B4M818 (T23O_DROVI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan 2,3-dioxygenase
Short name=TDO
EC=1.13.11.11

Alternative name(s):
Protein vermilion
Tryptamin 2,3-dioxygenase
Tryptophan oxygenase
Short name=TO
Short name=TRPO
Tryptophan pyrrolase
Tryptophanase

Gene names

Name:	v
ORF Names:	GJ16721

Organism

Drosophila virilis (Fruit fly) [Complete proteome]

Taxonomic identifier

7244 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila

Protein attributes

Sequence length	380 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring By similarity.
Catalytic activity	L-tryptophan + O2 = N-formyl-L-kynurenine.
Cofactor	Binds 2 heme groups per tetramer By similarity.
Pathway	Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2. Pigment biosynthesis; ommochrome biosynthesis.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the tryptophan 2,3-dioxygenase family.
Sequence caution	The sequence EDW62294.1 differs from that shown. Reason: Frameshift at positions 236 and 241.

Ontologies

Keywords
Biological process	Tryptophan catabolism
Ligand	Heme Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tryptophan catabolic process to kynurenine Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	heme binding Inferred from sequence or structural similarity. Source: UniProtKB tryptophan 2,3-dioxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 380	380	Tryptophan 2,3-dioxygenase		PRO_0000360882
Regions
	Region	27 – 31	5	Substrate binding By similarity
	Region	57 – 61	5	Substrate binding By similarity
Sites
	Metal binding	313	1	Iron (heme axial ligand) By similarity
	Binding site	128	1	Substrate By similarity
	Binding site	135	1	Heme By similarity
	Binding site	328	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B4M818 [UniParc]. Last modified January 20, 2009. Version 2. Checksum: 2CCE428FA8511436	FASTA	380	44,357
	10 20 30 40 50 60 MSCPYAGNGN EHDDGAVPLS NEVGKIYGEY LMLDKLLDAQ CMLSMEDKRP VHDEHLFIIT 70 80 90 100 110 120 HQAYELWFKQ IIFEFDSIRV MLDEEVIDET KTLEIVKRLN RVVLILKLLV DQVPILETMT 130 140 150 160 170 180 PLDFMDFRKY LAPASGFQSL QFRLIENKLG VLTEQRVKYN QKYSDVFGND ERALHAIRSS 190 200 210 220 230 240 EDGPSLLVLV QRWLERTPGL EEEGFNFWSK FQQSVDQFLA AQVQSALLEP VEWAKNYRLM 250 260 270 280 290 300 DIEKRREVYR SIFDPAVHEA LVKRGDRRFS HRALQGAIMI TFYRDEPRFS QPHQLLTLLM 310 320 330 340 350 360 DIDSLITKWR YNHVIMVQRM IGSQQLGTGG SSGYQYLRST LSDRYKVFLD LFNLSTFLIP 370 380 REAIPPLDET IRKKLVHKSV

References

[1]	"Evolution of genes and genomes on the Drosophila phylogeny." Drosophila 12 genomes consortium Nature 450:203-218(2007) [PubMed: 17994087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tucson 15010-1051.87.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH940653 Genomic DNA. Translation: EDW62294.1. Frameshift.
RefSeq	XP_002056808.1. XM_002056772.1.
3D structure databases
ProteinModelPortal	B4M818.
ModBase	Search...
Protein-protein interaction databases
STRING	B4M818.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6633596.
KEGG	dvi:Dvir_GJ16721.
Organism-specific databases
FlyBase	FBgn0203902. Dvir\GJ16721.
Phylogenomic databases
GeneTree	EMGT00050000008199.
InParanoid	B4M818.
OrthoDB	EOG4NCJVC.
Family and domain databases
InterPro	IPR004981. Trp_2_3_dOase. [Graphical view]
KO	K00453.
PANTHER	PTHR10138. Trp_2_3_dOase. 1 hit.
Pfam	PF03301. Trp_dioxygenase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T23O_DROVI

Accession

Primary (citable) accession number: B4M818

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	January 20, 2009
Last modified:	January 25, 2012
This is version 25 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project