Reviewed, UniProtKB/Swiss-Prot B4K6T8 (DGKH_DROMO)

Names and origin

Protein names

Recommended name:
    Diacylglycerol kinase eta
      Short name=DAG kinase eta
    EC=2.7.1.107

Gene names

ORF Names:

GI24133

Organism

Drosophila mojavensis (Fruit fly)

Taxonomic identifier

7230 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila

Protein attributes

Sequence length	1925 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA) By similarity. UniProtKB Q86XP1
Catalytic activity	ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate. UniProtKB Q86XP1
Subcellular location	Cytoplasm By similarity. UniProtKB Q86XP1
Sequence similarities	Belongs to the eukaryotic diacylglycerol kinase family. Contains 1 DAGKc domain. Contains 1 PH domain. Contains 2 phorbol-ester/DAG-type zinc fingers. Contains 1 SAM (sterile alpha motif) domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Phorbol-ester binding Repeat Zinc-finger
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	activation of protein kinase C activity by G-protein coupled receptor protein signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB intracellular signaling cascade Inferred from electronic annotation. Source: InterPro protein oligomerization Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	endosome Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW diacylglycerol binding Inferred from electronic annotation. Source: UniProtKB-KW diacylglycerol kinase activity Inferred from sequence or structural similarity. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1925	1925	Diacylglycerol kinase eta		PRO_0000375988
Regions
	Domain	82 – 175	94	PH
	Domain	350 – 486	137	DAGKc
	Domain	1862 – 1925	64	SAM
	Zinc finger	195 – 245	51	Phorbol-ester/DAG-type 1
	Zinc finger	268 – 319	52	Phorbol-ester/DAG-type 2
	Compositional bias	1131 – 1187	57	Thr-rich

Sequences

	Sequence		Length	Mass (Da)
	B4K6T8-1 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 6D8E3E275A23BCF2	FASTA	1,925	214,943
	10 20 30 40 50 60 MANLKPNTLH VDNLSPRQRS LSSGLSSACS SGSVSPVPII PIISISRDGE ESETESEIEP 70 80 90 100 110 120 EPARIFHRRM STHSKRNNNL SAIIREGYLM KHTWSFQRWR RRYFRLKRSY LYYAKDAKCD 130 140 150 160 170 180 VFDEIDLSEL CYFECSIKNV NHSFQIITPT RSLVLCADSR REMEDWLGSL KTATAPQRPR 190 200 210 220 230 240 GDSFLIDQHD ILSNHHHWYA TSHARPTYCN VCRDALSGVT SHGLSCEVCK CKVHKRCAAK 250 260 270 280 290 300 AIANCKWTTL ATVGKDIIEQ PDGSLIMPHQ WMEGNLPVSA VCAVCKKTCG SVLRLQDWRC 310 320 330 340 350 360 LWCRDTVHVA CRPQMPIVCP IGPAKLSVVP PTSVHSISTD DAWDVVSPKG NFSPLLVFVN 370 380 390 400 410 420 SKSGDNQGVK FLRRFKQLLN PAQVFDLIST GPSLGLRLFR HFEMFRILVC SGDGSVGWVL 430 440 450 460 470 480 SEIDRFNMHK QCQVAVMPLG TGNDLARVLG WGSSCDDDTH LPQILERYES ASTKMLDRWS 490 500 510 520 530 540 IMVFEKAITV PKMPKMSITT EQEALLTGMV TSANHHLRFI VETNDTQTLI SSTRSLCDTV 550 560 570 580 590 600 DELVSRICEH HKDDEQLAMK CDILRQKLTM LLDALQEEEL GTHSGDDLVA TIRSLISRSG 610 620 630 640 650 660 PLTTARPSFL NPNISIEKTE KDNINSKERR NSRSLRSSEK EALQCRANSV KRAIYNVVEH 670 680 690 700 710 720 SEPGRPKRYQ RKLSITPFEA LKIPITNSGD STPCGSPLPI IPPINIISPT METSRLTCIS 730 740 750 760 770 780 PLPDTRRDSV DENFFNSINL PAPRQFADSR RSSGVPEVIQ EMEEGASGET VYRVGRLSLS 790 800 810 820 830 840 GGANIDDAGN RLSPSSEAGE NTPTERKVDF LRVPIMTSEP IVDPLSNYRP IEVFERTYYM 850 860 870 880 890 900 AREMDKDKER TASGQVESEK EEADVNEKSE PQEPHRALVH TCNLQVPGIV VTPQSQNVYT 910 920 930 940 950 960 SENFTIIDTD AQTNTEQSSS EDLGGEASDV LSAISNEECS VASEIFDKPE SGHSLGDIIQ 970 980 990 1000 1010 1020 NLDANNFTHI DSPETSDETE PMPGESLMDD ISSVLGHDIT NALQDNTITD DTTTLCSEHA 1030 1040 1050 1060 1070 1080 GPTKPPRKKS LSALVQSKTH PRRRNSSPPR KAGLARMDSD DNPQQFGFEN IVFEIDNRCD 1090 1100 1110 1120 1130 1140 DQKIREPPRY CSLAQFVEGN DIARQSFKQL MLDRNSGDNH NDNGKNEEAD TPTNSAPTRT 1150 1160 1170 1180 1190 1200 YRNLTTTTTS DELETAIKIE INNATTNTTT STSSSISTTT TTSTTSTVKP LESAMASSTS 1210 1220 1230 1240 1250 1260 PTKKSGHGQE ISVVVRPPTP LRGDSVKPTA SSASSASLLA TSSSLLGVRT LNSSEIRRHS 1270 1280 1290 1300 1310 1320 SHAPSLAVRD YDKDKDRRHS GFNPNFLTLD PEHARFLSSS PAASRRISCG SLFKKRNQKL 1330 1340 1350 1360 1370 1380 NVKRTYGLFS VRFFVVAEPD IRLATLALIR PLIPLPNEAL PNLQTLKGSK SSLFMGSTLF 1390 1400 1410 1420 1430 1440 GFEHFSAGDK DEKPGKDKER TPTEETNRKL PIINPIVRLP NWPNLANGTG FISKCLMANA 1450 1460 1470 1480 1490 1500 DTLCAAVSPL MDPDETLLAG YHEKCVMNNY FGIGIDAKIS LDFHNKREEH PEKCRSRARN 1510 1520 1530 1540 1550 1560 YMWYGVLGSK QLLQKTCKNL EQRVQLECDG QRIPLPELQG IVILNIPSFM GGTNFWGNSS 1570 1580 1590 1600 1610 1620 KKEDIFLPPS FDDRVLEVVA VFGSVQMAAS RLINLQHHRI AQCQSVQINI LGDEEIPIQV 1630 1640 1650 1660 1670 1680 DGEAWLQPPG MIRILHKNRV QMLCRNRSLE VSLKTWQEKQ RQHSISIQRD TSSTASEHAV 1690 1700 1710 1720 1730 1740 STDEVISERE CYVLLNFIEA VSSLVKWVKF LIISHPALQH DLYEVACRAS EALESIHPQG 1750 1760 1770 1780 1790 1800 KLLEGPSLRT KLVEVIDSSR QLYDDACTLL RDRGHSLILR EDLETKLSAA LANMEMELKK 1810 1820 1830 1840 1850 1860 CSVQKCIDGK LRAYFNVLAP NEEPDGRRKS RPFWVRLRSG STAGQQQFKP PITNTREAAN 1870 1880 1890 1900 1910 1920 NWSVNEVVTW LETMQLSEYV DSFLKNDIRG KELLTLGRRD LKDLGVVKVG HVKRILQAIK DLSEN

