Reviewed, UniProtKB/Swiss-Prot B4M0H8 (SPAST_DROVI)

Names and origin

Protein names

Recommended name:
    Spastin
    EC=3.6.4.3

Gene names

Name:	spas
ORF Names:	GJ24660

Organism

Drosophila virilis (Fruit fly)

Taxonomic identifier

7244 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila

Protein attributes

Sequence length	769 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	ATP-dependent microtubule severing protein. Stimulates microtubule minus-end depolymerization and poleward microtubule flux in the mitotic spindle. Regulates microtubule stability in the neuromuscular junction synapse By similarity.
Catalytic activity	ATP + H2O = ADP + phosphate.
Subunit structure	Homohexamer. The homohexamer is stabilized by ATP-binding. The homohexamer may adopt a ring conformation through which microtubules pass prior to being severed. Interacts with microtubules By similarity.
Subcellular location	Membrane; Single-pass membrane protein Potential. Cytoplasm › cytoskeleton › centrosome By similarity. Cytoplasm › cytoskeleton By similarity. Note: Colocalizes with cellular microtubule arrays. Localizes to chromosomes from prometaphase/metaphase to anaphase, and this requires microtubules. Localizes to discrete punctate cytoplasmic foci which may correspond to secretory vesicles By similarity.
Sequence similarities	Belongs to the AAA ATPase family. Spastin subfamily. Contains 1 MIT domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Differentiation Mitosis Neurogenesis
Cellular component	Cytoplasm Cytoskeleton Membrane Microtubule
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Developmental protein Hydrolase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW microtubule severing Inferred from sequence or structural similarity. Source: UniProtKB mitosis Inferred from electronic annotation. Source: UniProtKB-KW nervous system development Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	centrosome Inferred from sequence or structural similarity. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microtubule Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW microtubule binding Inferred from sequence or structural similarity. Source: UniProtKB microtubule-severing ATPase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 769	769	Spastin		PRO_0000367150
Regions
	Transmembrane	107 – 126	20	Potential
	Domain	223 – 299	77	MIT
	Nucleotide binding	534 – 541	8	ATP Potential
	Region	1 – 199	199	Required for localization to punctate cytoplasmic foci By similarity
	Region	196 – 769	574	Sufficient for interaction with microtubules and microtubule severing By similarity
	Region	452 – 466	15	Required for interaction with microtubules By similarity
Amino acid modifications
	Modified residue	448	1	Phosphothreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B4M0H8-1 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 9397CC16A608C9E2	FASTA	769	83,232
	10 20 30 40 50 60 MVRTKNQSSS SSASSSTKSP VKISGGGGGG GGSSSSTNRS RSCSEALIDD GKSSSKLSSN 70 80 90 100 110 120 RQRTTTTITT TTTTPGSSPD DDTTDADLTP TSGNVPRGGQ SVHKQNLYVV SFPIIFLFNV 130 140 150 160 170 180 LRSLIYQLFC IFRYLYGAST KVIYRSPHRR DCNIEIVVQN SKEQQAIICP LEGSGVNIEQ 190 200 210 220 230 240 AQILPQRQRA LQTLEMAASR GGTGAGGYSP GPGDPLLAKQ KHHHRRAFEY ISKALKIDEE 250 260 270 280 290 300 NEGHKELAIE LYRKGIKELE DGIAVDCWSG RGDVWDRAQR LHEKMQTNLS MARDRLHFLA 310 320 330 340 350 360 LREEDLQMQR LSLKEQPKKQ LPHKFKQPML VGQTTTSSGS SSSSRASAEP PKITLRSSGY 370 380 390 400 410 420 GPKTGGATTS KAVPAASGRK LTIGNKRPGN LAVANKSQTL PRNLGSKTTS TSVGAALQRQ 430 440 450 460 470 480 PGKTAATPPA VRRQFSSGRN TPPQRSRTPI NNNAASGSGS GASTPLISVK GVEQKLVQLI 490 500 510 520 530 540 LDEIVEGGAK VEWSDIAGQD VAKQALQEMV ILPSVRPELF TGLRAPAKGL LLFGPPGNGK 550 560 570 580 590 600 TLLARAVATE CSATFLNISA ASLTSKYVGD GEKLVRALFA VARHMQPSII FIDEVDSLLS 610 620 630 640 650 660 ERSSNEHEAS RRLKTEFLVE FDGLPGNPEG DRIVVLAATN RPQELDEAAL RRFTKRVYVS 670 680 690 700 710 720 LPGVQTRELL LSRLLQKQGS PLDTEALARL AKITDGYSGS DLTALAKDAA LEPIRELNVE 730 740 750 760 QVKCLDISAM RPITEKDFHN SLKRIRRSVA PQSLNSYEKW SQDYGDITI

References

[1]	"Evolution of genes and genomes on the Drosophila phylogeny." Drosophila 12 genomes consortium Nature 450:203-218(2007) [PubMed: 17994087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tucson 15010-1051.87.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CH940650 Genomic DNA. Translation: EDW68357.1.
RefSeq	XP_002054837.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6631192.
KEGG	dvi:Dvir_GJ24660.
Organism-specific databases
FlyBase	FBgn0211737. Dvir\GJ24660.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR003960. ATPase_AAA_CS. IPR007330. MIT. [Graphical view]
Pfam	PF00004. AAA. 1 hit. PF04212. MIT. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. SM00745. MIT. 1 hit. [Graphical view]
PROSITE	PS00674. AAA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPAST_DROVI

Accession

Primary (citable) accession number: B4M0H8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 23, 2008
Last modified:	October 13, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project