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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

GI21651

Organism
Drosophila mojavensis (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei70S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei149S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Active sitei152UniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase, tRNA-binding
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
ORF Names:GI21651
OrganismiDrosophila mojavensis (Fruit fly)
Taxonomic identifieri7230 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophila
Proteomesi
  • UP000009192 Componenti: Unassembled WGS sequence

Organism-specific databases

FlyBaseiFBgn0144381. Dmoj\GI21651.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003705711 – 248tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST248

Expressioni

Gene expression databases

BgeeiFBgn0144381.

Structurei

3D structure databases

SMRiB4L529.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni93 – 94S-adenosyl-L-methionine bindingUniRule annotation2
Regioni129 – 130S-adenosyl-L-methionine bindingUniRule annotation2
Regioni227 – 229S-adenosyl-L-methionine bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

InParanoidiB4L529.
KOiK03439.
OMAiIGMEIRV.
OrthoDBiEOG091G0IQ7.
PhylomeDBiB4L529.

Family and domain databases

HAMAPiMF_03055. tRNA_methyltr_TrmB_euk. 1 hit.
InterProiView protein in InterPro
IPR029063. SAM-dependent_MTases.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
PANTHERiPTHR23417. PTHR23417. 1 hit.
PTHR23417:SF16. PTHR23417:SF16. 1 hit.
PfamiView protein in Pfam
PF02390. Methyltransf_4. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiView protein in PROSITE
PS51625. SAM_MT_TRMB. 1 hit.

Sequencei

Sequence statusi: Complete.

B4L529-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVANSKESET LTGASAVTGL PQKRFYRQRA HSNPIADHSF NYPARPEDVD
60 70 80 90 100
WRSLYPSMGD DQQVQFADIG CGYGGFLVTL GEMFPEKLAI GMEIRVKVSD
110 120 130 140 150
YVIDRIAALR LKNANEATTY QNIACIRTNA MKYLPNYFQK SQLEKMFFLY
160 170 180 190 200
PDPHFKRAKH KWRIINQALL SEYAYVLRKG GLVYTMTDVE DLHTWIVSHM
210 220 230 240
TQHPLFERLS DEEANADPIT PKLYQSSEEG AKVVRNKGEH FLAIFRRI
Length:248
Mass (Da):28,533
Last modified:September 23, 2008 - v1
Checksum:i66AC853EF0001880
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH933811 Genomic DNA. Translation: EDW06288.1.
RefSeqiXP_002010633.1. XM_002010597.2.

Genome annotation databases

EnsemblMetazoaiFBtr0172376; FBpp0170868; FBgn0144381.
GeneIDi6584993.
KEGGidmo:Dmoj_GI21651.

Similar proteinsi

Entry informationi

Entry nameiTRMB_DROMO
AccessioniPrimary (citable) accession number: B4L529
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: September 23, 2008
Last modified: October 25, 2017
This is version 46 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families