Reviewed, UniProtKB/Swiss-Prot B4K799 (SPAST_DROMO)

Names and origin

Protein names

Recommended name:
    Spastin
    EC=3.6.4.3

Gene names

Name:	spas
ORF Names:	GI24145

Organism

Drosophila mojavensis (Fruit fly)

Taxonomic identifier

7230 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila

Protein attributes

Sequence length	765 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	ATP-dependent microtubule severing protein. Stimulates microtubule minus-end depolymerization and poleward microtubule flux in the mitotic spindle. Regulates microtubule stability in the neuromuscular junction synapse By similarity.
Catalytic activity	ATP + H2O = ADP + phosphate.
Subunit structure	Homohexamer. The homohexamer is stabilized by ATP-binding. The homohexamer may adopt a ring conformation through which microtubules pass prior to being severed. Interacts with microtubules By similarity.
Subcellular location	Membrane; Single-pass membrane protein Potential. Cytoplasm › cytoskeleton › centrosome By similarity. Cytoplasm › cytoskeleton By similarity. Note: Colocalizes with cellular microtubule arrays. Localizes to chromosomes from prometaphase/metaphase to anaphase, and this requires microtubules. Localizes to discrete punctate cytoplasmic foci which may correspond to secretory vesicles By similarity.
Sequence similarities	Belongs to the AAA ATPase family. Spastin subfamily. Contains 1 MIT domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Differentiation Mitosis Neurogenesis
Cellular component	Cytoplasm Cytoskeleton Membrane Microtubule
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Developmental protein Hydrolase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW microtubule severing Inferred from sequence or structural similarity. Source: UniProtKB mitosis Inferred from electronic annotation. Source: UniProtKB-KW nervous system development Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	centrosome Inferred from sequence or structural similarity. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microtubule Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW microtubule binding Inferred from sequence or structural similarity. Source: UniProtKB microtubule-severing ATPase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 765	765	Spastin		PRO_0000367145
Regions
	Transmembrane	101 – 120	20	Potential
	Domain	217 – 293	77	MIT
	Nucleotide binding	530 – 537	8	ATP Potential
	Region	1 – 195	195	Required for localization to punctate cytoplasmic foci By similarity
	Region	192 – 765	574	Sufficient for interaction with microtubules and microtubule severing By similarity
	Region	446 – 462	17	Required for interaction with microtubules By similarity
Amino acid modifications
	Modified residue	442	1	Phosphothreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B4K799-1 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 4049BE0D4F9F20A8	FASTA	765	83,294
	10 20 30 40 50 60 MVRTKNQSSS SSASSSTKSP VKISGGGSSG ANRSRSCSEA LIDDGKTSSK LSSNRQRATI 70 80 90 100 110 120 TTTTTSTTPG SSPDDDTTDA DLTPTSGYGP RGGTSVHKQN LYVVSFPIIF LFNVLRSLIY 130 140 150 160 170 180 QLFCIFRYLY CASTKVIYRS PHRRDCNIEI VVQNSKEQQS IICPLERNTS DGIEKAQQLL 190 200 210 220 230 240 PQRQRALLPL EMATNRGGSG GYSPGPGDPL LAKQKHHHRR AFEYISKALK IDEENEGHKE 250 260 270 280 290 300 LAIELYRKGI KELEDGIAVD CWSGRGDVWD RAQRLHEKMQ TNLSMARDRL HFLALREEDL 310 320 330 340 350 360 QMQRLSLMDP PKNKQQVTSK FKQPMLVGQT NSKAAAVEPS KITMRSSGYG PKPVSGTGSS 370 380 390 400 410 420 AGTSKALQAA SGRKLTIGNK RPGNLAVANK SQTLPRNLGS KTTSTSVGAA LQRQPGKTAA 430 440 450 460 470 480 TPPAVRRQFS SGRNTPPQRS RTPINNNAAS GSGSGSGAST PMISVKGVEQ KLVQLIMDEI 490 500 510 520 530 540 VEGGAKVEWT DIAGQDVAKQ ALQEMVILPS VRPELFTGLR APAKGLLLFG PPGNGKTLLA 550 560 570 580 590 600 RAVATECSAT FLNISAASLT SKYVGDGEKL VRALFAVARH LQPSIIFIDE VDSLLSERSS 610 620 630 640 650 660 NEHEASRRLK TEFLVEFDGL PGNPEGDRIV VLAATNRPQE LDEAALRRFT KRVYVSLPEV 670 680 690 700 710 720 QTRELLLSRL LQKQGSPLDT EALARLAKIT DGYSGSDLTA LAKDAALEPI RELNVEQVKC 730 740 750 760 LDISAMRPIT EKDFHNSLKR IRRSVAPQSL NSYEKWSQDY GDITI

References

[1]	"Evolution of genes and genomes on the Drosophila phylogeny." Drosophila 12 genomes consortium Nature 450:203-218(2007) [PubMed: 17994087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tucson 15081-1352.22.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH933806 Genomic DNA. Translation: EDW14223.1.
RefSeq	XP_001998762.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6572656.
KEGG	dmo:Dmoj_GI24145.
Organism-specific databases
FlyBase	FBgn0146868. Dmoj\GI24145.
Phylogenomic databases
InParanoid	B4K799.
OrthoDB	EOG9CZBN8.
PhylomeDB	B4K799.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR003960. ATPase_AAA_CS. IPR007330. MIT. [Graphical view]
Pfam	PF00004. AAA. 1 hit. PF04212. MIT. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. SM00745. MIT. 1 hit. [Graphical view]
PROSITE	PS00674. AAA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPAST_DROMO

Accession

Primary (citable) accession number: B4K799

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 23, 2008
Last modified:	January 19, 2010
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project