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B4L529

- TRMB_DROMO

UniProt

B4L529 - TRMB_DROMO

Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

GI21651

Organism
Drosophila mojavensis (Fruit fly)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 34 (01 Oct 2014)
      Sequence version 1 (23 Sep 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

    Catalytic activityi

    S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei70 – 701S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
    Binding sitei149 – 1491S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
    Active sitei152 – 1521UniRule annotation

    GO - Molecular functioni

    1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
    2. tRNA binding Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    RNA-binding, S-adenosyl-L-methionine, tRNA-binding

    Enzyme and pathway databases

    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
    tRNA(m7G46)-methyltransferaseUniRule annotation
    Gene namesi
    ORF Names:GI21651
    OrganismiDrosophila mojavensis (Fruit fly)
    Taxonomic identifieri7230 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophila
    ProteomesiUP000009192: Unassembled WGS sequence

    Organism-specific databases

    FlyBaseiFBgn0144381. Dmoj\GI21651.

    Subcellular locationi

    Nucleus UniRule annotation

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 248248tRNA (guanine-N(7)-)-methyltransferasePRO_0000370571Add
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni93 – 942S-adenosyl-L-methionine bindingUniRule annotation
    Regioni129 – 1302S-adenosyl-L-methionine bindingUniRule annotation
    Regioni227 – 2293S-adenosyl-L-methionine bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

    Phylogenomic databases

    InParanoidiB4L529.
    KOiK03439.
    OrthoDBiEOG7PS1GC.
    PhylomeDBiB4L529.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR025763. Trm8_euk.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B4L529-1 [UniParc]FASTAAdd to Basket

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    MVANSKESET LTGASAVTGL PQKRFYRQRA HSNPIADHSF NYPARPEDVD    50
    WRSLYPSMGD DQQVQFADIG CGYGGFLVTL GEMFPEKLAI GMEIRVKVSD 100
    YVIDRIAALR LKNANEATTY QNIACIRTNA MKYLPNYFQK SQLEKMFFLY 150
    PDPHFKRAKH KWRIINQALL SEYAYVLRKG GLVYTMTDVE DLHTWIVSHM 200
    TQHPLFERLS DEEANADPIT PKLYQSSEEG AKVVRNKGEH FLAIFRRI 248
    Length:248
    Mass (Da):28,533
    Last modified:September 23, 2008 - v1
    Checksum:i66AC853EF0001880
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH933811 Genomic DNA. Translation: EDW06288.1.
    RefSeqiXP_002010633.1. XM_002010597.1.

    Genome annotation databases

    EnsemblMetazoaiFBtr0172376; FBpp0170868; FBgn0144381.
    GeneIDi6584993.
    KEGGidmo:Dmoj_GI21651.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH933811 Genomic DNA. Translation: EDW06288.1 .
    RefSeqi XP_002010633.1. XM_002010597.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0172376 ; FBpp0170868 ; FBgn0144381 .
    GeneIDi 6584993.
    KEGGi dmo:Dmoj_GI21651.

    Organism-specific databases

    FlyBasei FBgn0144381. Dmoj\GI21651.

    Phylogenomic databases

    InParanoidi B4L529.
    KOi K03439.
    OrthoDBi EOG7PS1GC.
    PhylomeDBi B4L529.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_03055. tRNA_methyltr_TrmB_euk.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR025763. Trm8_euk.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Evolution of genes and genomes on the Drosophila phylogeny."
      Drosophila 12 genomes consortium
      Nature 450:203-218(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Tucson 15081-1352.22.

    Entry informationi

    Entry nameiTRMB_DROMO
    AccessioniPrimary (citable) accession number: B4L529
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 5, 2009
    Last sequence update: September 23, 2008
    Last modified: October 1, 2014
    This is version 34 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3