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B4KYY0

- LIAS_DROMO

UniProt

B4KYY0 - LIAS_DROMO

Protein

Lipoyl synthase, mitochondrial

Gene

Las

Organism
Drosophila mojavensis (Fruit fly)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 36 (01 Oct 2014)
      Sequence version 1 (23 Sep 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi99 – 991Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi104 – 1041Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi110 – 1101Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi130 – 1301Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi134 – 1341Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi137 – 1371Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lipoate synthaseUniRule annotation
    Short name:
    LSUniRule annotation
    Short name:
    Lip-synUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Gene namesi
    Name:LasUniRule annotation
    ORF Names:GI13471
    OrganismiDrosophila mojavensis (Fruit fly)
    Taxonomic identifieri7230 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophila
    ProteomesiUP000009192: Unassembled WGS sequence

    Organism-specific databases

    FlyBaseiFBgn0136227. Dmoj\GI13471.

    Subcellular locationi

    Mitochondrion UniRule annotation

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 364Lipoyl synthase, mitochondrialPRO_0000398220
    Transit peptidei1 – ?MitochondrionUniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliB4KYY0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    InParanoidiB4KYY0.
    KOiK03644.
    OrthoDBiEOG7P2XS7.
    PhylomeDBiB4KYY0.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    B4KYY0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLRKLNTPTT TLVRAASTRV EKLEEIRERL SKGPNFQDFI QNSDGVKEDW    50
    ENYDGKLRRE KGETQQLRLP PWLKTTIPMG KNYAKIKSQL RDLKLSTVCE 100
    EARCPNIGEC WGGGEHSTQT ATIMLMGDTC TRGCRFCSVK TARAPPPLDV 150
    NEPVNTAKAI SSWGLDYIVL TSVDRDDLPD GGSKHIAETV REIKARNSNI 200
    FVECLVPDFR GDLECVKTIA SCGLDVYAHN IETVEKLTPF VRDRRAHYRQ 250
    TLQVLREAKN FNPNLITKSS IMLGLGETDA EVEQTMQDLR EVGVECLTLG 300
    QYMQPTKRHL KVIEYVTPEK FKHWEQRGNE LGFLYTASGP LVRSSYKAGE 350
    FFITSILANR RKSV 364
    Length:364
    Mass (Da):41,232
    Last modified:September 23, 2008 - v1
    Checksum:i9F0D9E6D2C2A9B5A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH933809 Genomic DNA. Translation: EDW18872.1.
    RefSeqiXP_002008396.1. XM_002008360.1.

    Genome annotation databases

    EnsemblMetazoaiFBtr0164196; FBpp0162688; FBgn0136227.
    GeneIDi6582704.
    KEGGidmo:Dmoj_GI13471.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH933809 Genomic DNA. Translation: EDW18872.1 .
    RefSeqi XP_002008396.1. XM_002008360.1.

    3D structure databases

    ProteinModelPortali B4KYY0.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0164196 ; FBpp0162688 ; FBgn0136227 .
    GeneIDi 6582704.
    KEGGi dmo:Dmoj_GI13471.

    Organism-specific databases

    FlyBasei FBgn0136227. Dmoj\GI13471.

    Phylogenomic databases

    InParanoidi B4KYY0.
    KOi K03644.
    OrthoDBi EOG7P2XS7.
    PhylomeDBi B4KYY0.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Evolution of genes and genomes on the Drosophila phylogeny."
      Drosophila 12 genomes consortium
      Nature 450:203-218(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Tucson 15081-1352.22.

    Entry informationi

    Entry nameiLIAS_DROMO
    AccessioniPrimary (citable) accession number: B4KYY0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: September 23, 2008
    Last modified: October 1, 2014
    This is version 36 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3