID	6634465.
KEGG	dvi:Dvir_GJ15767.
Organism-specific databases
FlyBase	FBgn0202955. Dvir\GJ15767.
Phylogenomic databases
eggNOG	COG5078.
InParanoid	B4MA02.
KO	K10583.
OMA	PDLGIKH.
OrthoDB	EOG4BNZV4.
Enzyme and pathway databases
UniPathway	UPA00143.
Family and domain databases
Gene3D	3.10.110.10. 1 hit.
InterPro	IPR000608. UBQ-conjugat_E2. IPR023313. UBQ-conjugating_AS. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBE2S_DROVI

Accession

Primary (citable) accession number: B4MA02

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2009
Last sequence update:	September 23, 2008
Last modified:	March 6, 2013
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project