References

[1]	"Evolution of genes and genomes on the Drosophila phylogeny." Drosophila 12 genomes consortium Nature 450:203-218(2007) [PubMed: 17994087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tucson 15081-1352.22.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CH933806 Genomic DNA. Translation: EDW14204.1.
RefSeq	XP_001998743.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6572637.
KEGG	dmo:Dmoj_GI24133.
Organism-specific databases
FlyBase	FBgn0146856. Dmoj\GI24133.
Family and domain databases
InterPro	IPR000756. Diacylglycerol_kinase_access. IPR001206. Diacylglycerol_kinase_cat. IPR011993. PH_type. IPR001849. Pleckstrin_homology. IPR002219. Prot_Kinase_C-like_PE/DAG_bd. IPR001660. SAM. IPR013761. SAM_type. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit. G3DSA:1.10.150.50. SAM_type. 1 hit.
Pfam	PF00130. C1_1. 2 hits. PF00609. DAGK_acc. 1 hit. PF00781. DAGK_cat. 1 hit. PF00169. PH. 1 hit. PF00536. SAM_1. 1 hit. [Graphical view]
SMART	SM00109. C1. 2 hits. SM00045. DAGKa. 1 hit. SM00046. DAGKc. 1 hit. SM00233. PH. 1 hit. SM00454. SAM. 1 hit. [Graphical view]
PROSITE	PS50146. DAGK. 1 hit. PS50003. PH_DOMAIN. 1 hit. PS50105. SAM_DOMAIN. 1 hit. PS00479. ZF_DAG_PE_1. 2 hits. PS50081. ZF_DAG_PE_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DGKH_DROMO

Accession

Primary (citable) accession number: B4K6T8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 26, 2009
Last sequence update:	September 23, 2008
Last modified:	October 13, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project