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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

GI21651

Organism
Drosophila mojavensis (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathway:iN(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei70 – 701S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei149 – 1491S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Active sitei152 – 1521UniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine, tRNA-binding

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
ORF Names:GI21651
OrganismiDrosophila mojavensis (Fruit fly)
Taxonomic identifieri7230 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophila
ProteomesiUP000009192 Componenti: Unassembled WGS sequence

Organism-specific databases

FlyBaseiFBgn0144381. Dmoj\GI21651.

Subcellular locationi

  • Nucleus UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 248248tRNA (guanine-N(7)-)-methyltransferasePRO_0000370571Add
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni93 – 942S-adenosyl-L-methionine bindingUniRule annotation
Regioni129 – 1302S-adenosyl-L-methionine bindingUniRule annotation
Regioni227 – 2293S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

InParanoidiB4L529.
KOiK03439.
OMAiIGMEIRV.
OrthoDBiEOG7PS1GC.
PhylomeDBiB4L529.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
InterProiIPR029063. SAM-dependent_MTases.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B4L529-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVANSKESET LTGASAVTGL PQKRFYRQRA HSNPIADHSF NYPARPEDVD
60 70 80 90 100
WRSLYPSMGD DQQVQFADIG CGYGGFLVTL GEMFPEKLAI GMEIRVKVSD
110 120 130 140 150
YVIDRIAALR LKNANEATTY QNIACIRTNA MKYLPNYFQK SQLEKMFFLY
160 170 180 190 200
PDPHFKRAKH KWRIINQALL SEYAYVLRKG GLVYTMTDVE DLHTWIVSHM
210 220 230 240
TQHPLFERLS DEEANADPIT PKLYQSSEEG AKVVRNKGEH FLAIFRRI
Length:248
Mass (Da):28,533
Last modified:September 23, 2008 - v1
Checksum:i66AC853EF0001880
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH933811 Genomic DNA. Translation: EDW06288.1.
RefSeqiXP_002010633.1. XM_002010597.1.

Genome annotation databases

EnsemblMetazoaiFBtr0172376; FBpp0170868; FBgn0144381.
GeneIDi6584993.
KEGGidmo:Dmoj_GI21651.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH933811 Genomic DNA. Translation: EDW06288.1.
RefSeqiXP_002010633.1. XM_002010597.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0172376; FBpp0170868; FBgn0144381.
GeneIDi6584993.
KEGGidmo:Dmoj_GI21651.

Organism-specific databases

FlyBaseiFBgn0144381. Dmoj\GI21651.

Phylogenomic databases

InParanoidiB4L529.
KOiK03439.
OMAiIGMEIRV.
OrthoDBiEOG7PS1GC.
PhylomeDBiB4L529.

Enzyme and pathway databases

UniPathwayiUPA00989.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
InterProiIPR029063. SAM-dependent_MTases.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Evolution of genes and genomes on the Drosophila phylogeny."
    Drosophila 12 genomes consortium
    Nature 450:203-218(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Tucson 15081-1352.22.

Entry informationi

Entry nameiTRMB_DROMO
AccessioniPrimary (citable) accession number: B4L529
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: September 23, 2008
Last modified: July 22, 2015
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.