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B4KYY0

- LIAS_DROMO

UniProt

B4KYY0 - LIAS_DROMO

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Protein

Lipoyl synthase, mitochondrial

Gene
Las, GI13471
Organism
Drosophila mojavensis (Fruit fly)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi99 – 991Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi104 – 1041Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi110 – 1101Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi130 – 1301Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi134 – 1341Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi137 – 1371Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrial (EC:2.8.1.8)
Alternative name(s):
Lipoate synthase
Short name:
LS
Short name:
Lip-syn
Lipoic acid synthase
Gene namesi
Name:Las
ORF Names:GI13471
OrganismiDrosophila mojavensis (Fruit fly)
Taxonomic identifieri7230 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophila
ProteomesiUP000009192: Unassembled WGS sequence

Organism-specific databases

FlyBaseiFBgn0136227. Dmoj\GI13471.

Subcellular locationi

Mitochondrion By similarity UniRule annotation

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 364Lipoyl synthase, mitochondrialUniRule annotationPRO_0000398220
Transit peptidei1 – ?Mitochondrion Reviewed prediction

Structurei

3D structure databases

ProteinModelPortaliB4KYY0.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

InParanoidiB4KYY0.
KOiK03644.
OrthoDBiEOG7P2XS7.
PhylomeDBiB4KYY0.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B4KYY0-1 [UniParc]FASTAAdd to Basket

« Hide

MLRKLNTPTT TLVRAASTRV EKLEEIRERL SKGPNFQDFI QNSDGVKEDW    50
ENYDGKLRRE KGETQQLRLP PWLKTTIPMG KNYAKIKSQL RDLKLSTVCE 100
EARCPNIGEC WGGGEHSTQT ATIMLMGDTC TRGCRFCSVK TARAPPPLDV 150
NEPVNTAKAI SSWGLDYIVL TSVDRDDLPD GGSKHIAETV REIKARNSNI 200
FVECLVPDFR GDLECVKTIA SCGLDVYAHN IETVEKLTPF VRDRRAHYRQ 250
TLQVLREAKN FNPNLITKSS IMLGLGETDA EVEQTMQDLR EVGVECLTLG 300
QYMQPTKRHL KVIEYVTPEK FKHWEQRGNE LGFLYTASGP LVRSSYKAGE 350
FFITSILANR RKSV 364
Length:364
Mass (Da):41,232
Last modified:September 23, 2008 - v1
Checksum:i9F0D9E6D2C2A9B5A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CH933809 Genomic DNA. Translation: EDW18872.1.
RefSeqiXP_002008396.1. XM_002008360.1.

Genome annotation databases

EnsemblMetazoaiFBtr0164196; FBpp0162688; FBgn0136227.
GeneIDi6582704.
KEGGidmo:Dmoj_GI13471.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CH933809 Genomic DNA. Translation: EDW18872.1 .
RefSeqi XP_002008396.1. XM_002008360.1.

3D structure databases

ProteinModelPortali B4KYY0.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0164196 ; FBpp0162688 ; FBgn0136227 .
GeneIDi 6582704.
KEGGi dmo:Dmoj_GI13471.

Organism-specific databases

FlyBasei FBgn0136227. Dmoj\GI13471.

Phylogenomic databases

InParanoidi B4KYY0.
KOi K03644.
OrthoDBi EOG7P2XS7.
PhylomeDBi B4KYY0.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Evolution of genes and genomes on the Drosophila phylogeny."
    Drosophila 12 genomes consortium
    Nature 450:203-218(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Tucson 15081-1352.22.

Entry informationi

Entry nameiLIAS_DROMO
AccessioniPrimary (citable) accession number: B4KYY0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: September 23, 2008
Last modified: July 9, 2014
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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