B4K9L4 (PDE6_DROMO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
cGMP-specific 3',5'-cyclic phosphodiesterase
EC=3.1.4.35

Gene names

Name:	Pde6
ORF Names:	GI10668

Organism

Drosophila mojavensis (Fruit fly) [Complete proteome]

Taxonomic identifier

7230 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila

Protein attributes

Sequence length	1116 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Has a role regulating cGMP transport in Malpighian tubule principal cells By similarity. UniProtKB Q9VFI9
Catalytic activity	Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate. UniProtKB Q9VFI9
Cofactor	Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.
Subunit structure	Interacts with PrBP By similarity. UniProtKB Q9VFI9
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side By similarity UniProtKB Q9VFI9.
Sequence similarities	Belongs to the cyclic nucleotide phosphodiesterase family. Contains 2 GAF domains.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Repeat
Ligand	Metal-binding cGMP
Molecular function	Hydrolase
PTM	Lipoprotein Methylation Prenylation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cGMP metabolic process Inferred from sequence or structural similarity. Source: UniProtKB signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3',5'-cyclic-GMP phosphodiesterase activity Inferred from sequence or structural similarity. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1113	1113	cGMP-specific 3',5'-cyclic phosphodiesterase UniProtKB Q9VFI9		PRO_0000363690
	Propeptide	1114 – 1116	3	Removed in mature form By similarity UniProtKB Q9VFI9		PRO_0000363691
Regions
	Domain	241 – 393	153	GAF 1
	Domain	425 – 611	187	GAF 2
	Compositional bias	44 – 74	31	Thr-rich
Sites
	Active site	717	1	Proton donor By similarity
	Metal binding	721	1	Divalent metal cation 1 By similarity
	Metal binding	757	1	Divalent metal cation 1 By similarity
	Metal binding	758	1	Divalent metal cation 1 By similarity
	Metal binding	758	1	Divalent metal cation 2 By similarity
	Metal binding	868	1	Divalent metal cation 1 By similarity
Amino acid modifications
	Modified residue	1113	1	Cysteine methyl ester By similarity UniProtKB Q9VFI9
	Lipidation	1113	1	S-farnesyl cysteine By similarity UniProtKB Q9VFI9

Sequences

	Sequence		Length	Mass (Da)
	B4K9L4 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 53186D5E1651993E	FASTA	1,116	124,330
	10 20 30 40 50 60 MTDVSATTGR AGDRVSSTSS EVAVETTSQA LTNGAAKEPL AAVTAEATAT ATVTATTTAT 70 80 90 100 110 120 VTATTTAATA TMATAVISKQ AKSECHSQSN NNQHVETAPS KQSSDSEASA PTTVSIPSAN 130 140 150 160 170 180 AKINSSSSGK TTATQQDVDE VARLFEEKPE AFEKWLTERA PPEALSRLHE FIESRKPHKR 190 200 210 220 230 240 PSVTSDLFQQ WMASPTVQQK SPRKLSNSSV HALPESRRHL MELDEGELFM ELIRDVANEL 250 260 270 280 290 300 DIDVLCHKIL VNVGLLTHAD RGSLFLAKGT PNNRYLVAKL FDVTQTTALK DAVTRARAEE 310 320 330 340 350 360 IIIPFGIGIA GMVAQTKQMI NIKEAYKDAR FNCEIDLKTG YTTNAILCMP ICNYEGDIIG 370 380 390 400 410 420 VAQIINKTNG CMEFDEHDVE IFRRYLTFCG IGIQNAQLFE MSVQEYRRNQ ILLNLARSIF 430 440 450 460 470 480 EEQNNLECLV TKIMTEAREL LKCERCSVFL VDLDCCEESH LEKIIEKPHQ LQQQRTPRAI 490 500 510 520 530 540 MSGDSFEEKQ NMRNRFTVLF ELGGENHAAN VSRPSINDLS HSTLAQIAQF VATTGQTVNI 550 560 570 580 590 600 CDVQDWVREH NQIRAESEID STQAILCMPI VNAQKTVIGV AQLINKASGL PFTESDASIF 610 620 630 640 650 660 EAFAIFCGLG IHNTQMYENA CKLMAKQKVA LECLSYHATA SQDQTEKLAQ DVIAEAEAYN 670 680 690 700 710 720 LYSFTFTDFD LVDDDTCRAV LRMFMQCNLV SQFHIPYDVL CRWVLSVRKN YRPVKYHNWR 730 740 750 760 770 780 HALNVAQTMF AMLKTGKMER FMTDLEILGL LVACLCHDLD HRGTNNAFQT KTESPLAILY 790 800 810 820 830 840 TTSTMEHHHF DQCVMILNSE GNNIFQALSP EDYRSVMKTV ESAILSTDLA MYFKKRNAFL 850 860 870 880 890 900 ELVENGEFDW QGEEKKDLLC GMMMTACDVS AIAKPWEVQH RVAKLVADEF FDQGDLEKLQ 910 920 930 940 950 960 LNTQPVAMMD RERKDELPKM QVGFIDVICL PLYRVLCDTF PWITPLYEGT LENRRNWQDL 970 980 990 1000 1010 1020 AEKVEMGLTW IDHDTIDKPV EEFAGCADEE IKDIEFTVTT LNCNQHGGSA GGGEDTHTPE 1030 1040 1050 1060 1070 1080 HQRSSSRLSI KKTGALGKVV RSKLSKTLYN SMDGSKPKTS LKLLESHVSE DMDDKSPTSP 1090 1100 1110 SQPHSGSVGR MSASSSTSSA GTVDKSKKRS KLCALL

References

[1]	"Evolution of genes and genomes on the Drosophila phylogeny." Drosophila 12 genomes consortium Nature 450:203-218(2007) [PubMed: 17994087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tucson 15081-1352.22.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH933806 Genomic DNA. Translation: EDW16674.1.
RefSeq	XP_002001213.1. XM_002001177.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0161393; FBpp0159885; FBgn0133432.
GeneID	6575197.
KEGG	dmo:Dmoj_GI10668.
Organism-specific databases
FlyBase	FBgn0133432. Dmoj\GI10668.
Phylogenomic databases
GeneTree	EMGT00050000002162.
InParanoid	B4K9L4.
OrthoDB	EOG41RN8T.
PhylomeDB	B4K9L4.
Family and domain databases
InterPro	IPR003018. GAF. IPR003607. Metal-dep_PHydrolase_HD_dom. IPR023088. PDEase. IPR002073. PDEase_catalytic_dom. IPR023174. PDEase_CS. [Graphical view]
Gene3D	G3DSA:1.10.1300.10. PDEase_catalytic_dom. 1 hit.
KO	K13763.
Pfam	PF01590. GAF. 2 hits. PF00233. PDEase_I. 1 hit. [Graphical view]
PRINTS	PR00387. PDIESTERASE1.
SMART	SM00065. GAF. 2 hits. SM00471. HDc. 1 hit. [Graphical view]
PROSITE	PS00126. PDEASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PDE6_DROMO

Accession

Primary (citable) accession number: B4K9L4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	September 23, 2008
Last modified:	January 25, 2012
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project