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Protein

cGMP-specific 3',5'-cyclic phosphodiesterase

Gene

Pde6

Organism
Drosophila virilis (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Has a role regulating cGMP transport in Malpighian tubule principal cells.By similarity

Catalytic activityi

Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.By similarity

Cofactori

a divalent metal cationBy similarityNote: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei496 – 4961Proton donorBy similarity
Metal bindingi500 – 5001Divalent metal cation 1By similarity
Metal bindingi536 – 5361Divalent metal cation 1By similarity
Metal bindingi537 – 5371Divalent metal cation 1By similarity
Metal bindingi537 – 5371Divalent metal cation 2By similarity
Metal bindingi647 – 6471Divalent metal cation 1By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

cGMP, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
cGMP-specific 3',5'-cyclic phosphodiesteraseBy similarity (EC:3.1.4.35)
Gene namesi
Name:Pde6By similarity
ORF Names:GJ23019
OrganismiDrosophila virilis (Fruit fly)
Taxonomic identifieri7244 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophila
ProteomesiUP000008792 Componenti: Unassembled WGS sequence

Organism-specific databases

FlyBaseiFBgn0210121. Dvir\GJ23019.

Subcellular locationi

  • Cell membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 890890cGMP-specific 3',5'-cyclic phosphodiesterasePRO_0000363700Add
BLAST
Propeptidei891 – 8933Removed in mature formBy similarityPRO_0000363701

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei890 – 8901Cysteine methyl esterBy similarity
Lipidationi890 – 8901S-farnesyl cysteineBy similarity

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Interactioni

Subunit structurei

Interacts with PrBP.By similarity

Protein-protein interaction databases

STRINGi7244.FBpp0237436.

Structurei

3D structure databases

ProteinModelPortaliB4LVU6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 173153GAF 1Sequence AnalysisAdd
BLAST
Domaini205 – 390186GAF 2Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the cyclic nucleotide phosphodiesterase family.Sequence Analysis
Contains 2 GAF domains.Sequence Analysis

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG270709.
InParanoidiB4LVU6.
KOiK13763.
OMAiDDNPSNP.
OrthoDBiEOG7RRF69.
PhylomeDBiB4LVU6.

Family and domain databases

Gene3Di1.10.1300.10. 1 hit.
3.30.450.40. 3 hits.
InterProiIPR003018. GAF.
IPR029016. GAF_dom_like.
IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamiPF01590. GAF. 2 hits.
PF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00065. GAF. 2 hits.
SM00471. HDc. 1 hit.
[Graphical view]
SUPFAMiSSF55781. SSF55781. 3 hits.
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B4LVU6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELDEGELFM ELIRDVANEL DIDVLCHKIL VNVGLLTHAD RGSLFLAKGT
60 70 80 90 100
PNNKYLVAKL FDVTQKTALK DAVTRARAEE IIIPFGIGIA GMVAQTKEMI
110 120 130 140 150
NIKEAYMDAR FNCEIDLKTG YKTNAILCMP ICNYEGDIIG VAQIINKTNG
160 170 180 190 200
CMEFDEHDVE IFRRYLTFCG IGIQNAQLFE MSVQEYRRNQ ILLNLARSIF
210 220 230 240 250
EEQNNLECLV TKIMTEAREL LKCERCSVFL VDLDCCEESH LEKIIEKPHQ
260 270 280 290 300
LEQRATRAIK GGDSFEEKQK MRNRFTVLFE LGGESQAANV SRPSINDLSH
310 320 330 340 350
STLAQIAQFV ATTGQTVNIC DVHDWVREHN QIRAEGEIDS THAILCMPIV
360 370 380 390 400
NAQKTVIGVA QLINKASGLP FTESDASIFE AFAIFCGLGI HNTQMYENAC
410 420 430 440 450
KLMAKQKVAL ECLSYHATAG QDQTEKLIQD PIAEAETYNL YSFTFTDFDL
460 470 480 490 500
VDDDTCRAVL RMFMQCNLVS QFHIPYDVLC RWVLSVRKNY RPVKYHNWRH
510 520 530 540 550
ALNVAQTMFA MLKTGKMERF MTDLEILGLL VACLCHDLDH RGTNNAFQTK
560 570 580 590 600
TESPLAILYT TSTMEHHHFD QCVMILNSEG NNIFQALSPE DYRSVMKTVE
610 620 630 640 650
SAILSTDLAM YFKKRNAFLE LVENGEFDWQ GEEKKDLLCG MMMTACDVSA
660 670 680 690 700
IAKPWEVQHR VAKLVADEFF DQGDLEKLQL NTQPVAMMDR ERKDELPKMQ
710 720 730 740 750
VGFIDVICLP LYRVLCDTFP WITPLYEGTL ENRRNWQDLA EKVEMGLTWI
760 770 780 790 800
DHDTIDKPVE EFAGCADEEI KDIEFTVTTL NCNQQSQHGG DDSHTPEHQR
810 820 830 840 850
SGSRLSIKKT GALGKVVRSK LSKTLYNSMD GSKPKTSLKL LESHVSEDMD
860 870 880 890
DKSPTSPSQP HLGSVGRMSA SSSTSSAGTV DKSKKRSKLC ALL
Length:893
Mass (Da):100,943
Last modified:September 23, 2008 - v1
Checksum:i2CD8AD4CCE687DDE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH940650 Genomic DNA. Translation: EDW67551.1.
RefSeqiXP_002054031.1. XM_002053995.1.

Genome annotation databases

EnsemblMetazoaiFBtr0238944; FBpp0237436; FBgn0210121.
GeneIDi6630351.
KEGGidvi:Dvir_GJ23019.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH940650 Genomic DNA. Translation: EDW67551.1.
RefSeqiXP_002054031.1. XM_002053995.1.

3D structure databases

ProteinModelPortaliB4LVU6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7244.FBpp0237436.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0238944; FBpp0237436; FBgn0210121.
GeneIDi6630351.
KEGGidvi:Dvir_GJ23019.

Organism-specific databases

FlyBaseiFBgn0210121. Dvir\GJ23019.

Phylogenomic databases

eggNOGiNOG270709.
InParanoidiB4LVU6.
KOiK13763.
OMAiDDNPSNP.
OrthoDBiEOG7RRF69.
PhylomeDBiB4LVU6.

Family and domain databases

Gene3Di1.10.1300.10. 1 hit.
3.30.450.40. 3 hits.
InterProiIPR003018. GAF.
IPR029016. GAF_dom_like.
IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamiPF01590. GAF. 2 hits.
PF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00065. GAF. 2 hits.
SM00471. HDc. 1 hit.
[Graphical view]
SUPFAMiSSF55781. SSF55781. 3 hits.
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Evolution of genes and genomes on the Drosophila phylogeny."
    Drosophila 12 genomes consortium
    Nature 450:203-218(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Tucson 15010-1051.87Imported.

Entry informationi

Entry nameiPDE6_DROVI
AccessioniPrimary (citable) accession number: B4LVU6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: September 23, 2008
Last modified: January 7, 2015
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.