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Protein

ATP-dependent 6-phosphofructokinase

Gene

Dmoj\GI20539

Organism
Drosophila mojavensis (Fruit fly)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (Dmoj\GI20614)
  3. ATP-dependent 6-phosphofructokinase (Dmoj\GI20539), ATP-dependent 6-phosphofructokinase (Dmoj\GI20539), ATP-dependent 6-phosphofructokinase (Dmoj\GI20539), ATP-dependent 6-phosphofructokinase (Dmoj\GI20539), ATP-dependent 6-phosphofructokinase (Dmoj\GI20539)
  4. Fructose-bisphosphate aldolase (Dmoj\GI10131), Fructose-bisphosphate aldolase (Dmoj\GI10131), Fructose-bisphosphate aldolase (Dmoj\GI10131)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei27 – 271ATP; via amide nitrogenUniRule annotation
Metal bindingi121 – 1211Magnesium; catalyticUniRule annotation
Active sitei168 – 1681Proton acceptorUniRule annotation
Binding sitei203 – 2031Substrate; shared with dimeric partnerUniRule annotation
Binding sitei266 – 2661SubstrateUniRule annotation
Binding sitei294 – 2941Substrate; shared with dimeric partnerUniRule annotation
Binding sitei480 – 4801Allosteric activator fructose 2,6-bisphosphateUniRule annotation
Binding sitei575 – 5751Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation
Binding sitei638 – 6381Allosteric activator fructose 2,6-bisphosphateUniRule annotation
Binding sitei664 – 6641Allosteric activator fructose 2,6-bisphosphate; shared with dimeric partnerUniRule annotation
Binding sitei745 – 7451Allosteric activator fructose 2,6-bisphosphateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi90 – 912ATPUniRule annotation
Nucleotide bindingi120 – 1234ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotation, Transferase

Keywords - Biological processi

GlycolysisUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:Dmoj\GI20539Imported
ORF Names:Dmoj_GI20539Imported, GI20539Imported
OrganismiDrosophila mojavensis (Fruit fly)Imported
Taxonomic identifieri7230 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophila
Proteomesi
  • UP000009192 Componenti: Unassembled WGS sequence

Organism-specific databases

FlyBaseiFBgn0143275. Dmoj\GI20539.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB4KSJ9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini20 – 324305PFKInterPro annotationAdd
BLAST
Domaini411 – 696286PFKInterPro annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 392392N-terminal catalytic PFK domain 1UniRule annotationAdd
BLAST
Regioni166 – 1683Substrate bindingUniRule annotation
Regioni210 – 2123Substrate bindingUniRule annotation
Regioni300 – 3034Substrate bindingUniRule annotation
Regioni393 – 41018Interdomain linkerUniRule annotationAdd
BLAST
Regioni411 – 788378C-terminal regulatory PFK domain 2UniRule annotationAdd
BLAST
Regioni537 – 5415Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
Regioni582 – 5843Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation
Regioni670 – 6734Allosteric activator fructose 2,6-bisphosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.UniRule annotation

Phylogenomic databases

InParanoidiB4KSJ9.
KOiK00850.
OMAiVRMAIYL.
OrthoDBiEOG7ZSHV5.

Family and domain databases

HAMAPiMF_03184. Phosphofructokinase_I_E.
InterProiIPR009161. 6-Pfructokinase_euk.
IPR022953. ATP_PFK.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 2 hits.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B4KSJ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSDTNQRFL ARGSQRDKGL AVFTSGGDSQ GMNAAVRACV RMAIYLGCKV
60 70 80 90 100
YFIREGYQGM VDGGDCIQEA SWSSVSSIIH RGGTIIGSAR CKDFRERAGR
110 120 130 140 150
LKAANNLVQR GITNLVVIGG DGSLTGANLF RQEWSSLLDE LEKNKSITPE
160 170 180 190 200
QKNKYNVLHI VGLVGSIDND FCGTDMTIGT DTALHRIIEA IDAIASTAYS
210 220 230 240 250
HQRTFIMEVM GRHCGYLAIS AAIATEADFM FIPEEPAPTN WQDDLCKKLA
260 270 280 290 300
QERSAGQRLN IVIVAEGASD REGNPITAEQ VRKVIDERLK HDARITVLGH
310 320 330 340 350
VQRGGNPSAF DRVLACRMGA EATLALMEAT PESVPVVISL DGNQTVRVPL
360 370 380 390 400
MECVERTQAV AKAMAERRWA DAVKLRGRSF ERNLETYKML TRLKPPKENF
410 420 430 440 450
DEQGKGIEGY RLAVMHIGAP ACGMNAAVRS FVRNAIYRGD VVYGINDGVE
460 470 480 490 500
GLIAGNVREL GWSDVSGWVG QGGAYLGTKR TLPEGKFKEI AARLKEFKIQ
510 520 530 540 550
GLLIIGGFES YHAAGQISDQ RDNYPEFCIP IVVVPATISN NVPGTEFSLG
560 570 580 590 600
CDTGLNEITE ICDRIRQSAQ GTKRRVFVIE TMGGYSGYLA TLAGLAGGAD
610 620 630 640 650
AAYIYEEKFS IKDLQQDVYH MASKMAEGVS RGLILRNEKA SENYTTDFIY
660 670 680 690 700
RLYSEEGKGL FTCRMNILGH MQQGGSPSPF DRNMGTKMAA KCVDWLATQI
710 720 730 740 750
KNNIDSNGVV NCKSTDTATL LGIVSRQYRF TPLVDLIADT NFDQRIPKKQ
760 770 780
WWLRLRPLLR ILAKHDSAYE EEGMYITVEE ECATDAVA
Length:788
Mass (Da):86,662
Last modified:January 20, 2016 - v2
Checksum:iC510E7E23CEFB487
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH933808 Genomic DNA. Translation: EDW09504.2.
RefSeqiXP_002005569.2. XM_002005533.2.

Genome annotation databases

EnsemblMetazoaiFBtr0171264; FBpp0169756; FBgn0143275.
GeneIDi6579687.
KEGGidmo:Dmoj_GI20539.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH933808 Genomic DNA. Translation: EDW09504.2.
RefSeqiXP_002005569.2. XM_002005533.2.

3D structure databases

ProteinModelPortaliB4KSJ9.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0171264; FBpp0169756; FBgn0143275.
GeneIDi6579687.
KEGGidmo:Dmoj_GI20539.

Organism-specific databases

FlyBaseiFBgn0143275. Dmoj\GI20539.

Phylogenomic databases

InParanoidiB4KSJ9.
KOiK00850.
OMAiVRMAIYL.
OrthoDBiEOG7ZSHV5.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Family and domain databases

HAMAPiMF_03184. Phosphofructokinase_I_E.
InterProiIPR009161. 6-Pfructokinase_euk.
IPR022953. ATP_PFK.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 2 hits.
[Graphical view]
PIRSFiPIRSF000533. ATP_PFK_euk. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 2 hits.
TIGRFAMsiTIGR02478. 6PF1K_euk. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Evolution of genes and genomes on the Drosophila phylogeny."
    Drosophila 12 genomes consortium
    Nature 450:203-218(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Tucson 15081-1352.22Imported.

Entry informationi

Entry nameiB4KSJ9_DROMO
AccessioniPrimary (citable) accession number: B4KSJ9
Entry historyi
Integrated into UniProtKB/TrEMBL: September 23, 2008
Last sequence update: January 20, 2016
Last modified: April 13, 2016
This is version 57 of the entry and version 2 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Allosteric enzymeUniRule annotation, Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.