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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

GJ16889

Organism
Drosophila virilis (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei70S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei148S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Active sitei151UniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase, tRNA-binding
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
ORF Names:GJ16889
OrganismiDrosophila virilis (Fruit fly)
Taxonomic identifieri7244 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophila
Proteomesi
  • UP000008792 Componenti: Unassembled WGS sequence

Organism-specific databases

FlyBaseiFBgn0204068. Dvir\GJ16889.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003705761 – 247tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST247

Expressioni

Gene expression databases

BgeeiFBgn0204068.

Interactioni

Protein-protein interaction databases

STRINGi7244.FBpp0231306.

Structurei

3D structure databases

SMRiB4M703.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni93 – 94S-adenosyl-L-methionine bindingUniRule annotation2
Regioni128 – 129S-adenosyl-L-methionine bindingUniRule annotation2
Regioni226 – 228S-adenosyl-L-methionine bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiKOG3115. Eukaryota.
COG0220. LUCA.
InParanoidiB4M703.
KOiK03439.
OMAiIGMEIRV.
OrthoDBiEOG091G0IQ7.
PhylomeDBiB4M703.

Family and domain databases

HAMAPiMF_03055. tRNA_methyltr_TrmB_euk. 1 hit.
InterProiView protein in InterPro
IPR029063. SAM-dependent_MTases.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
PANTHERiPTHR23417. PTHR23417. 1 hit.
PTHR23417:SF20. PTHR23417:SF20. 1 hit.
PfamiView protein in Pfam
PF02390. Methyltransf_4. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiView protein in PROSITE
PS51625. SAM_MT_TRMB. 1 hit.

Sequencei

Sequence statusi: Complete.

B4M703-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTNSQEGET LPATSSVTGL PQKRFYRQRA HSNPIADHSF DYPARPEDVD
60 70 80 90 100
WRSLYPNIGS EQQVEFADIG CGYGGFLVTL GEMFPDKLAI GMEIRVKVSD
110 120 130 140 150
YVIDRIAALR MKNAETSAYQ NIACIRTNAM KYLPNYFQKS QLEKMFFLYP
160 170 180 190 200
DPHFKRAKHK WRIINQALLS EYAYVLRSGG LVYTMTDVED LHKWIVSHMT
210 220 230 240
QHPLYERLTD EEANADPITP KLYQSSEEGA KVVRNKGDHF LAIFRRI
Length:247
Mass (Da):28,463
Last modified:September 23, 2008 - v1
Checksum:i225A7A97B192FD46
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH940653 Genomic DNA. Translation: EDW62570.1.
RefSeqiXP_002057084.1. XM_002057048.2.

Genome annotation databases

EnsemblMetazoaiFBtr0232814; FBpp0231306; FBgn0204068.
GeneIDi6633719.
KEGGidvi:Dvir_GJ16889.

Similar proteinsi

Entry informationi

Entry nameiTRMB_DROVI
AccessioniPrimary (citable) accession number: B4M703
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: September 23, 2008
Last modified: August 30, 2017
This is version 52 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families