Reviewed, UniProtKB/Swiss-Prot B4LVU6 (PDE6_DROVI)

Names and origin

Protein names

Recommended name:
    cGMP-specific 3',5'-cyclic phosphodiesterase
    EC=3.1.4.35

Gene names

Name:	Pde6
ORF Names:	GJ23019

Organism

Drosophila virilis (Fruit fly)

Taxonomic identifier

7244 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila

Protein attributes

Sequence length	893 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Has a role regulating cGMP transport in Malpighian tubule principal cells By similarity. UniProtKB Q9VFI9
Catalytic activity	Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate. UniProtKB Q9VFI9
Subunit structure	Interacts with PrBP By similarity. UniProtKB Q9VFI9
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side By similarity. UniProtKB Q9VFI9
Sequence similarities	Belongs to the cyclic nucleotide phosphodiesterase family. Contains 2 GAF domains.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Repeat
Ligand	cGMP
Molecular function	Hydrolase
PTM	Lipoprotein Methylation Prenylation
Gene Ontology (GO)
Biological process	cGMP metabolic process Inferred from sequence or structural similarity. Source: UniProtKB signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	internal side of plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3',5'-cyclic-GMP phosphodiesterase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 890	890	cGMP-specific 3',5'-cyclic phosphodiesterase UniProtKB Q9VFI9		PRO_0000363700
	Propeptide	891 – 893	3	Removed in mature form By similarity UniProtKB Q9VFI9		PRO_0000363701
Regions
	Domain	21 – 173	153	GAF 1
	Domain	205 – 390	186	GAF 2
Amino acid modifications
	Modified residue	890	1	Cysteine methyl ester By similarity UniProtKB Q9VFI9
	Lipidation	890	1	S-farnesyl cysteine By similarity UniProtKB Q9VFI9

Sequences

	Sequence		Length	Mass (Da)
	B4LVU6-1 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 2CD8AD4CCE687DDE	FASTA	893	100,943
	10 20 30 40 50 60 MELDEGELFM ELIRDVANEL DIDVLCHKIL VNVGLLTHAD RGSLFLAKGT PNNKYLVAKL 70 80 90 100 110 120 FDVTQKTALK DAVTRARAEE IIIPFGIGIA GMVAQTKEMI NIKEAYMDAR FNCEIDLKTG 130 140 150 160 170 180 YKTNAILCMP ICNYEGDIIG VAQIINKTNG CMEFDEHDVE IFRRYLTFCG IGIQNAQLFE 190 200 210 220 230 240 MSVQEYRRNQ ILLNLARSIF EEQNNLECLV TKIMTEAREL LKCERCSVFL VDLDCCEESH 250 260 270 280 290 300 LEKIIEKPHQ LEQRATRAIK GGDSFEEKQK MRNRFTVLFE LGGESQAANV SRPSINDLSH 310 320 330 340 350 360 STLAQIAQFV ATTGQTVNIC DVHDWVREHN QIRAEGEIDS THAILCMPIV NAQKTVIGVA 370 380 390 400 410 420 QLINKASGLP FTESDASIFE AFAIFCGLGI HNTQMYENAC KLMAKQKVAL ECLSYHATAG 430 440 450 460 470 480 QDQTEKLIQD PIAEAETYNL YSFTFTDFDL VDDDTCRAVL RMFMQCNLVS QFHIPYDVLC 490 500 510 520 530 540 RWVLSVRKNY RPVKYHNWRH ALNVAQTMFA MLKTGKMERF MTDLEILGLL VACLCHDLDH 550 560 570 580 590 600 RGTNNAFQTK TESPLAILYT TSTMEHHHFD QCVMILNSEG NNIFQALSPE DYRSVMKTVE 610 620 630 640 650 660 SAILSTDLAM YFKKRNAFLE LVENGEFDWQ GEEKKDLLCG MMMTACDVSA IAKPWEVQHR 670 680 690 700 710 720 VAKLVADEFF DQGDLEKLQL NTQPVAMMDR ERKDELPKMQ VGFIDVICLP LYRVLCDTFP 730 740 750 760 770 780 WITPLYEGTL ENRRNWQDLA EKVEMGLTWI DHDTIDKPVE EFAGCADEEI KDIEFTVTTL 790 800 810 820 830 840 NCNQQSQHGG DDSHTPEHQR SGSRLSIKKT GALGKVVRSK LSKTLYNSMD GSKPKTSLKL 850 860 870 880 890 LESHVSEDMD DKSPTSPSQP HLGSVGRMSA SSSTSSAGTV DKSKKRSKLC ALL

References

[1]	"Evolution of genes and genomes on the Drosophila phylogeny." Drosophila 12 genomes consortium Nature 450:203-218(2007) [PubMed: 17994087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tucson 15010-1051.87.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CH940650 Genomic DNA. Translation: EDW67551.1.
RefSeq	XP_002054031.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6630351.
KEGG	dvi:Dvir_GJ23019.
Organism-specific databases
FlyBase	FBgn0210121. Dvir\GJ23019.
Family and domain databases
InterPro	IPR003018. GAF. IPR003607. Met-dep_phosphohydro_HD. IPR002073. PDEase. [Graphical view]
Pfam	PF01590. GAF. 2 hits. PF00233. PDEase_I. 1 hit. [Graphical view]
PRINTS	PR00387. PDIESTERASE1.
SMART	SM00065. GAF. 2 hits. SM00471. HDc. 1 hit. [Graphical view]
PROSITE	PS00126. PDEASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PDE6_DROVI

Accession

Primary (citable) accession number: B4LVU6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	September 23, 2008
Last modified:	October 13, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project