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B4M703 (TRMB_DROVI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
ORF Names:GJ16889
OrganismDrosophila virilis (Fruit fly) [Complete proteome]
Taxonomic identifier7244 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophila

Protein attributes

Sequence length247 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_03055

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_03055

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_03055

Subcellular location

Nucleus By similarity HAMAP-Rule MF_03055.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentNucleus
   LigandRNA-binding
S-adenosyl-L-methionine
tRNA-binding
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 247247tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_03055
PRO_0000370576

Regions

Region93 – 942S-adenosyl-L-methionine binding By similarity
Region128 – 1292S-adenosyl-L-methionine binding By similarity
Region226 – 2283S-adenosyl-L-methionine binding By similarity

Sites

Active site1511 By similarity
Binding site701S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site1481S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
B4M703 [UniParc].

Last modified September 23, 2008. Version 1.
Checksum: 225A7A97B192FD46

FASTA24728,463
        10         20         30         40         50         60 
MVTNSQEGET LPATSSVTGL PQKRFYRQRA HSNPIADHSF DYPARPEDVD WRSLYPNIGS 

        70         80         90        100        110        120 
EQQVEFADIG CGYGGFLVTL GEMFPDKLAI GMEIRVKVSD YVIDRIAALR MKNAETSAYQ 

       130        140        150        160        170        180 
NIACIRTNAM KYLPNYFQKS QLEKMFFLYP DPHFKRAKHK WRIINQALLS EYAYVLRSGG 

       190        200        210        220        230        240 
LVYTMTDVED LHKWIVSHMT QHPLYERLTD EEANADPITP KLYQSSEEGA KVVRNKGDHF 


LAIFRRI 

« Hide

References

[1]"Evolution of genes and genomes on the Drosophila phylogeny."
Drosophila 12 genomes consortium
Nature 450:203-218(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Tucson 15010-1051.87.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CH940653 Genomic DNA. Translation: EDW62570.1.
RefSeqXP_002057084.1. XM_002057048.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING7244.FBpp0231306.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0232814; FBpp0231306; FBgn0204068.
GeneID6633719.
KEGGdvi:Dvir_GJ16889.

Organism-specific databases

FlyBaseFBgn0204068. Dvir\GJ16889.

Phylogenomic databases

eggNOGCOG0220.
InParanoidB4M703.
KOK03439.
OMARAHSNPI.
OrthoDBEOG7PS1GC.
PhylomeDBB4M703.

Enzyme and pathway databases

UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_03055. tRNA_methyltr_TrmB_euk.
InterProIPR029063. SAM-dependent_MTases-like.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_DROVI
AccessionPrimary (citable) accession number: B4M703
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: September 23, 2008
Last modified: July 9, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase