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Protein

Ubiquitin carboxyl-terminal hydrolase calypso

Gene

calypso

Organism
Drosophila mojavensis (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Polycomb group (PcG) protein. Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-118' (H2AK118ub1). Does not deubiquitinate monoubiquitinated histone H2B. Required to maintain the transcriptionally repressive state of homeotic genes throughout development. The PR-DUB complex has weak or no activity toward 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei115 – 1151NucleophileBy similarity
Active sitei197 – 1971Proton donorBy similarity
Sitei212 – 2121Important for enzyme activityBy similarity

GO - Molecular functioni

  1. chromatin binding Source: UniProtKB
  2. ubiquitin-specific protease activity Source: UniProtKB

GO - Biological processi

  1. chromatin silencing Source: UniProtKB
  2. monoubiquitinated histone H2A deubiquitination Source: UniProtKB
  3. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Protein family/group databases

MEROPSiC12.A09.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase calypso (EC:3.4.19.12)
Alternative name(s):
BAP1 homolog
Gene namesi
Name:calypso
ORF Names:GI18963
OrganismiDrosophila mojavensis (Fruit fly)
Taxonomic identifieri7230 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophila
ProteomesiUP000009192: Unassembled WGS sequence

Organism-specific databases

FlyBaseiFBgn0141702. Dmoj\GI18963.

Subcellular locationi

Nucleus By similarity
Note: Localizes to PcG response elements (PREs).By similarity

GO - Cellular componenti

  1. nuclear chromatin Source: UniProtKB
  2. PR-DUB complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 461461Ubiquitin carboxyl-terminal hydrolase calypsoPRO_0000395829Add
BLAST

Interactioni

Subunit structurei

Component of the PR-DUB complex, at least composed of calypso and Asx.By similarity

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C12 family. BAP1 subfamily.Curated

Phylogenomic databases

InParanoidiB4KT51.
KOiK08588.
OMAiAFHFVSF.
OrthoDBiEOG7GN2M2.
PhylomeDBiB4KT51.

Family and domain databases

Gene3Di3.40.532.10. 1 hit.
InterProiIPR001578. Peptidase_C12_UCH.
[Graphical view]
PANTHERiPTHR10589. PTHR10589. 1 hit.
PfamiPF01088. Peptidase_C12. 1 hit.
[Graphical view]
PRINTSiPR00707. UBCTHYDRLASE.

Sequencei

Sequence statusi: Complete.

B4KT51-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNVAAGGTSA AAGSASNNNA LPMAQLADGW LELESDPGLF TLLLEDFGCH
60 70 80 90 100
DVQVEEVYDL QKPIESPYGF IFLFRWIEER RARRKIVETT AEIFVKDEEA
110 120 130 140 150
ISSIFFAQQV VPNSCATHAL LSVLLNCNEN NLQLGETLSR LKAHTKGMSP
160 170 180 190 200
ENKGLAIGNT PELACAHNSH AMPQARRRLE RTGAGVASCR FTGEAFHFVS
210 220 230 240 250
FVPINGQLFE LDGLKPYPMN HGCWEEHEDW TDKFRRVMAD RLGIATGEQD
260 270 280 290 300
IRFNLMAVVP DRRIAITHKL KMLRTNQAIV SGTLQKLLKA DEQGDDQQRP
310 320 330 340 350
DTPNTLLEPS AFTARDLQSL LKNLDTEIAI NEQHLADEND RRQMFKVDAS
360 370 380 390 400
RRTHNYDKFI CTFLSMLAHQ GVLGELVSQH LLPTKKISGQ SAANRLSKQS
410 420 430 440 450
SAATANTANS AANATAGKSQ PQQQQQQQQQ QPQSQAAKNG KSPGKTPGRR
460
RKGRNKCRKR K
Length:461
Mass (Da):51,195
Last modified:September 23, 2008 - v1
Checksum:i12F6203BE0B33A9A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH933808 Genomic DNA. Translation: EDW09571.1.
RefSeqiXP_002005636.1. XM_002005600.1.

Genome annotation databases

EnsemblMetazoaiFBtr0169688; FBpp0168180; FBgn0141702.
GeneIDi6579754.
KEGGidmo:Dmoj_GI18963.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH933808 Genomic DNA. Translation: EDW09571.1.
RefSeqiXP_002005636.1. XM_002005600.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiC12.A09.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0169688; FBpp0168180; FBgn0141702.
GeneIDi6579754.
KEGGidmo:Dmoj_GI18963.

Organism-specific databases

FlyBaseiFBgn0141702. Dmoj\GI18963.

Phylogenomic databases

InParanoidiB4KT51.
KOiK08588.
OMAiAFHFVSF.
OrthoDBiEOG7GN2M2.
PhylomeDBiB4KT51.

Family and domain databases

Gene3Di3.40.532.10. 1 hit.
InterProiIPR001578. Peptidase_C12_UCH.
[Graphical view]
PANTHERiPTHR10589. PTHR10589. 1 hit.
PfamiPF01088. Peptidase_C12. 1 hit.
[Graphical view]
PRINTSiPR00707. UBCTHYDRLASE.
ProtoNetiSearch...

Publicationsi

  1. "Evolution of genes and genomes on the Drosophila phylogeny."
    Drosophila 12 genomes consortium
    Nature 450:203-218(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Tucson 15081-1352.22.

Entry informationi

Entry nameiCALYP_DROMO
AccessioniPrimary (citable) accession number: B4KT51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: September 23, 2008
Last modified: March 4, 2015
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.